Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis

Xu, Haisheng; Lyu, Sunjian; Xu, Jin; Lu, Binjie; Zhao, Jing; Li, Song; Li, Yiqun; Chen, Yu-Yin

doi:10.1631/jzus.b1500098

Cited by 17 publications

(10 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…( 25 ) were due to size, reproductive period, or sampling season. In agreement with previous studies ( 31 , 32 ), two immune stimulation types led to a decrease in the THC, but no significant difference in the THC was found between genders. Similarly, Cheng et al.…”

Section: Discussionsupporting

confidence: 93%

“…It is likely the differences in the total number of hemocytes seen in the present study and that by Li et al (25) were due to size, reproductive period, or sampling season. In agreement with previous studies (31,32), two immune stimulation types led to a decrease in the THC, but no significant difference in the THC was found between genders. Similarly, Cheng et al ( 33) also reported no significant difference in the THC between genders of Macrobrachium rosenbergii, and Duchemin et al (5) observed that THC did not differ with gender in triploid or diploid C. gigas.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Gender Differences in Hemocyte Immune Parameters of Hong Kong Oyster Crassostrea hongkongensis During Immune Stress

Shi

Tuo

et al. 2021

Front. Immunol.

View full text Add to dashboard Cite

Gender differences in individual immune responses to external stimuli have been elucidated in many invertebrates. However, it is unclear if gender differences do exist in the Hong Kong oyster Crassostrea hongkongensis, one of the most valuable marine species cultivated along the coast of South China. To clarify this, we stimulated post-spawning adult C. hongkongensis with Vibrio harveyi and lipopolysaccharide (LPS). Gender-based differences in some essential functional parameters of hemocytes were studied via flow cytometry. Obvious gender-, subpopulation-, and immune-specific alterations were found in the hemocyte immune parameters of C. hongkongensis. Three hemocyte subpopulations were identified: granulocytes, semi-granulocytes, and agranulocytes. Granulocytes, the chief phagocytes and major producers of esterase, reactive oxygen species, and nitric oxide, were the main immunocompetent hemocytes. Immune parameter alterations were notable in the accumulation of granulocyte esterase activities, lysosomal masses, nitric oxide levels, and granulocyte numbers in male oysters. These results suggest that post-spawning-phase male oysters possess a more powerful immune response than females. Gender and subpopulation differences in bivalve immune parameters should be considered in the future analysis of immune parameters when studying the impact of pathogenic or environmental factors.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Gender Differences in Hemocyte Immune Parameters of Hong Kong Oyster Crassostrea hongkongensis During Immune Stress

Shi

Tuo

et al. 2021

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…ROS production, induced by phagocytosis in the process of elimination 61 , is regarded as an important mechanism for microbicidal capacities 62 . ROS is usually used to determine immunity in marine invertebrates, such as bivalves 48,63 and crustaceans 43,64 . When hemocytes were stimulated (like exposure in vitro ), respiratory burst occurred and toxic reactive oxygen species were released 65,66 .…”

Section: Discussionmentioning

confidence: 99%

Classification and characterization of hemocytes from two Asian horseshoe crab species Tachypleus tridentatus and Carcinoscorpius rotundicauda

Xie

Yan

et al. 2019

Sci Rep

View full text Add to dashboard Cite

In present study, transmission electron microscopy and flow cytometry were utilized to investigate the classification, characterization and immune functions of hemocytes from horseshoe crab, Tachypleus tridentatus and Carcinoscorpius rotundicauda . Three types of hemocytes were distinguished respectively: the granular cell, the semi-granular cell and the hyaline cell by transmission electron microscopy, while three hemocyte subpopulations (Gate 1 cell, Gate 2 cell, Gate 3 cell) were classified by flow cytometry. Hyaline cell was the major cell type with the highest nuclear-cytoplasmic ratio and granular cell and semi-granular cell showed lower ratios. Immune parameters of hemocytes in horseshoe crabs were investigated by flow cytometry. Different hemocyte subpopulations respond for diverse functions. Lysosomal contents and hemocyte mortality in Gate 3 cell subpopulation were higher than that in other subpopulations, while reactive oxygen species, phagocytosis and non-specific esterase, in Gate 1 cell subpopulation, were higher than those in other subpopulations. The hemocyte types between the two species had no significant differences in staining or morphology.

show abstract

“…Untreated cells served as negative controls. Red fluorescence was detected using a BD Accuri C6 flow cytometer (BD Biosciences, San Jose, USA) (Hahm et al, 2014;Xu et al, 2015).…”

Section: Ros Measurementmentioning

confidence: 99%

PGC-1α regulates the cell cycle through ATP and ROS in CH1 cells

Yao

et al. 2016

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Abstract:Peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α) is a transcriptional co-activator involved in mitochondrial biogenesis, respiratory capacity, and oxidative phosphorylation (OXPHOS). PGC-1α plays an important role in cellular metabolism and is associated with tumorigenesis, suggesting an involvement in cell cycle progression. However, the underlying mechanisms mediating its involvement in these processes remain unclear. To elucidate the signaling pathways involved in PGC-1α function, we established a cell line, CH1 PGC-1α, which stably overexpresses PGC-1α. Using this cell line, we found that over-expression of PGC-1α stimulated extra adenosine triphosphate (ATP) and reduced reactive oxygen species (ROS) production. These effects were accompanied by up-regulation of the cell cycle checkpoint regulators CyclinD1 and CyclinB1. We hypothesized that ATP and ROS function as cellular signals to regulate cyclins and control cell cycle progression. Indeed, we found that reduction of ATP levels down-regulated CyclinD1 but not CyclinB1, whereas elevation of ROS levels down-regulated CyclinB1 but not CyclinD1. Furthermore, both low ATP levels and elevated ROS levels inhibited cell growth, but PGC-1α was maintained at a constant level. Together, these results demonstrate that PGC-1α regulates cell cycle progression through modulation of CyclinD1 and CyclinB1 by ATP and ROS. These findings suggest that PGC-1α potentially coordinates energy metabolism together with the cell cycle.

show abstract

Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis

Cited by 17 publications

References 27 publications

Gender Differences in Hemocyte Immune Parameters of Hong Kong Oyster Crassostrea hongkongensis During Immune Stress

Gender Differences in Hemocyte Immune Parameters of Hong Kong Oyster Crassostrea hongkongensis During Immune Stress

Classification and characterization of hemocytes from two Asian horseshoe crab species Tachypleus tridentatus and Carcinoscorpius rotundicauda

PGC-1α regulates the cell cycle through ATP and ROS in CH1 cells

Contact Info

Product

Resources

About