Effect of liquid chromatography separation of complex matrices on liquid chromatography–tandem mass spectrometry signal suppression

Choi, Bernard K.; Hercules, David M.; Gusev, Arkady I.

doi:10.1016/s0021-9673(00)01052-9

Cited by 163 publications

(114 citation statements)

References 16 publications

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“…As recoveries proved to be matrix dependent, the contributions of the extraction procedure and matrix dependent ion suppression were assessed by relating the responses to standard solutions and samples spiked pre-and post-extraction [34] (Table IV). This approach was assessed using raw sewage, the most complex of aqueous samples and therefore the sample likely to contribute the greatest matrix interference.…”

Section: Methods Detection Limits Recoveries and Repeatabilitymentioning

confidence: 99%

Simultaneous determination of natural and synthetic steroid estrogens and their conjugates in aqueous matrices by liquid chromatography/mass spectrometry

Gomes¹,

Birkett²,

Scrimshaw³

et al. 2005

International Journal of Environmental Analytical Chemistry

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An analytical method for the simultaneous determination of nine free and conjugated steroid estrogens was developed with application to environmental aqueous matrices. Solid phase extraction (SPE) was employed for isolation and concentration, with detection by liquid chromatography/mass spectrometry (LC/MS) using electrospray ionisation (ESI) in the negative mode. Method recoveries for various aqueous matrices (wastewater, lake and drinking water) were determined, recoveries proving to be sample dependent. When spiked at 50 ng/l concentrations in sewage influent, recoveries ranged from 62-89 % with relative standard deviations (RSD) < 8.1 %. In comparison, drinking water spiked at the same concentrations had recoveries between 82-100 % with an RSD < 5%. Ion suppression is a known phenomenon when using ESI; hence its impact on method recovery was elucidated for raw sewage. Both ion suppression from matrix interferences and the extraction procedure has bearing on the overall method recovery. Analysis of municipal raw sewage identified several of the analytes of interest at ng/l concentrations, estriol (E3) being the most abundant. Only one conjugate, estrone 3-sulphate (E1-3S) was observed.

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Section: Methods Detection Limits Recoveries and Repeatabilitymentioning

confidence: 99%

Simultaneous determination of natural and synthetic steroid estrogens and their conjugates in aqueous matrices by liquid chromatography/mass spectrometry

Gomes¹,

Birkett²,

Scrimshaw³

et al. 2005

International Journal of Environmental Analytical Chemistry

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show abstract

“…This phenomenon, which is called the matrix effect, is attributed to the competition between matrix components and analytes for droplet formation in the solution that is sprayed (King et al, 2000;Choi et al, 2001). The effect depends on the nature of both matrices and the analytes (Cech and Enke, 2000;King et al, 2000).…”

Section: Matrix Effectmentioning

confidence: 99%

Multiresidue analysis of 47 pesticides in cooked wheat flour and polished rice by liquid chromatography with tandem mass spectrometry

Lee

Park

Kim

et al. 2008

Biomedical Chromatography

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Liquid chromatography in conjunction with tandem mass spectrometry was used to directly quantify of 47 pesticide residues from cooked wheat flour and polished rice, which are the most widely consumed cereals in the Republic of Korea. The sample clean-up was carried out according to the method established by the Korea Food and Drug Administration. The mobile phase for liquid chromatograpy separation consisted of water and 5 mM methanolic ammonium formate. Tandem mass spectroscopy experiments were performed in electrospray ionization positive mode and the multiple reaction monitoring mode. The matrix effects estimated for the 47 pesticides had a mean value of 99% and ranged from 45 to 147%. High recoveries (70-140%) and relative standard deviations (£20%) were achieved for most of the pesticides tested. The method used in this study allowed for rapid quantification and identification of low levels of pesticides in cooked wheat flour and polished rice samples. Of the screened pesticide residues, only tricyclazole and fenobucarb were found in polished rice samples. However, no samples contained residues above the MRL established by the Korea Food and Drug Administration.

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“…However for subsequent determination of method recoveries and matrix experiments 5 g of (air) dried sediment or 0.5 g of dried sludge were spiked with the addition of 5 ml acetone to aid mixing [7] and left for several hours to allow sorption processes to occur. The effects of the sediment matrix and sample preparation on analyte recovery were made by comparing the responses of the instrument to standard solutions and spiked samples ( Table 2) [32].…”

Section: Methodsmentioning

confidence: 99%

Steroid-estrogen determination in sediment and sewage sludge: a critique of sample preparation and chromatographic/mass spectrometry considerations, incorporating a case study in method development

Gomes

Avcioglu

Scrimshaw

et al. 2004

TrAC Trends in Analytical Chemistry

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Current analytical methods for determining steroids in sewage sludge and sediment are reviewed and experimental data comparing LC/MS ESI and GC/MS quantification of extracts from these matrices is presented. The effect of matrix influence on method analysis and steroid recoveries was investigated and a comparison of GC and LC determination with mass spectrometry performed. Consequently upon this, an environmental analytically robust methodology is presented and using this technique analysis of sediments determined E1 to be the main steroid (<18 ng/g), EE2 and E3 were determined at far lower concentrations with minimal detection of E2.

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Effect of liquid chromatography separation of complex matrices on liquid chromatography–tandem mass spectrometry signal suppression

Cited by 163 publications

References 16 publications

Simultaneous determination of natural and synthetic steroid estrogens and their conjugates in aqueous matrices by liquid chromatography/mass spectrometry

Simultaneous determination of natural and synthetic steroid estrogens and their conjugates in aqueous matrices by liquid chromatography/mass spectrometry

Multiresidue analysis of 47 pesticides in cooked wheat flour and polished rice by liquid chromatography with tandem mass spectrometry

Steroid-estrogen determination in sediment and sewage sludge: a critique of sample preparation and chromatographic/mass spectrometry considerations, incorporating a case study in method development

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