Effect of Lithospermi Radix on Contact Dermatitis Induced by Dinitrofluorobenzene in Mice

Kim, Hanna; Kim, Miyoung; Choi, Chan-Hun; Kim, Byung-Joo; Kim, Kyung-Yoon; Kim, Gye-Yeop; Jeong, Hyun‐Woo; Kim, Hyung Woo

doi:10.3831/kpi.2012.15.2.007

Cited by 10 publications

(5 citation statements)

References 12 publications

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“…erythrorhizon and S . indicum L had strong anti-inflammatory activity [ 20 , 21 ]. There were also reports about the active components isolated from such plants as L .…”

Section: Discussionmentioning

confidence: 99%

PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines

et al. 2017

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Psoriasis is a chronic and persistent inflammatory skin disease seriously affecting the quality of human life. In this study, we reported an ancient formula of Chinese folk medicine, the natural plant antimicrobial solution (PAMs) for its anti-inflammatory effects and proposed the primary mechanisms on inhibiting the inflammatory response in TNF-α/IFN-γ-induced HaCaT cells and imiquimod-induced psoriasis-like skin disease mouse model. Two main functional components of hydroxysafflor Yellow A and allantoin in PAMs were quantified by HPLC to be 94.2±2.2 and 262.9±12.5 μg/mL respectively. PAMs could significantly reduce the gene expression and inflammatory cytokines production of Macrophage-Derived Chemokine (MDC), IL-8 and IL-6 in TNF-α/IFN-γ-induced HaCaT cells. PAMs also significantly ameliorates the psoriatic-like symptoms in a mouse model with the evaluation scores for both the single (scales, thickness, erythema) and cumulative features were in the order of blank control < Dexamethasone < PAMs < 50% ethanol < model groups. The results were further confirmed by hematoxylin-eosin staining, RT-qPCR and immunohistochemistry. The down-regulated gene expression of IL-8, TNF-α, ICAM-1 and IL-23 in mouse tissues was consistent with the results from those of the HaCaT cells. The inhibition of psoriasis-like skin inflammation by PAMs was correlated with the inactivation of the translocation of P65 protein into cellular nucleus, indicating the inhibition of the inflammatory NF-κB signaling pathway. Taken together, these findings suggest that PAMs may be a promising drug candidate for the treatment of inflammatory skin disorders, such as psoriasis.

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“…erythrorhizon and S . indicum L had strong anti-inflammatory activity [ 20 , 21 ]. There were also reports about the active components isolated from such plants as L .…”

Section: Discussionmentioning

confidence: 99%

PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines

et al. 2017

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“…Pharmaceutical ingredients which are commonly delivered using electrospun membranes include antibiotics, anti-inflammatory drugs, proteins, nucleic acids, and herbal extracts [10,11]. Lithospermi radix (LR) has been used as a traditional therapy for burns and skin diseases such as eczema and psoriasis [12]. Recent studies showed that LR has multiple pharmacological activities, including wound-healing, antibacterial, and anti-inflammatory effects.…”

Section: Introductionmentioning

confidence: 99%

Effects of Bilayer Nanofibrous Scaffolds Containing Curcumin/Lithospermi Radix Extract on Wound Healing in Streptozotocin-Induced Diabetic Rats

Yang

Hu²,

Huang

et al. 2019

Polymers

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Impaired growth factor production, angiogenic response, macrophage function, and collagen accumulation have been shown to delay wound healing. Delayed wound healing is a debilitating complication of diabetes that leads to significant morbidity. In this study, curcumin and Lithospermi radix (LR) extract, which are used in traditional Chinese herbal medicine, were added within nanofibrous membranes to improve wound healing in a streptozotocin (STZ)-induced diabetic rat model. Gelatin-based nanofibers, which were constructed with curcumin and LR extract at a flow rate of 0.1 mL/hour and an applied voltage of 20 kV, were electrospun onto chitosan scaffolds to produce bilayer nanofibrous scaffolds (GC/L/C). The wounds treated with GC/L/C exhibited a higher recovery rate and transforming growth factor-beta (TGF-β) expression in Western blot assays. The decreased levels of pro-inflammatory markers, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), provided evidence for the anti-inflammatory effects of GC/L/C treatment. Chronic wounds treated with GC/L/C achieved better performance with a 58 ± 7% increase in recovery rate on the seventh day. Based on its anti-inflammatory and wound-healing effects, the GC/L/C bilayer nanofibrous scaffolds can be potential materials for chronic wound treatment.

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“…The three most representative chemical compounds in Atofreellage are gallic acid, caffeic acid, and hyperoside (Figure 1A–D). In fact, among the herbs comprising Atofreellage, several herbs, including R. javanica Linne, K. scoparia Schrader, C. monieri Cuss, H. cordata Thunberg, S. tenuifolia Briquet, S. flavescens Aiton, R. palmatum Linne, L. erythrorhizon Siebold et Zuccarini, T. chebula Retzins, and T. kirilowii Maximowicz partially showed potent effects on AD [32,33,34,35,36,37]. Two main compounds, gallic acid and caffeic acid, also showed inhibitory actions on histamine release by mast cells and inflammatory reactions in an AD model [28,38].…”

Section: Discussionmentioning

confidence: 99%

A Herbal Formula, Atofreellage, Ameliorates Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model

Kim

Lee

et al. 2015

Molecules

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Abstract:We evaluated the anti-atopic dermatitis (AD) effect of Atofreellage (AF), a herbal formula composed of 10 medicinal plants. AD was induced on the dorsal skin areas of NC/Nga mice (male, seven weeks old) by daily application of 2,4-dinitrochlorobenzene (DNCB) for five weeks. After three weeks of DNCB application, 200 µL of AF (0, 25, 50 or 100 mg/mL) was applied to the skin lesions. Histological findings, blood cell populations, serum levels of immunoglobulin E (IgE), histamine, pro-inflammatory cytokines, and inflammatory signaling in the skin tissue, and T-helper cell type 2 (Th 2 )-related cytokines in splenocytes were analyzed. Histopathological findings showed AF treatment notably attenuated the thickness of dorsal skin, and eosinophil infiltration. AF treatment (especially 100 mg/mL) also demonstrably ameliorated the blood cell population abnormalities, as the notable elevation of serum concentrations of IgE, histamine, TNF-α, IL-6 and IL-1β were remarkably normalized by AF treatment. Western blot analysis evidenced the apparent normalization of inflammatory signals (ERK, p38 MAP kinase, JNK, and NF-κB) in the skin tissue. Additionally, AF treatment notably attenuated the activation of Th 2 -dominant cytokines (IL-13, IL-4, and IL-5) in Con A-treated splenocytes in an ex vivo assay. In conclusion, this study provides experimental evidence for the clinical relevance of Atofreellage.

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Effect of Lithospermi Radix on Contact Dermatitis Induced by Dinitrofluorobenzene in Mice

Cited by 10 publications

References 12 publications

PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines

PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines

Effects of Bilayer Nanofibrous Scaffolds Containing Curcumin/Lithospermi Radix Extract on Wound Healing in Streptozotocin-Induced Diabetic Rats

A Herbal Formula, Atofreellage, Ameliorates Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model

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