Effect of LLLT Ga–Al–As (685 nm) on LPS-induced inflammation of the airway and lung in the rat

Abstract

The purpose of this study was to investigate the effect of low level laser therapy (LLLT) on male Wistar rat trachea hyperreactivity (RTHR), bronchoalveolar lavage (BAL) and lung neutrophils influx after Gram-negative bacterial lipopolyssacharide (LPS) intravenous injection. The RTHR, BAL and lung neutrophils influx were measured over different intervals of time (90 min, 6 h, 24 h and 48 h). The energy density (ED) that produced an anti-inflammatory effect was 2.5 J/cm(2), reducing the maximal contractile response and the sensibility of trachea rings to methacholine after LPS. The same ED produced an anti-inflammatory effect on BAL and lung neutrophils influx. The Celecoxib COX-2 inhibitor reduced RTHR and the number of cells in BAL and lung neutrophils influx of rats treated with LPS. Celecoxib and LLLT reduced the PGE(2) and TXA(2) levels in the BAL of LPS-treated rats. Our results demonstrate that LLLT produced anti-inflammatory effects on RTHR, BAL and lung neutrophils influx in association with inhibition of COX-2-derived metabolites.

The Effect of Helium-Neon Laser, Using Different Methods and Frequencies of Radiation, on Wound Healing

“…Generally, many studies indicated that, wounds irradiated by laser showed a reduction inflammatory reaction, started at the first post-operative day with a mild appearance of fibroblasts compared with control [14][15][16]. Many workers reported that laser irradiation inhibits inflammatory cells and cytokines liberation [17,18]. Kami [3] The Effect of Helium-Neon Laser, Using Different Methods and Frequencies of Radiation, on Wound Healing inflammatory effect of laser may up regulate angiogenesis around the wound causing an increase in blood flow, and thus promoter wound healing.…”

The Effect of Low Reactive-Level Laser Therapy (LLLT) with Helium-Neon Laser on Operative Wound Healing in a Rat Model

“…The irradiation period was set at 15 seconds per operative wound. The irradiation of 8.5 mW produced power density (PD) of 139 mW/cm 2 and energy density (ED) of 2.09 J/cm 2 , and irradiation of 17.0 mW produced PD of 281 mW/cm 2 and ED of 4.21 J/cm 2 were measured with a light power meter (TQ8212; Advantest, Tokyo, Japan). We assigned 5 rats to each of 11 groups: one group as a non-irradiation control, and ten irradiation groups which received either 8.5 mW or 17.0 mW of 15 seconds LLLT per operative wound a day with one of five different irradiation frequencies, i.e., daily (from the 1st to 6th day following surgery), every other day (the 1st, 3rd, and 5th day), on only the 1st day, on only the 3rd day, and on only the 5th day; the 1st day was the day following surgery.…”

“…69(8): 799-806, 2007 Low reactive level laser therapy (LLLT) [26] has been utilized clinically since the first successful cases reported by Mester [21][22][23]. Many studies have been published and various effects of LLLT have been reported such as (1) promotion of wound healing [3, 5, 14-19, 28, 29, 31-33], (2) alleviation of pain [4,7,32], (3) improvement in local circulation [20], and (4) alleviation of inflammation [2,7,8], and (5) bactericidal effects [12].In spite of this various fundamental questions have not yet been answered. Regarding wound healing, for example, it is not certain when LLLT should be performed following injury, or what level and frequency of irradiation is effective [35,36].…”

“…Many studies have been published and various effects of LLLT have been reported such as (1) promotion of wound healing [3, 5, 14-19, 28, 29, 31-33], (2) alleviation of pain [4,7,32], (3) improvement in local circulation [20], and (4) alleviation of inflammation [2,7,8], and (5) bactericidal effects [12].…”

Efeitos local e sistêmico do laser de baixa potência no limiar de dor por pressão em indivíduos saudáveis

“…O LBP vem sendo usado com o objetivo de promover efeitos analgésicos [4][5][6][7] , modulação da inflamação [8][9][10][11][12][13][14] , efeitos cicatriciais [15][16][17] e vasodilatadores locais 19 , sem os riscos de produção de calor e dano ao tecido irradiado 7,18,19 . A ação analgésica da laserterapia pode ser explicada a partir de algumas hipóteses: modulação dos processos inflamató-rios 3,9,20 , alteração da excitação e condução nervosa dos neurônios periféricos 21,22 , liberação de opioides endógenos 23,24 e aumento na síntese de serotonina 4,25 .…”