Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

Apel, Peter J.; Ma, Jun; Callahan, Michael F.; Northam, Casey; Alton, Timothy B.; Sonntag, William E.; Li, Zhongyu

doi:10.1002/mus.21485

Cited by 103 publications

(90 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We selected this strain of rat for our aging studies EFFECTS OF AGE ON SPINE MORPHOMETRY IN RATS as they weigh less than Sprague-Dawley rats, and have successfully been used as an aging animal model in nerve regeneration studies (Apel et al, 2009;Apel et al, 2010). Five animals were 85 days of age at the time of tissue harvest (classified as the ''young'' or 3-month old age group), three were 12-month old and one was 18-month old (together forming the ''aged'' group) and six were 30-month old (termed the ''geriatric'' group).…”

Section: Materials and Methods Tissue Specimensmentioning

confidence: 99%

Effects of Advanced Age on the Morphometry and Degenerative State of the Cervical Spine in a Rat Model

Laing

Cox

Tetzlaff

et al. 2011

The Anatomical Record

View full text Add to dashboard Cite

Aging causes changes in the geometry of the human cervical spine that may influence the tissue response to applied loads. Rat models are often used to study spinal cord injuries (SCI) and have the potential to enhance our understanding of the effect of age on SCI. The goal of this study was to characterize the morphometry and degenerative state of the cervical spine in Fisher 344 rats, and to determine the influence of age on these variables. Fifteen rats were split into three age groups: young adult (3 months of age), aged (12-18 months) and geriatric (30 months). Following tissue harvest we used a lCT scanner to image the cervical and upper thoracic spine from each specimen. Analysis software was used to measure variables including canal pinch diameter (the most rostral point on the dorsal aspect of a vertebral body to the most caudal aspect of the lamina on the immediately rostral vertebra), vertebral canal depth, width, and area, vertebral body height, depth, width, and area, and intervertebral disc thickness. Orthopaedic surgeons used midsagittal images to rate the degenerative state of the intervertebral discs. For all measures except disc thickness there was a significant increase (mean (SD) ¼ 15.0 (9.7)%) for the aged compared to young specimens (P < 0.05). There were significant differences between the aged and geriatric specimens for only vertebral body depth (P ¼ 0.016) and area (P ¼ 0.020). Intervertebral disc degeneration was significantly greater on the ventral aspect of the spinal column (P < 0.001), with a trend toward increased degeneration in the geriatric specimens (P ¼ 0.069). The results suggest that agerelated morphometric differences may need to be accounted for in experimental aging models of SCI in rats. Anat Rec, 294:1326Rec, 294: -1336Rec, 294: , 2011. V V C 2011 Wiley-Liss, Inc.

show abstract

Section: Materials and Methods Tissue Specimensmentioning

confidence: 99%

Effects of Advanced Age on the Morphometry and Degenerative State of the Cervical Spine in a Rat Model

Laing

Cox

Tetzlaff

et al. 2011

The Anatomical Record

View full text Add to dashboard Cite

show abstract

“…TZF canlıda en az morbidite ve immünolojik yanıt oluşturan ve aynı zamanda düşük maliyetle kök hücrenin nakledilebildiği biyomateryaldir 14,15 .TZF içerisinde bulunan İnsülin benzeri büyüme faktörü-1 (IGF-1) reseptörleri, periferik sinir aksonlarında, sinir sonlanmalarında, motor sinirlerin Schwann hücre gövdelerinde bulunur. IGF-1 sinirin uzamasını tetiklemekte ayrıca motor, duysal ve sempatik sinirlerde apopitozisi önlemektedir 16,17 . Yine TZF içinde bulunan Dönüştürücü büyüme faktörü 2-3 (TGF-2-3) Schwann hücre çoğalmasında ve farklılaşmasında kilit rol oynamaktadır 18 .…”

Section: Cerrahi Yöntemunclassified

Trombositten zengin Fibrinin periferik sinir iyileşmesi üzerindeki histopatolojik etkileri

Metineren¹,

Dülgeroğlu²,

Metineren³

2017

Dicle Tıp Dergisi

View full text Add to dashboard Cite

ÖzetAmaç: Sinir iyileşmesini hızlandırmak ve kalitesini artırmak için çeşitli materyallerle deneysel çalışmalar yapılmıştır. Çalışmamızın amacı trombositten zengin fibrinin (TZF) siyatik sinir iyileşmesi üzerindeki histopatolojik etkilerini deneysel rat modelinde araştırmaktır.Yazışma Adresi / Correspondence : Hasan Metineren, Dumlupınar Üniversitesi Tıp Fakültesi Evliya Çelebi Eğitim Araştırma Hastanesi İstiklal Cad. Okmeydanı Cd. 43040 Kütahya, Anahtar kelimeler: Trombositten zengin fibrin, sinir iyileşmesi, deneysel rat modeli, histopatoloji Sonuç: TZF gurubunun histolojik skorları, kontrol gurubuna göre istatistiksel olarak anlamlı bulundu(P=0.001). TZF sinirlerde aksonal devamlılık mevcuttu. Kontrol grubunda defektin tamamen iyileşmediği yada fibröz/granülasyon dokuyla iyileştiği gözlendi. Bol miktarda hücresel içerik ve büyüme faktörü ihtiva eden TZF sinir iyileşmesine olumlu katkı sağlamaktadır.Yöntemler: 16 Sprague Dawley rat randomize iki guruba (8 kontrol, 8 TZF) ayrıldı. Tüm ratların siyatik sinirleri kesilip 2mm defektli olarak tekrar onarıldı. TZF gurubunda defektif bölgeye TZF sarıldı. 6 haftanın sonunda ratlar sakrifiye edilerek histolojik ve immüno-histokimyasal metotlarla TZF nin kontrol gurubuna göre sinir onarımına katkısı araştırıldı.

show abstract

“…The success of axon regeneration across nerve gaps decreases with patient age, such that recovery is only relatively good for patients up to 20-25 years of age [97,[112][113][114][115]117]. For rats insulin-like growth factor 1 (IGF-1) is a potent neurotrophic factor that decreases in the distal portion of a transected nerve with increasing age [116].…”

Section: Decrease In Neurological Recovery Decreases With Increasing mentioning

confidence: 99%

“…The extent of neurological recovery decreases with increasing patient age, with best recovery being for patients up to 20-25 years of age [97,105,[112][113][114][115][116][117]. Similar decreases in the efficacy of axon regeneration are seen for rats with increasing age [118].…”

mentioning

confidence: 99%

Promoting Axon Regeneration and Neurological Recovery Following Traumatic Peripheral Nerve Injuries

Kuffler¹

2015

Int J Neurorehabilitation Eng

View full text Add to dashboard Cite

Repairing Peripheral Nerves following Traumas Nerve crushWithin several days of crushing a peripheral nerve, the injured axons begin to regenerate through their distal pathway until they reach their original targets with which they restore neurological function. The larger the number of axons that regenerate through the distal nerve, the greater the extent of neurological recovery [1][2][3]. The number of axons that regenerate, and thus neurological recovery, is influenced by the physiological state of the distal denervated nerve pathway [4][5][6][7].Following a peripheral nerve crush, the injured axons begin to regenerate within 2 days of injury [8]. They may regenerate to their targets through their original pathway in association with and promoted by its Schwann cells, the neurotrophic factors they release, and the Schwann cell extracellular matrix [1,4,[9][10][11][12][13][14]. Thus, the distal nerve provides a simple means for promoting and directing the regenerating axons to their original targets [15][16][17][18]. However, when a nerve has a gap, the gap must be bridged with a material that is permissive to and promotes axon regeneration across the entire gap to reach the distal nerve stump, through which the axons must then be able to regenerate.The Schwann cells in the denervated distal nerve release neurotrophic factors that promote axon regeneration, but also release extracellular matrix components that promote and inhibit axon regeneration [19][20][21][22][23][24][25][26]. Thus, regeneration through the distal nerve is a balance of the influences of factors that both promote and inhibit regeneration. If the Schwann cells are present, such as after a simple nerve crush, virtually 100% of the axons regenerate and they innervate all the denervated synaptic sites. If the Schwann cells within the distal nerve pathway are killed, leaving only the extracellular matrix intact, the number of axons that regenerates to their targets decreases by 94%. This is because the factors required to trigger the regenerating axons to branch at extracellular matrix branch points are missing, axons do not branch, and therefore each axon reinnervates only a single denervated muscle fiber. Thus, Schwann cells along the distal nerve pathway and a AbstractFollowing a peripheral nerve crush, or when a peripheral the nerve is transected and the nerve stumps anastomosed, neurological recovery is generally excellent. This is because the axons merely have to regenerate through the distal portion of the nerve through the existing extracellular matrix of the denervated Schwann cells that direct and promote them to their distant denervated motor and sensory targets. However, when a length of a peripheral nerve is destroyed, and anastomosis is not possible, the standard surgical repair technique is to graft a length/s of autologous sensory nerve into the gap. Neurological recovery is generally good if the nerve gap is <2 cm in length, the repair is performed <4 months post trauma, and the patients are <25 years of age. Because the nerve ...

show abstract

Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

Cited by 103 publications

References 48 publications

Effects of Advanced Age on the Morphometry and Degenerative State of the Cervical Spine in a Rat Model

Effects of Advanced Age on the Morphometry and Degenerative State of the Cervical Spine in a Rat Model

Trombositten zengin Fibrinin periferik sinir iyileşmesi üzerindeki histopatolojik etkileri

Promoting Axon Regeneration and Neurological Recovery Following Traumatic Peripheral Nerve Injuries

Contact Info

Product

Resources

About