Effect of low-dose microwave radiation on Aspergillus parasiticus

Fang, Yanpeng; Hu, Jian; Xiong, Shanbai; Zhao, Siming

doi:10.1016/j.foodcont.2011.01.004

Cited by 35 publications

(20 citation statements)

References 38 publications

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“…S1). MW radiation can increase cell membrane permeability by rupturing the spore coat and inner membrane, which results in the increased leakage of electrolytes, Ca 2+ , protein and DNA (Fang et al, 2011;Kim et al, 2009). Therefore, the total ARB concentrations of pretreated sludge reduced with the reduction of the total heterotrophic bacteria (HPC) (Fig.…”

Section: Distribution and Fate Of Arb During The Combined Mw Pretreatmentioning

confidence: 99%

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Fate of antibiotic resistance bacteria and genes during enhanced anaerobic digestion of sewage sludge by microwave pretreatment

Tong

Liu

Zheng

et al. 2016

Bioresource Technology

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Section: Distribution and Fate Of Arb During The Combined Mw Pretreatmentioning

confidence: 99%

“…Due to MW radiation can disrupt the cell membrane and cause DNA damage even better than the damaging effect of external heating (Fang et al, 2011), the total ARG concentrations reduced during the MW pretreatments with the reduction of 16S rRNA (Fig. 2a).…”

Section: Distribution and Fate Of Args During The Combined Mw Pretreamentioning

confidence: 99%

Fate of antibiotic resistance bacteria and genes during enhanced anaerobic digestion of sewage sludge by microwave pretreatment

Tong

Liu

Zheng

et al. 2016

Bioresource Technology

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“…This effect inhibits DNA repair being a plausible cause for cells inactivation. When moving from the cellular to the DNA level, microwaves can destroy DNA by denaturation, degradation, and fragmentation 10,39 .…”

Section: Resultsmentioning

confidence: 99%

“…Microwaving is commonly used in kitchen procedures such as water boiling and food heating 10,11 . It has been suggested to effectively kill bacteria, yeast, and molds on kitchen sponges 12 .…”

Section: Introductionmentioning

confidence: 99%

Anticipating xenogenic pollution at the source: Impact of sterilizations on DNA release from microbial cultures

Franco

Lin

Loosdrecht

et al. 2019

Preprint

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18The dissemination of DNA and xenogenic elements across waterways is under scientific and public 19 spotlight due to new gene-editing tools, such as do-it-yourself (DIY) CRISPR-Cas kits. Over decades, 20 prevention of spread of genetically modified organisms (GMOs), antimicrobial resistances (AMR), and 21 pathogens from transgenic systems has focused on microbial inactivation. However, sterilization 22 methods have not been assessed for DNA release and integrity. Here, we investigated the fate of 23 intracellular DNA from cultures of model prokaryotic (Escherichia coli) and eukaryotic (Saccharomyces24 cerevisiae) cells, commonly used as microbial hosts for genetic modifications, such as in white 25 biotechnology. DNA release was tracked during exposure of these cultures to conventional 26 sterilization methods. Autoclaving, disinfection with glutaraldehyde, and microwaving are used to 27 inactivate broths, healthcare equipment, and GMOs produced at kitchen table. The results show that 28 current sterilization methods are effective on microorganism inactivation but do not safeguard an 29 aqueous residue exempt of biologically reusable xenogenic material, being regular autoclaving the 30 most severe DNA-affecting method. Reappraisal of sterilization methods is required along with risk 31 assessment on the emission of DNA fragments in urban systems and nature. 32 33 34 35 3 Graphical abstract 36 37 (created with BioRender) 38 39 45 (ARGs) or pathogenicity, and their transport across urban waterways through wastewater treatment 46 plants into nature 2,3 . Novel CRISPR-Cas technologies propel the engineering of microorganisms out of 47 industry boundary with do-it-yourself (DIY) kits available at kitchen table. Uncontrolled, diffuse 48 emission of GMO materials via domestic waste streams could be a threat.49 Current sterilization methods are proven to efficiently inactivate microorganisms. However, a key 50 knowledge gap remains on their impact on DNA and its potential release into industrial, clinical, and 51 domestic sewage. Common methods used to treat industrial broths, healthcare equipment and 52 surfaces, and domestic waste primarily involve autoclaving, glutaraldehyde, and microwaving, 53 respectively. 54Several studies have shown how DNA present in food products react with different sterilization 55 procedures 4-6 . Treatments such as irradiation and autoclaving affect DNA in meat products or edible 56 seeds by decreasing the total DNA content as well as causing DNA fragmentation, degradation and 57 denaturation 7,8 . However, the impact highly depends on the cell type, the sterilization method, and 58 the process conditions. 59Temperature, pressure, pH, and sterilization times significantly exert effects on DNA quality. For 60 instance, temperatures over 100 °C have resulted in significant DNA strand clipping and irreversible 61 loss of secondary structure 4 . Normal autoclaving (121°C between 5 to 20 min) of food and crops did 62 not impeded it to be available for PCR amplification 4,5,9 63Microwaving is co...

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“…Microwaving is commonly used in kitchen procedures such as water boiling and food heating (Kim et al, 2008;Fang et al, 2011). It has been suggested to effectively kill bacteria, yeast, and molds on kitchen sponges (Sharma et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Anticipating Xenogenic Pollution at the Source: Impact of Sterilizations on DNA Release From Microbial Cultures

Calderón-Franco

Lin

Loosdrecht

et al. 2020

Front. Bioeng. Biotechnol.

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The dissemination of DNA and xenogenic elements across waterways is under scientific and public spotlight due to new gene-editing tools, such as do-it-yourself (DIY) CRISPR-Cas kits deployable at kitchen table. Over decades, prevention of spread of genetically modified organisms (GMOs), antimicrobial resistances (AMR), and pathogens from transgenic systems has focused on microbial inactivation. However, sterilization methods have not been assessed for DNA release and integrity. Here, we investigated the fate of intracellular DNA from cultures of model prokaryotic (Escherichia coli) and eukaryotic (Saccharomyces cerevisiae) cells that are traditionally used as microbial chassis for genetic modifications. DNA release was tracked during exposure of these cultures to conventional sterilization methods. Autoclaving, disinfection with glutaraldehyde, and microwaving are used to inactivate broths, healthcare equipment, and GMOs produced at kitchen table. DNA fragmentation and PCR-ability were measured on top of cell viability and morphology. Impact of these methods on DNA integrity was verified on a template of free λ DNA. Intense regular autoclaving (121 • C, 20 min) resulted in the most severe DNA degradation and lowest household gene amplification capacity: 1.28 ± 0.11, 2.08 ± 0.03, and 4.96 ± 0.28 logs differences to the non-treated controls were measured from E. coli, S. cerevisiae, and λ DNA, respectively. Microwaving exerted strong DNA fragmentation after 100 s of exposure when free λ DNA was in solution (3.23 ± 0.06 logs difference) but a minor effect was observed when DNA was released from E. coli and S. cerevisiae (0.24 ± 0.14 and 1.32 ± 0.02 logs differences with the control, respectively). Glutaraldehyde prevented DNA leakage by preserving cell structures, while DNA integrity was not altered. The results show that current sterilization methods are effective on microorganism inactivation but do not safeguard an aqueous residue exempt of biologically reusable xenogenic material, being regular autoclaving the most severe DNA-affecting method. Reappraisal of sterilization methods is required along with risk assessment on the emission of DNA fragments in urban systems and nature.

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Effect of low-dose microwave radiation on Aspergillus parasiticus

Cited by 35 publications

References 38 publications

Fate of antibiotic resistance bacteria and genes during enhanced anaerobic digestion of sewage sludge by microwave pretreatment

Fate of antibiotic resistance bacteria and genes during enhanced anaerobic digestion of sewage sludge by microwave pretreatment

Anticipating xenogenic pollution at the source: Impact of sterilizations on DNA release from microbial cultures

Anticipating Xenogenic Pollution at the Source: Impact of Sterilizations on DNA Release From Microbial Cultures

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