Effect of Low Doses and High Homeopathic Potencies in Normal and Cancerous Human Lymphocytes: An <i>In Vitro</i> Isopathic Study

Wälchli, Chantal; Baumgartner, Stephan; Bastide, M.

doi:10.1089/acm.2006.12.421

Cited by 16 publications

(16 citation statements)

References 19 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TNF-α up to 100x from 100 ng/ml elevated the level of H 2 O 2 in SK-N-SH neuroblastoma cells [30], arsenic of decimal and centesimal dilutions exerted effect in the rats [41], histamine dilutions ranging between 15-19c from 1 mg/ml inhibited human basophil degranulation [42]. In addition, normal and human leukemia T-lymphocytes responded to cadmium chloride at low doses (nM-μM; 0.2-200 ng/ml) [43]. Based on this it could be speculated, that the wound closure effect of the homeopathic remedy 0712–2 in NIH 3T3 fibroblasts may be due to the exerted properties of active ingredients at low concentrations.…”

Section: Resultsmentioning

confidence: 99%

A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates in vitro wound scratch closure of NIH 3T3 fibroblasts

Hostanska

Rostock

Melzer

et al. 2012

BMC Complement Altern Med

Self Cite

View full text Add to dashboard Cite

BackgroundDrugs of plant origin such as Arnica montana, Calendula officinalis or Hypericum perforatum have been frequently used to promote wound healing. While their effect on wound healing using preparations at pharmacological concentrations was supported by several in vitro and clinical studies, investigations of herbal homeopathic remedies on wound healing process are rare. The objective of this study was to investigate the effect of a commercial low potency homeopathic remedy Similasan® Arnica plus Spray on wound closure in a controlled, blind trial in vitro.MethodsWe investigated the effect of an ethanolic preparation composed of equal parts of Arnica montana 4x, Calendula officinalis 4x, Hypericum perforatum 4x and Symphytum officinale 6x (0712–2), its succussed hydroalcoholic solvent (0712–1) and unsuccussed solvent (0712–3) on NIH 3T3 fibroblasts. Cell viability was determined by WST-1 assay, cell growth using BrdU uptake, cell migration by chemotaxis assay and wound closure by CytoSelect ™Wound Healing Assay Kit which generated a defined “wound field”. All assays were performed in three independent controlled experiments.ResultsNone of the three substances affected cell viability and none showed a stimulating effect on cell proliferation. Preparation (0712–2) exerted a stimulating effect on fibroblast migration (31.9%) vs 14.7% with succussed solvent (0712–1) at 1:100 dilutions (p < 0.001). Unsuccussed solvent (0712–3) had no influence on cell migration (6.3%; p > 0.05). Preparation (0712–2) at a dilution of 1:100 promoted in vitro wound closure by 59.5% and differed significantly (p < 0.001) from succussed solvent (0712–1), which caused 22.1% wound closure.ConclusionResults of this study showed that the low potency homeopathic remedy (0712–2) exerted in vitro wound closure potential in NIH 3T3 fibroblasts. This effect resulted from stimulation of fibroblasts motility rather than of their mitosis.

show abstract

Section: Resultsmentioning

confidence: 99%

A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates in vitro wound scratch closure of NIH 3T3 fibroblasts

Hostanska

Rostock

Melzer

et al. 2012

BMC Complement Altern Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…Beside this, immune stimulation by natural products has been attempted in various animal models and in human cancer patients as an adjunct to chemotherapy [41]. Pre-treatment with varying potencies of cadmium has been found to significantly increase lymphocyte viability after toxic challenge compared to control cells [42]. Oliveira et al [43] have recently revealed that highly diluted tinctures can efficiently decrease tumour necrosis factor-alpha (TNF-α) release and IFN-γ production in lipopolysaccharide (LPS)-stimulated macrophages.…”

Section: Discussionmentioning

confidence: 99%

Calcarea carbonica induces apoptosis in cancer cells in p53-dependent manner via an immuno-modulatory circuit

Saha

Hossain

Mukherjee

et al. 2013

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundComplementary medicines, including homeopathy, are used by many patients with cancer, usually alongside with conventional treatment. However, the molecular mechanisms underneath the anti-cancer effect, if any, of these medicines have still remained unexplored. To this end we attempted to evaluate the efficacy of calcarea carbonica, a homeopathic medicine, as an anti-cancer agent and to delineate the detail molecular mechanism(s) underlying calcerea carbonica-induced tumor regression.MethodsTo investigate and delineate the underlying mechanisms of calcarea carbonica-induced tumor regression, Trypan blue dye-exclusion test, flow cytometric, Western blot and reverse transcriptase-PCR techniques were employed. Further, siRNA transfections and inhibitor studies were used to validate the involvement of p53 pathway in calcarea carbonica-induced apoptosis in cancer cells.ResultsInterestingly, although calcarea carbonica administration to Ehrlich’s ascites carcinoma (EAC)- and Sarcoma-180 (S-180)-bearing Swiss albino mice resulted in 30-35% tumor cell apoptosis, it failed to induce any significant cell death in ex vivo conditions. These results prompted us to examine whether calcarea carbonica employs the immuno-modulatory circuit in asserting its anti-tumor effects. Calcarea carbonica prevented tumor-induced loss of effector T cell repertoire, reversed type-2 cytokine bias and attenuated tumor-induced inhibition of T cell proliferation in tumor-bearing host. To confirm the role of immune system in calcarea carbonica-induced cancer cell death, a battery of cancer cells were co-cultured with calcarea carbonica-primed T cells. Our results indicated a "two-step" mechanism of the induction of apoptosis in tumor cells by calcarea carbonica i.e., (1) activation of the immune system of the host; and (2) induction of cancer cell apoptosis via immuno-modulatory circuit in p53-dependent manner by down-regulating Bcl-2:Bax ratio. Bax up-regulation resulted in mitochondrial transmembrane potential loss and cytochrome c release followed by activation of caspase cascade. Knocking out of p53 by RNA-interference inhibited calcarea carbonica-induced apoptosis thereby confirming the contribution of p53.ConclusionThese observations delineate the significance of immuno-modulatory circuit during calcarea carbonica-mediated tumor apoptosis. The molecular mechanism identified may serve as a platform for involving calcarea carbonica into immunotherapeutic strategies for effective tumor regression.

show abstract

“…In an in vitro study, effects of homoeopathic medicines in low doses and high potencies in normal and cancerous human lymphocytes were studied. 54 This study methodology involved pretreatment of cells with either high potencies (pool 15-20C) or low concentrations (nM-µM) of Cadmium. After 120 hours of medication, cells were exposed to Cadmium (8-80 µM).…”

Section: Dose Dependent Effects Of Homoeopathic Medicines On Cancer Cmentioning

confidence: 99%

Homoeopathy in Cancer Pain Palliation and End of Life with Future Perspectives

Sachdeva

Dey

2019

Homœopathic Links

View full text Add to dashboard Cite

Pain is a common companion of people receiving palliative care in cancer and at the end of life. Cancer pain is multifactorial and complex. Its impact can be devastating, with increased morbidity and poor quality of life, if not treated adequately. Cancer pain management is a challenging task both due to disease process and due to treatment-related side effects; therefore, it requires a holistic approach which can be covered by homoeopathic remedies. The purpose of this article is to enlighten the scope and future perspective of homoeopathy in pain palliative care and at the end of life. This article summarises the need of multidimensional approach towards the cancer pain palliation and at the end of life in which homoeopathy may play a key role of supportive care. Homoeopathy may become a valuable treatment in palliative care to provide relief and comfort to the dying patient, and therefore the fear of death may reduce and the family may be better prepared for the bereavement. Homoeopathy can well integrate with standard oncologic cares to improve patient outcome including symptom burden, quality of life and end-of-life outcomes, all achieved with low associated costs.

show abstract

Effect of Low Doses and High Homeopathic Potencies in Normal and Cancerous Human Lymphocytes: An In Vitro Isopathic Study

Abstract: High homeopathic potencies exhibit a biologic effect on cell cultures of normal primary lymphocytes. Cancerous lymphocytes (Jurkat), having lost the ability to respond to regulatory signals, seem to be fairly unresponsive to high homeopathic potencies.

Cited by 16 publications

References 19 publications

A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates in vitro wound scratch closure of NIH 3T3 fibroblasts

A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates in vitro wound scratch closure of NIH 3T3 fibroblasts

Calcarea carbonica induces apoptosis in cancer cells in p53-dependent manner via an immuno-modulatory circuit

Homoeopathy in Cancer Pain Palliation and End of Life with Future Perspectives

Contact Info

Product

Resources

About