Effect of Low-Field High-Frequency nsPEFs on the Biological Behaviors of Human A375 Melanoma Cells

Zhang, Bingyu; Kuang, Dongdong; Tang, Xuefeng; Mi, Yan; Luo, Qing; Song, Guanbin

doi:10.1109/tbme.2017.2784546

Cited by 18 publications

(7 citation statements)

References 32 publications

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“…It is important that our data are in quite good agreement with the data of Zhang [32] even he worked on human A375 melanoma cells only. He found that the nsPEFs failed to induce apoptosis of A375 melanoma cells, though it induced necrosis.…”

Section: The Influence Of One or Three Square Impulses With Field Forsupporting

confidence: 86%

Square-Wave Electric Impulses of 10 ms and 100 V/cm of Field Force, Produced by PGen-1 Impulse Generator Device, Affect the Proliferation Patterns of Different Animal Cells

Filipič¹,

Gradišnik²,

Kovacs³

et al. 2020

Electromagnetic Field Radiation in Matter

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Section: The Influence Of One or Three Square Impulses With Field Forsupporting

confidence: 86%

Square-Wave Electric Impulses of 10 ms and 100 V/cm of Field Force, Produced by PGen-1 Impulse Generator Device, Affect the Proliferation Patterns of Different Animal Cells

Filipič¹,

Gradišnik²,

Kovacs³

et al. 2020

Electromagnetic Field Radiation in Matter

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“…Our previous cell and animal experiments using high-frequency nanosecond pulses showed that this pulse form can effectively kill tumor cells and inhibit the growth of tumors in vivo . 16 These findings show that this type of pulse currently has a certain application potential for the treatment of tumors. To some extent, similar to high-frequency microsecond pulses, 3 , 4 , 17 , 18 the muscle contraction arising from high-frequency nanosecond pulses can be reduced by optimizing the pulse parameters.…”

Section: Introductionmentioning

confidence: 81%

Scaling Relationship of In Vivo Muscle Contraction Strength of Rabbits Exposed to High-Frequency Nanosecond Pulse Bursts

Tang

et al. 2018

Technol Cancer Res Treat

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We studied the influence of various parameters of high-frequency nanosecond pulse bursts on the strength of rabbit muscle contractions. Ten unipolar high-frequency pulse bursts with various field intensities E (1 kV/cm, 4 kV/cm, and 8 kV/cm), intraburst frequencies f (10 kHz, 100 kHz, and 1 MHz), and intraburst pulse numbers N (1, 10, and 100) were applied using a pair of plate electrodes to the surface skin of the rabbits’ biceps femoris, and the acceleration signal of muscle contraction near the electrode was measured using a 3-axis acceleration sensor. A time- and frequency-domain analysis of the acceleration signals showed that the peak value of the signal increases with the increasing strength of the pulse burst and that the frequency spectra of the signals measured under various pulse bursts have characteristic frequencies (at approximately 2 Hz, 32 Hz, 45 Hz, and 55 Hz). Furthermore, we processed the data through multivariate nonlinear regression analysis and variance analysis and determined that the peak value of the signal scales with the logarithm to the base 10 of ENx, where x is a value that scales with the logarithm to the base 10 of intraburst frequency (f). These results indicate that for high-frequency nanosecond pulse treatment of solid tumors in or near muscles, when the field strength is relatively high, the intraburst frequency and the intraburst pulse number require appropriate selection to limit the strength of muscle contraction as much as possible.

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“…Compared with ECT and IRE, nsPEFs can have a profound effect on the internal structure of cells due to shorter pulse duration and higher electric field intensity [ 8 , 9 ]. So far, nsPEFs have been tested in vitro and in vivo in a variety of tumors including melanoma [ 10 , 11 ], squamous cell carcinoma [ 12 ], hepatocellular carcinoma [ 13 ], pancreatic cancer [ 14 ], and breast cancer [ 15 ]. Studies have shown that nsPEFs can affect organelles [ 16 ] and plasma membrane [ 17 ], increase intracellular calcium level [ 18 ], induce cell apoptosis [ 19 ], and stimulate the body to produce stress response.…”

Section: Introductionmentioning

confidence: 99%

Effects of Nanosecond Pulsed Electric Fields in Cell Vitality, Apoptosis, and Proliferation of TPC-1 Cells

Liu

Zou

Sun

et al. 2021

Analytical Cellular Pathology

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Objective. To evaluate the effects of nanosecond pulsed electric fields (nsPEFs) with different pulse durations in cell vitality, apoptosis, and proliferation of TPC-1 cells, optimize pulse parameters and expand the application range of nsPEFs. Methods. The pulse duration of 0, 300 ns, 500 ns, and 900 ns is generated with nsPEF generator. CCK-8 was used to investigate the effect of nsPEFs on the viability of TPC-1 cells. Flow cytometry was used to evaluate the apoptosis of TPC-1 after pulse treatment. The effect of nsPEFs on the proliferation ability of TPC-1 cells was detected by 5-ethy-nyl-2 ′ -deoxyuridine. The morphological changes of TPC-1 cells after pulse treatment were observed by transmission electron microscopy. Results. NsPEFs with 900 ns pulse duration can significantly affect the viability of TPC-1 cells and inhibit the proliferation ability of TPC-1 cells. In addition, nsPEFs can also induce apoptosis of TPC-1 cells. Conclusion. NsPEFs with longer pulse duration can significantly affect the biological behavior of TPC-1 cells, such as cell viability and proliferation ability, and can also induce cell apoptosis, thereby inhibiting cell growth.

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Effect of Low-Field High-Frequency nsPEFs on the Biological Behaviors of Human A375 Melanoma Cells

Abstract: This study investigated the influence of low-field high-frequency nsPEFs on melanoma through evaluating their effects on multiple biological behaviors and is helpful in the treatment of melanoma and other tumors.

Cited by 18 publications

References 32 publications

Square-Wave Electric Impulses of 10 ms and 100 V/cm of Field Force, Produced by PGen-1 Impulse Generator Device, Affect the Proliferation Patterns of Different Animal Cells

Square-Wave Electric Impulses of 10 ms and 100 V/cm of Field Force, Produced by PGen-1 Impulse Generator Device, Affect the Proliferation Patterns of Different Animal Cells

Scaling Relationship of In Vivo Muscle Contraction Strength of Rabbits Exposed to High-Frequency Nanosecond Pulse Bursts

Effects of Nanosecond Pulsed Electric Fields in Cell Vitality, Apoptosis, and Proliferation of TPC-1 Cells

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