Effect of low-intensity focused ultrasound on endothelin-1, nitrogen monoxide and oxytocin receptor in the uterine tissues of Sprague-Dawley rats following abortion

Zhang, Yanxia; Guo, Jufang; Cai, Lin; Lü, Lei; Li, Chengzhi

doi:10.3892/br.2016.581

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…2. Ultrasound may upregulate the expression of uterine endometrium and myometrium oxytocin receptors and enhance the uterine contraction response at a molecular level [ 18 , 19 ]. 3.…”

Section: Discussionmentioning

confidence: 99%

Effect of low-intensity focused ultrasound therapy on postpartum uterine involution in puerperal women: A randomized controlled trial

Wei,

Wang,

Yue

et al. 2024

PLoS ONE

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Background Short-term poor uterine involution manifests as uterine contraction weakness. This is one of the important causes of postpartum hemorrhage, posing a serious threat to the mother’s life and safety. The study aims to investigate whether low-intensity focused ultrasound (LIFUS) can effectively shorten lochia duration, alleviate postpartum complications, and accelerate uterine involution compared with the sham treatment. Methods A multicenter, concealed, randomized, blinded, and sham-controlled clinical trial was conducted across three medical centers involving 176 subjects, utilizing a parallel group design. Enrollment occurred between October 2019 and September 2020, with a 42-day follow-up period. Participants meeting the inclusion and exclusion criteria based on normal prenatal examinations were randomly divided into the LIFUS group or the sham operation group via computer-generated randomization. Patients in the LIFUS group received usual care with the LIFUS protocol, wherein a LIFUS signal was transmitted to the uterine site through coupling gel, or sham treatment, where no low-intensity ultrasound signal output was emitted. The primary outcome, lochia duration, was assessed via weekly telephonic follow-ups post-discharge. The involution of the uterus, measured by uterine fundus height, served as the secondary outcome. Results Among the 256 subjects screened for eligibility, 176 subjects were enrolled and randomly assigned to either the LIFUS group (n = 88) or the Sham group (n = 88). Data on the height of the uterine fundus were obtained from all the patients, with 696 out of 704 measurements (99%) successfully recorded. Overall, a statistically significant difference was noted in time to lochia termination (hazard ratio: 2.65; 95% confidence interval [CI]: 1.82–3.85; P < 0.001). The decline in fundal height exhibited notable discrepancies between the two groups following the second treatment session (mean difference: −1.74; 95% CI: −1.23 to −2.25; P < 0.001) and the third treatment session (mean difference: −3.26; 95% CI: −2.74 to −3.78; P < 0.001) after delivery. None of the subjects had any adverse reactions, such as skin damage or allergies during the treatment. Conclusions This study found that LIFUS treatment can promote uterine involution and abbreviate the duration of postpartum lochia. Ultrasound emerges as a safe and effective intervention, poised to address further clinical inquiries in the domain of postpartum rehabilitation.

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“…2. Ultrasound may upregulate the expression of uterine endometrium and myometrium oxytocin receptors and enhance the uterine contraction response at a molecular level [ 18 , 19 ]. 3.…”

Section: Discussionmentioning

confidence: 99%

Effect of low-intensity focused ultrasound therapy on postpartum uterine involution in puerperal women: A randomized controlled trial

Wei,

Wang,

Yue

et al. 2024

PLoS ONE

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“…Our preliminary results showed that the participants who received LIFUS treatment had a faster decline rate and higher decline index than those who received sham treatment, indicating that LIFUS was effective in the treatment of uterine contraction. The biological effects of LIFUS mechanical stimulation, as well as the physical advantages of high penetration and focusing, make the application of LIFUS for uterine involution comfortable and painless (14,15). A slight change was observed in the rate of fundus descent.…”

Section: Discussionmentioning

confidence: 99%

Preliminary study on the effect of ultrasound on postpartum uterine involution and breast pain: a randomised controlled trial

Wei

Yue²,

Meng

et al. 2023

Preprint

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Objectives To evaluate the safety and efficacy of low-intensity focused ultrasound (LIFUS) therapy in promoting fundus descent and relieving postpartum breast pain compared with sham treatment. Methods A multicentre, randomised, sham-controlled, blinded trial was conducted. A total of 176 participants who had normal prenatal check-ups and met the inclusion and exclusion criteria were enrolled from three medical centres and subsequently randomised into the LIFUS or sham group. All participants provided three times treatment, LIFUS signal to the uterus and breast site through coupling gel, wherein a ultrasound signal output was absent in sham group. Fundal height measurement and breast pain score were performed after each treatment. The primary outcome was uterine involution, which was presented by measuring the fundal height of the uterus. The visual analogue scale (VAS) score, as a secondary outcome, was used to simultaneously assess breast pain and the correlation between breast pain and fundal height as the outcome. Results All participants were randomised into the LIFUS group (n = 88) or sham group (n = 88), of whom seven did not complete treatment. Overall, a statistically significant difference was noted in the rate and index of fundus descent after each treatment. The rate and index of fundus descent were more significant following the second (rate: 1.3 cm/d; index: 0.13, P < 0.001) and third (rate: 1.71 cm/d; index: 0.26, P < 0.001) treatments in the LIFUS group. VAS scores, which were based on the continuous variable for the baseline, first, second, and third treatments in the LIFUS (2.23, 1.16, 0.78, and 0.28 points, respectively) and sham groups (2.09, 1.64, 1.98, and 2.49 points, respectively), had a statistically significant difference between the two groups. Meanwhile, the differences in VAS score classification variables between the two groups were statistically significant. After the third treatment, a significant correlation was observed between the VAS score decrease and fundus descent rate; the more the VAS score decreased, the faster was the fundal decline rate in the LIFUS group. Conclusions LIFUS therapy is safe and effective, which contributes to accelerated uterine involution and relieved postpartum breast pain. The study has registered in the Chinese Clinical Trial Registry (ChiCTR2100049586) at 05/08/2021.

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“…and oxytocin (PGR and OXTR), involved in involution processes, were observed in metritic primiparous compared with healthy primiparous (P < 0.05). It has been hypothesized that increasing the expression of OXTR in postpartum uterine cells may help in managing incomplete uterine involution [36]. It is known that in mammals, signaling oxytocin via OXTR in the uterus results in the initiation of parturition [37].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Effect of metritis on endometrium tissue transcriptome during puerperium in Holstein lactating cows

et al. 2018

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The objective of this prospective cohort study was to evaluate the effect of parity and uterine health status postpartum on the gene expression profile of the endometrium early post-partum. Twenty-four Holstein cows were randomly selected (16 multiparous (MP) and 8 primiparous (PP)) and endometrium biopsies were collected on days 1, 3, and 6 after calving and clinically monitored for metritis. Rectal temperature was measured twice and fever was defined as a temperature ≥39.5 °C. A case of metritis was diagnosed with the presence of red-brown watery, foul-smelling uterine discharge or a purulent discharge with more than 50% pus and fever between days 1 and 6 postpartum. Cows were then retrospectively selected (cows diagnosed with metritis were paired with healthy ones) to analyze the expression of 66 genes measured on the NanoString nCounter Analysis System. The genes selected were related with adhesion, immune system, steroid and prostaglandin biosynthesis regulation, insulin metabolism and transcription factors, and nutrient transporters. The results indicated a different pattern on genes related to immune function by parity. PTX3, involved in antigen presentation, was increased in healthy MP compared with healthy PP whereas inflammatory cytokine TNFα and complement-related protein SERPING1 was upregulated in MP compared with PP (P < 0.05). As expected, presence of a metritis condition affected the expression of genes related to immune function. There was an increased expression of the antiviral factor MX2 and MYH10 gene, which is involved in macrophages recruitment, in metritic compared with healthy cows (P < 0.05). Differences in uterine involution from cows diagnosed with metritis were reflected by the downregulation of IGF1 (P < 0.10), involved in endometrium remodeling, and a possible compensatory upregulation of its receptor IGFR1 (P < 0.05). A greater expression of prostaglandins and oxytocin receptors (PGR and OXTR), involved in the involution process, were observed in metritic PP compared with healthy PP (P < 0.05). Overall, it seems that metritis significantly modulate processes closely tied with the physical involution of the uterus early post-partum (IGF1, IGFR1, PGR, OXTR), whereas both metritis and multiparous cows tended to upregulate genes related to immune response (PTX3, TNFα, SERPING1, MX2, MYH10).

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Effect of low-intensity focused ultrasound on endothelin-1, nitrogen monoxide and oxytocin receptor in the uterine tissues of Sprague-Dawley rats following abortion

Cited by 6 publications

References 13 publications

Effect of low-intensity focused ultrasound therapy on postpartum uterine involution in puerperal women: A randomized controlled trial

Effect of low-intensity focused ultrasound therapy on postpartum uterine involution in puerperal women: A randomized controlled trial

Preliminary study on the effect of ultrasound on postpartum uterine involution and breast pain: a randomised controlled trial

Effect of metritis on endometrium tissue transcriptome during puerperium in Holstein lactating cows

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