Effect of low intensity pulsed ultrasound on expression of TIMP-2 in serum and expression of mmp-13 in articular cartilage of rabbits with knee osteoarthritis

Ji, Jing-Bo; Li, Xue-Fu; Liu, Lei; Wang, Guang-Zhong; Yan, Xinfeng

doi:10.1016/j.apjtm.2015.11.003

Cited by 16 publications

(20 citation statements)

References 14 publications

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“…The results showed that the expression of caspase-3 was inhibited in chondrocytes cocultured with ADSCs group and TGF- β 1-containing culture medium group (Figures 8(a), 8(b), and 8(c)). Some studies have indicated that some matrix metalloproteinase (MMP) such as MMP3 and MMP13 can damage the chondrocytes [15, 16]. Our results showed that ADSCs reduced the destruction of matrix metalloproteinase in chondrocytes.…”

Section: Resultssupporting

confidence: 55%

Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes

Shi

Liang

Guo

et al. 2017

Stem Cells International

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Articular cartilage injury and defect caused by trauma and chronic osteoarthritis vascularity are very common, while the repair of injured cartilage remains a great challenge due to its limited healing capacity. Stem cell-based tissue engineering provides a promising treatment option for injured articular cartilage because of the cells potential for multiple differentiations. However, its application has been largely limited by stem cell type, number, source, proliferation, and differentiation. We hypothesized that (1) adipose-derived stem cells are ideal seed cells for articular cartilage repair because of their accessibility and abundance and (2) the microenvironment of articular cartilage could induce adipose-derived stem cells (ADSCs) to differentiate into chondrocytes. In order to test our hypotheses, we isolated stem cells from rabbit adipose tissues and cocultured these ADSCs with rabbit articular cartilage chondrocytes. We found that when ADSCs were cocultured with chondrocytes, the proliferation of articular cartilage chondrocytes was promoted, the apoptosis of chondrocytes was inhibited, and the osteogenic and chondrogenic differentiation of ADSCs was enhanced. The study on the mechanism of this coculture system indicated that the role of this coculture system is similar to the function of TGF-β1 in the promotion of chondrocytes.

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Section: Resultssupporting

confidence: 55%

Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes

Shi

Liang

Guo

et al. 2017

Stem Cells International

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“…Two studies showed no significant improvements in cartilage volume/thickness [18,35], but one of these further demonstrated significant improvement in pain and function after LIPUS treatment [18]. One study showed a significant increase in tissue inhibitor of metalloproteinase (TIMP) expression in LIPUS‐treated groups [30]. One study demonstrated a significant increase in CPII and a nonsignificant decrease in CTX‐II [7].…”

Section: Resultsmentioning

confidence: 99%

“…Eight studies evaluated the effect of LIPUS on expression of MMP-13, a protein involved in cartilage degradation that is overexpressed in osteoarthritis [16,[22][23][24]26,28,30,31]. These were all randomized controlled trials involving animal models.…”

Section: Resultsmentioning

confidence: 99%

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The Role of Low‐Intensity Pulsed Ultrasound on Cartilage Healing in Knee Osteoarthritis: A Review

Rothenberg

Jayaram

Naqvi

et al. 2017

PM&R

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“…Jing-Bo Ji et al [17] confirmed that the expression of serum TIMP-2 is significantly decreased in an animal model of OA. Fang-Jie Zhang et al [18] carried out an in vitro experimental study using tissue samples from 16 Chinese patients with knee OA and identified mRNA expression of TIMP-1 and TIMP-2 significantly increased cytotoxicity or apoptosis of chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

TIMP-2 SNPs rs7342880 and rs4789936 are linked to risk of knee osteoarthritis in the Chinese Han Population

Guo

Jin

et al. 2016

Oncotarget

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This study aimed to investigate whether functional polymorphisms in the tissue inhibitors of metalloproteinase-2 (TIMP-2) gene are associated with susceptibility to knee osteoarthritis (OA) in the Chinese Han population. Six TIMP-2 single nucleotide polymorphisms (SNPs) were assayed using MassARRAY in 300 patients clinically and radiographically diagnosed with knee OA and in 428 controls. Allelic and genotypic frequencies were compared between groups. Logistic regression adjusting for age and gender was used to estimate risk associations between specific genotypes and knee OA by computing odds ratios (ORs) and 95% confidence intervals (95% CIs). We found that allele “A” in rs7342880 was significantly associated with increased risk of knee OA (OR = 1.44, 95%CI = 1.09-1.91, p = 0.035). In addition, in the over-dominant model, rs4789936 correlated with reduced risk of knee OA, adjusting for age and gender (OR = 0.69, 95%CI = 0.49-0.98, p = 0.036). Finally, rs7342880 correlated with increased risk of knee OA in females. This study provides evidence that TIMP-2 is a knee OA susceptibility gene in the Chinese population and a potential diagnostic and preventive marker for the disease.

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Effect of low intensity pulsed ultrasound on expression of TIMP-2 in serum and expression of mmp-13 in articular cartilage of rabbits with knee osteoarthritis

Abstract: The inner patellar ligament defect method is a mature method to establish the rabbit OA model, with high success rate. The expression of serum TIMP-2 in the OA model group is significantly decreased. LIPUS can up-regulate TIMP-2 and down-regulate MMP-13.

Cited by 16 publications

References 14 publications

Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes

Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes

The Role of Low‐Intensity Pulsed Ultrasound on Cartilage Healing in Knee Osteoarthritis: A Review

TIMP-2 SNPs rs7342880 and rs4789936 are linked to risk of knee osteoarthritis in the Chinese Han Population

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