Objective: In office methods for immediate relief of dentinal hypersensitivity (DH) has long been an area of research. This study compared the efficacy of 660 nm diode laser, 980 nm diode laser, and amorphous calcium phosphate–casein phosphopeptide (ACP–CPP) agent in the treatment of DH.Materials and Methods: A total of 39 patients with minimum three hypersensitive teeth in at least one quadrant were selected and randomly divided into three groups; Group A, B, and C patients were treated by 660 nm diode laser, 980 nm diode laser, and ACP–CPP agent, respectively. All the hypersensitive teeth were stimulated with tactile; thermal and air stimuli, and the pretreatment and posttreatment hypersensitivity scores were evaluated with the numeric rating scale (NRS) at baseline, 30 min, 1 week, 1 month, 3 months, and 6 months. The Shapiro–Wilk test was used to determine the uniformity of the data. The Chi‐square (χ2) test of significance was used to compare proportions. For intergroup comparison, the Kruskal–Wallis test and the Mann–Whitney U test were utilized.Results: At 30 min and 1 week, the 980 nm laser was more effective than the 660 nm laser, but there was no statistically significant difference between the two groups at 1, 3, and 6 months. Throughout the trial period, the 660 nm and 980 nm diode lasers were more effective than the ACP–CPP agent in lowering DH.Conclusions: 660 nm diode laser, 980 nm diode laser, and ACP–CPP agent showed positive prospective as effective desensitizers when utilized as a clinical procedure.