Effect of Low-Level Laser Therapy on Inflammatory Reactions during Wound Healing: Comparison with Meloxicam

Viegas, Vinícius Nery; Abreu, Marcelo Emir Requia; Viezzer, Christian; Machado, Denise Cantarelli; Filho, Manoel Sant’Anna; Silva, Daniel; Pagnoncelli, Rogério Miranda

doi:10.1089/pho.2007.1098

Cited by 47 publications

(43 citation statements)

References 14 publications

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“…In addition, this technique is non-invasive, nonpharmacological, and has few side effects (MEDEIROS et al, 2010). There is still no consensus on the antiinflammatory activity of PBMT because previous studies have indicated a possible anti-inflammatory activity at the initial stages of the wound-healing process (MEDRADO et al, 2008;VILELA et al, 2012;, whereas other authors did not detect this activity (VIEGAS et al, 2007). There is also the report of the modulation of inflammatory mediators (FIORIO et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The antiinflammatory action of PBMT in the early stages of tissue repair is associated with the decrease in the number of inflammatory cells (MEDRADO et al, 2008;VILELA et al, 2012). However, some studies did not find any difference in the number of polymorphonuclear cells between the group treated with PBMT and the control group and consequently did not corroborate this anti-inflammatory activity (VIEGAS et al, 2007). Therefore, there is no consensus on the antiinflammatory potential of PBMT in skin wound healing.…”

Section: Introductionmentioning

confidence: 99%

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Effect of gallium arsenide low-level laser therapy on the inflammatory phase of skin wound healing in rats

Carvalho

Bizo

Toma

et al. 2017

AVB

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Article historyThe use of photobiomodulation therapy (PBMT) has shown promising results for the inflammatory modulation of tissue repair. This study aimed to evaluate the antiinflammatory potential of gallium arsenide (GaAs) PBMT in skin wound healing via (1) measurement of the temperature on the surface of the skin wound; (2) white blood cell count; and (3) histopathological examination. Skin lesions were induced on the dorsum of 20 Wistar rats, after which the animals were divided into two groups: a treatment group, subjected to GaAs PBMT, and a control group. Thermography was performed daily in all of the study animals until the end of the experiment. On the fourth day after lesion induction, whole blood was collected (white blood cell count), animals were euthanized, and skin lesions were biopsied (histopathological examination). There were no differences in the number of leukocytes and in the histopathological evaluations between the groups; however, the thermography analysis indicated an increase in temperature in the treated group. The antiinflammatory activity of GaAs PBMT was not confirmed. The increase in temperature on the surface of the skin lesions after LLLT requires further elucidation because this result could not be justified by the direct action of PBMT.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of gallium arsenide low-level laser therapy on the inflammatory phase of skin wound healing in rats

Carvalho

Bizo

Toma

et al. 2017

AVB

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“…11,20,21,[24][25][26][27][28][29] A growing body of evidence suggests that LPT has anti-inflammatory action and accelerates tissue repair. 18,19,21,22 However, these effects are dependent on wavelength, output power, 28,35 and energy density.…”

Section: Discussionmentioning

confidence: 99%

“…22 The effects of LPT on the healing process of skin wounds have been studied, particularly using the dorsum skin model to investigate the effect of different irradiation parameters. 11,20,21,[23][24][25][26][27] A growing body of evidence suggests that LPT has anti-inflammatory action and accelerates tissue repair. 18,19,21,22 However, these effects are dependent on irradiation parameters such as wavelength, output power, 28,29 and energy density.…”

Section: Introductionmentioning

confidence: 99%

Influence of different energy densities of laser phototherapy on oral wound healing

et al. 2013

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Abstract. The aim of the present prospective study was to evaluate the impact of laser phototherapy (LPT) on the healing of oral ulcers. Different power densities were used on oral wounds in Wistar rats (n ¼ 72) randomly divided into three groups: control (0 J∕cm 2 ), 4 J∕cm 2 laser, and 20 J∕cm 2 laser. Ulcers (3 mm in diameter) were made on the dorsum of the tongue with a punch. Irradiation with an indium-gallium-aluminum-phosphide laser (660 nm; output power: 40 mW; spot size: 0.04 cm 2 ) was performed once a day in close contact with the ulcer for 14 consecutive days. A statistically significant acceleration in healing time was found with wounds treated with 4 J∕cm 2 LPT. Moreover, striking differences were found in the ulcer area, healing percentage, degree of reepithelialization, and collagen deposition. The most significant changes occurred after 5 days of irradiation. Based on the conditions employed in the present study, LPT is capable of accelerating the oral mucosa wound-healing process. Moreover, faster and more organized reepithelialization and tissue healing of the oral mucosa were achieved with an energy density of 4 J∕cm 2 in comparison to 20 J∕cm 2 .

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“…Novas possibilidades terapêuticas estão sendo preconizadas utilizando a terapia laser de baixa intensidade (LLLT) no processo de reparo tecidual. Esta terapia demonstra ser um recurso eficaz na diminuição do tempo de reposição tecidual, ou seja, melhora a qualidade da reparação tecidual e acelera o processo de cicatrização [6][7][8][9] . Modelos experimentais de diabetes em murinos estão sendo amplamente estudados a fim de esclarecer o mecanismo fisiopatológico da doença.…”

Section: Introductionunclassified

Inibição da expressão de ciclooxigenase 2 em feridas cutâneas de camundongos NOD submetidos à terapia a laser de baixa intensidade

Rocha

Júnior²,

Aarestrup

et al. 2012

J. vasc. bras.

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ResumoContexto: A terapia a laser de baixa intensidade (LLLT) tem sido relatada como importante moduladora da cicatrização de feridas cutâneas aumentando a proliferação fibroblástica associada ao aumento da expressão da citocina fator transformador de crescimento-β2 (TGF-βB2). Objetivo: No presente estudo foram avaliados os efeitos da LLLT sobre a expressão da enzima ciclooxigenase 2 (COX2) no sítio do reparo tecidual utilizando o modelo experimental com camundongos diabéticos não obesos (NOD) para estudar a cicatrização de feridas cutâneas. Métodos: Foram utilizados 30 camundongos NOD, destes 14 ficaram diabéticos e foram divididos em dois grupos: o grupo I (n=7) foi submetido a um procedimento cirúrgico de feridas cutâneas e o grupo II (n=7) foi submetido a um procedimento cirúrgico de feridas cutâneas e tratados com LLLT. O grupo II foi submetido à LLLT nos seguintes parâmetros: 15 mW de potência, dose de 3,8 J/cm 2 e tempo de aplicação de 20 segundos. Após sete dias do ato cirúrgico e após aplicação do laser, os animais foram eutanasiados com sobredose de anestesia e amostras das feridas foram colhidas para posterior análise histopatológica, histomorfométrica e imuno-histoquímica. Resultados: A LLLT promoveu a inibição da expressão da COX2 em feridas cutâneas de camundongos diabéticos. Conclusão: Em conjunto, os resultados sugeriram que a LLLT é capaz de modular negativamente a expressão da enzima COX2 contribuindo para o controle da resposta inflamatória em feridas cutâneas de camundongos NOD.Palavras-chave: camundongos endogâmicos NOD; ciclooxigenase 2; terapia a laser de baixa intensidade. AbstractBackground: Low-level laser therapy (LLLT) has been reported to modulate the healing of wounds by inducing an increase in fibroblast number associated with increased expression of the cytokine transforming growth factor-β 2 (TGF-β 2 ). Objective: In the present study, the effect of LLLT on expression of COX2 at the site of tissue repair was evaluated, using an experimental model with non obese diabetic mice (NOD) to study cutaneous wound healing. Methods: Thirty NOD mice were used, of which 14 were diabetic and were divided into two groups: group I (n=7) underwent a surgical procedure of skin wounds and group II (n=7) underwent a surgical procedure of skin wounds and treated with LLLT. Group II was submitted to LLLT in the following parameters: 15 mW of power, dose of 3.8 J/cm 2 and exposure time of 20 seconds. Seven days after surgery and after laser application, animals were euthanized with an overdose of anesthesia and tissue samples were collected for subsequent histological analysis, histomorphometry and immunohistochemistry. Results: The LLLT has promoted the inhibition of COX2 expression in skin wounds in mice diabetic. Taken together the results suggest that LLLT modulate the expression of COX2 improved the control of inflammatory reaction in cutaneous wound lesions in NOD mice. Conclusion: Taken together, the results suggested that LLLT is able to negatively modulate the expression of COX2 enzyme contri...

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Effect of Low-Level Laser Therapy on Inflammatory Reactions during Wound Healing: Comparison with Meloxicam

Abstract: LLLT improves the quality of histologic repair and is useful during wound healing. However, with the methods used in this study the laser energy did not minimize tissue inflammatory reactions.

Cited by 47 publications

References 14 publications

Effect of gallium arsenide low-level laser therapy on the inflammatory phase of skin wound healing in rats

Effect of gallium arsenide low-level laser therapy on the inflammatory phase of skin wound healing in rats

Influence of different energy densities of laser phototherapy on oral wound healing

Inibição da expressão de ciclooxigenase 2 em feridas cutâneas de camundongos NOD submetidos à terapia a laser de baixa intensidade

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