Effect of Low-Level Laser therapy on the fungal proliferation of<i>Candida albicans</i>

Carneiro, Vanda Sanderana Macêdo; Araújo, Natália; Menezes, Rebeca Ferraz de; Moreno, Lara Marques Magalhães; Santos-Neto, Alexandrino dos Pereira; Gerbi, Marleny Elizabeth Márquez de Martínez

doi:10.1117/12.2214269

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…The results of this study illustrate descriptively that LLLT can reduce the number of C. albicans colonies, although the results were not statistically significant for LLLT at a wavelength of 976 nm and doses of 5 and 10 J/ cm 2 (Table 6). These results are in line with research by Carneiro et al 30 who examined the effect of LLLT on the number of C. albicans colonies with C. albicans samples derived from AIDS patients in vitro. They observed a decrease in C. albicans colonies for a red laser (685 nm) at doses of 6, 8, and 10 J/cm 2 and an infrared laser (830 nm) at doses of 6, 8, 10, and 12 J/cm 2 .…”

Section: Discussionsupporting

confidence: 92%

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In Vitro Effect of Low-level Laser Therapy on Candida albicans Colonies Isolated From Patients Undergoing Radiotherapy for Head and Neck Cancer

Hasanah,

Sufiawati,

Kusumadjati

et al. 2024

J Lasers Med Sci

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Introduction: Studies on head and neck cancer (HNC) patients undergoing radiotherapy have revealed increased numbers of Candida spp., leading to oral candidiasis and oral mucositis. The effects of laser therapy on Candida spp. have been studied with varied results. This study aimed to investigate the effect of low-level laser therapy (LLLT) on C. albicans colonies isolated from HNC patients undergoing radiotherapy. Methods: This study included a treated group, wherein 11 C. albicans isolates were obtained from the saliva of HNC patients undergoing radiotherapy at a dose exceeding 30 Gy. The control groups consisted of an untreated negative control and a positive control treated with nystatin. The treatment groups were subjected to LLLT in continuous mode for 50 seconds at a wavelength of 976 nm, 0.1 W at a dose of 5 J/cm2 for Laser 1 (L1) group and 0.2 W at a dose of 10 J/cm2 for Laser 2 (L2) group. The tests were conducted using C. albicans inoculum equivalent to a 0.5-McFarland suspension on 96-well plates. All test group inoculums were then cultured on Sabouraud dextrose agar (SDA), and the colony count was assessed at 104 CFU/mL. Results: LLLT at 5 and 10 J/ cm2 reduced C. albicans colonies by 7.01% and 10.94%, respectively, but the reductions were not statistically significant (P>0.05). Nystatin eliminated all the colonies. Conclusion: LLLT shows potential for reducing C. albicans colonies in HNC patients undergoing radiotherapy. However, further studies with varied parameters and methods are necessary.

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Section: Discussionsupporting

confidence: 92%

“…Statistical results showed no significant difference (P > 0.05). 30 Najafi et al also examined the in vitro effect of a 940 nm Ga-Al-Ar diode laser on the number of C. albicans (ATCC 18804) colonies. They used doses of 38 and 76 J/cm 2 for both 10 4 and 10 6 concentrations, with exposure times of 30 and 60 seconds.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effect of Low-level Laser Therapy on Candida albicans Colonies Isolated From Patients Undergoing Radiotherapy for Head and Neck Cancer

Hasanah,

Sufiawati,

Kusumadjati

et al. 2024

J Lasers Med Sci

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“…Some experiments with Candida albicans have also been published in other countries, and basically the results have been slight to moderate stimulation of growth .…”

Section: Pbm In Non‐mammalian Hosts and Model Organismsmentioning

confidence: 98%

Non‐mammalian Hosts and Photobiomodulation: Do All Life‐forms Respond to Light?

Hamblin

Huang

Heiskanen

2018

Photochem & Photobiology

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Photobiomodulation (PBM), also known as low-level laser (light) therapy, was discovered over 50 years ago, but only recently has it been making progress toward wide acceptance. PBM originally used red and near-infrared (NIR) lasers, but now other wavelengths and non-coherent light-emitting diodes (LEDs) are being explored. The almost complete lack of side effects makes the conduction of controlled clinical trials relatively easy. Laboratory research has mainly concentrated on mammalian cells (normal or cancer) in culture, and small rodents (mice and rats) as models of different diseases. A sizeable body of work was carried out in the 1970s and 1980s in Russia looking at various bacterial and fungal cells. The present review covers some of these studies and a recent number of papers that have applied PBM to so-called "model organisms." These models include flies (Drosophila), worms (Caenorhabditis elegans), fish (zebrafish) and caterpillars (Galleria). Much knowledge about the genomics and proteomics, and many reagents for these organisms already exist. They are inexpensive to work with and have lower regulatory barriers compared to vertebrate animals. Other researchers have studied different models (snails, sea urchins, Paramecium, toads, frogs and chickens). Plants may respond to NIR light differently from visible light (photosynthesis and photomorphogenesis) but PBM in plants has not been much studied. Veterinarians routinely use PBM to treat non-mammalian patients. The conclusion is that red or NIR light does indeed have significant biologic effects conserved over many different kingdoms, and perhaps it is true that "all life-forms respond to light."

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The effect of low level laser on number of Candida albicans colonies in-vitro: a new finding

et al. 2019

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Background Candida albicans is a commensal organism that causes a wide variety of diseases in humans. One of these diseases is oral candidiasis, which occurs at a high recurrence rate in spite of available treatments. The purpose of this study was to compare the effect of low-level laser therapy (LLLT) with the use of nystatin on in-vitro growth of Candida albicans . Method We prepared two samples of Candida albicans at different concentrations: 10 4 viable cells/ml and 10 6 viable cells/ml. Specimens from each sample were divided into a control group, a nystatin-treated group, and a group treated with LLLT. The control group was cultured without any intervention. The second group was treated with nystatin and the solution was vibrated for 30 s or 60 s. The third group was irradiated with a gallium-aluminum-argon (Ga-Al-Ar) diode laser (Epic 10; Biolase Inc.)in continuous mode using a wavelength of 940 nm and a power of 1 W for 30 s or 60 s (38 J/cm 2 and 76 J/cm 2 ). The specimens from the nystatin group and the LLLT group were cultured and the number of colony-forming units (CFU/ml) for each group was counted and compared. Results Nystatin completely eliminated the colonies (0 colonies) in all specimens. There was an increase in the number of colonies in the LLLT group for both cell concentrations at 30 s and at 60 s. However, this increase was statistically significant only for a concentration of 10 4 viable cells/ml at an exposure time of 30s. The increase in the concentration of 10 6 viable cells/ml at both 30 s and 60 s was statistically significant compared with the control group, although the highest number of colonies remained after an exposure time of 60s. Conclusion LLLT led to an increase in the growth of Candida colonies. However, there was no significant difference related to the exposure time between the different cell concentrations.

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Effect of Low-Level Laser therapy on the fungal proliferation ofCandida albicans

Cited by 4 publications

References 15 publications

In Vitro Effect of Low-level Laser Therapy on Candida albicans Colonies Isolated From Patients Undergoing Radiotherapy for Head and Neck Cancer

In Vitro Effect of Low-level Laser Therapy on Candida albicans Colonies Isolated From Patients Undergoing Radiotherapy for Head and Neck Cancer

Non‐mammalian Hosts and Photobiomodulation: Do All Life‐forms Respond to Light?

The effect of low level laser on number of Candida albicans colonies in-vitro: a new finding

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