Effect of low-level α-radiation on bioluminescent assay systems of various complexity

Rozhko, T.V.; Kudryasheva, Nadezhda S.; Кузнецов, А. М.; Vydryakova, G. A.; Bondareva, L. G.; Bolsunovsky, A. Ya.

doi:10.1039/b614162p

Cited by 33 publications

(55 citation statements)

References 28 publications

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“…In the late 1980s, the test was standardized in Germany as a method for pollutant detection. Later, it was modified by different researchers and adapted for their specific purposes [2][3][4][5][6]. Now, the bacterial bioluminescent assay is a traditional and important biotechnological application of the bioluminescence phenomenon [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…In this work, the effect of gamma-ray irradiation on a recombinant Escherichia coli strain was studied. Later, the bacterial bioluminescence was used to monitor toxicity of alpha-emitting [4,5,14] and betaemitting [15] radionuclides.…”

Section: Introductionmentioning

confidence: 99%

“…Bioluminescent kinetics was already studied in solutions of Am(NO 3 ) 3 [4,5,14], tritium-labeled aminoacide valinе [23], and in tritiated water, HTO [15]. Reactive Oxygen Species (ROS), produced as secondary products of radioactive decay in aquatic solutions, were suggested to be responsible for biological effects of 241 Am [24].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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Effects of Americium-241 (241Am), alpha-emitting radionuclide of high specific radioactivity, and tritium (3H), beta-emitting radionuclide, on luminous bacteria Photobacterium phosphoreum were compared. Bioluminescence intensity served as a marker of bacterial physiological activity. Three successive stages in the bioluminescence response to 241Am and 3H were found under conditions of lowdose irradiation: (1) absence of effects, (2) activation, and (3) inhibition. They were interpreted in terms of bacterial response to stressfactor as stress recognition, adaptive response/syndrome, and suppression of physiological function (i.e. radiation toxicity). Times of bioluminescence activation (TBA) and inhibition (TBI) were suggested as parameters to characterize hormesis and toxic stages in a course of chronic low-dose irradiation of the microorganisms. Values of TBA and TBI of 241Am were shorter than those of 3H, revealing higher impact of alpha-irradiation (as compared to beta-irradiation) under comparable radiation doses. Increases of peroxide concentration and NADH oxidation rates in 241Am aquatic solutions were demonstrated; these were not found in tritiated water. The results reveal a biological role of reactive oxygen species generated in water solutions as secondary products of the radioactive decay. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha- and beta-emitting radionuclides in aquatic solutions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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“…Earlier, the low-dose conditions were provided in experiments with luminous marine bacteria in the solutions of alpha- (Rozhko at al., 2007; and beta- (Selivanova et al, 2013) emitting radionuclides; three bioluminescence kinetics stages (threshold effect, activation and inhibition) were observed there. Probably, a lower life-time of the bacteria under the conditions of the current experiment prevented from observation of the third (inhibition) stage.…”

Section: The Effect Of Tritium On Bioluminescence Of Marine Bacteriamentioning

confidence: 99%

“…In the course of recent years, extensive research has been performed into the influence of ionizing radiation on bioluminescence of marine bacteria (Min et al, 2003;Rozhko et al, 2007Rozhko et al, , 2008Rozhko et al, , 2011Alexandrova et al, 2011;Selivanova et al, 2013Selivanova et al, , 2014. The studies of the effects of alpha-and beta-emitting radionuclides on luminous bacteria under the conditions of chronic low-dose irradiation are summarized in (Kudryasheva and Rozko, 2015).…”

Section: Introductionmentioning

confidence: 99%

On the mechanism of biological activation by tritium

Rozhko

Badun

Razzhivina

et al. 2016

Journal of Environmental Radioactivity

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The mechanism of biological activation by beta-emitting radionuclide tritium was studied. Luminous marine bacteria were used as a bioassay to monitor the biological effect of tritium with luminescence intensity as the physiological parameter tested. Two different types of tritium sources were used: HTO molecules distributed regularly in the surrounding aqueous medium, and a solid source with tritium atoms fixed on its surface (tritium-labeled films, 0.11, 0.28, 0.91, and 2.36 MBq/cm2). When using the tritium-labeled films, tritium penetration into the cells was prevented. The both types of tritium sources revealed similar changes in the bacterial luminescent kinetics: a delay period followed by bioluminescence activation. No monotonic dependences of bioluminescence activation efficiency on specific radioactivities of the films were found. A 15-day exposure to tritiated water (100 MBq/L) did not reveal mutations in bacterial DNA. The results obtained give preference to a "non-genomic" mechanism of bioluminescence activation by tritium. An activation of the intracellular bioluminescence process develops without penetration of tritium atoms into the cells and can be caused by intensification of trans-membrane cellular processes stimulated by ionization and radiolysis of aqueous media. ON THE MECHANISM OF BIOLOGICAL ACTIVATION BY TRITIUM AbstractThe mechanism of biological activation by beta-emitting radionuclide tritium was studied. Luminous marine bacteria were used as a bioassay to monitor the biological effect of tritium with luminescence intensity as the physiological parameter tested. Two different types of tritium sources were used: HTO molecules distributed regularly in the surrounding aqueous medium, and a solid source with tritium atoms fixed on its surface 0.11, 0.28, 0.91, and 2.36 MBq/cm 2 ). When using the tritiumlabeled films, tritium penetration into the cells was prevented. The both types of tritium sources revealed similar changes in the bacterial luminescent kinetics: a delay period followed by bioluminescence activation. No monotonic dependences of bioluminescence activation efficiency on specific radioactivities of the films were found. A 15-day exposure to tritiated water (100 MBq/L) did not reveal mutations in bacterial DNA. The results obtained give preference to a "non-genomic" mechanism of bioluminescence activation by tritium. An activation of the intracellular bioluminescence process develops without penetration of tritium atoms into the cells and can be caused by intensification of trans-membrane cellular processes stimulated by ionization and radiolysis of aqueous media.

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Stimulation of luminescence of mycelium of luminous fungusNeonothopanus nambiby ionizing radiation

et al. 2014

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The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi. We report the effect of X-irradiation on the luminescence of fungus Neonothopanus nambi. Experiments were performed with mycelium on a home-built setup based on an X-ray tube and monochromator/photomultiplier tube. Application of X-rays does not change the emission spectrum, but after approximately 20 min of continuous irradiation, light production from unsupported mycelium starts growing and increases up to approximately five times. After peaking, its level decreases irrespective of the presence of X-irradiation. After staying at a certain level, light production collapses to zero, which is not related to the drying of the mycelium or thermal impact of radiation. The observed shape of kinetics is characteristic of a multistage and/or chain reaction. The time profile of light production must reflect the current levels of radicals present in the system and/or the activity of enzyme complexes involved in light production.

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Effect of low-level α-radiation on bioluminescent assay systems of various complexity

Cited by 33 publications

References 28 publications

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

On the mechanism of biological activation by tritium

Stimulation of luminescence of mycelium of luminous fungusNeonothopanus nambiby ionizing radiation

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