Effect of low methionine formula on levels of IL-1β Serum and IL-1β gene expression in knee joint cartilage tissues of normal rabbits and ACl induction OA models

Sutjiati, Endang; Kusworini,; Wirjatmadi, Bambang; Kalim, Handono

doi:10.5958/0976-5506.2018.01403.1

Cited by 1 publication

(2 citation statements)

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“…This can be seen from the results of clinical observations of changes in body weight and rectal temperature of rats tested for 72 hours. There was no significant change in body weight and temperature between the 2 groups and the controls 11 .…”

Section: Discussionmentioning

confidence: 71%

“…In another study, it was using 5% SDS, it also obtained a good microscopic deselularization result but because SDS is cytotoxic, it is necessary to conduct additional leaching which takes longer time with Phospat Buffered Salinese (PBS) for 4 days. However, this washing extension has an adverse effect on Extra Cellular Matrix (ECM) cartilage, which causes ECM to appear broken between the second and third days 11 .…”

Section: Discussionmentioning

confidence: 99%

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Biocompatibility Test of Decellularized Cartilage Bovine Scaffold in Vitro and In Vivo

2020

IJFMT

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Background: Incidence of cartilage defects was reported at 65.00% of the routine arthroscopy procedures. Dr.Soetomo Teaching Hospital Network Bank developed a cartilage scaffold from bovine that has been deselularized decellularized cartilage bovine scaffold (DCBS). It takes a study of biocompatibility test to prove that DCBS is not cytotoxic to cells and not generate a host response. Objective:To analyze the biocompatibility of decellularized cartilage bovine scaffold (DCBS) in vivo and in vitro. Methods and Materials:The subjects were experimental rats and experimental with in vitro and in vivo trials. The subjects were treated by toxicity test with MTT assay, irritation test using Draize Scale, acute/ pyogenic systemic toxicity test by observing changes in body weight and temperature, and implantation test by observing fibrous capsule formation and Immunoglobulin G. Data was analyzed by using Kolmogorovsmirnov, Independent T-test, Mutivariate Analysis of Variance (MANOVA), and Mann Whitney. Result:The percentage of viable DCBS (81.78%) and CBS (92.45%) was ≥70% of the control; thus, it is non-toxic to the cell. Draize scale in each group was in grade 0. Changes in body weight (p = 0.981) and temperature (p >0.05) had no significant association between groups. There was a significant difference mean of the fibrous capsule (p = 0.000) thickness and no significant difference in the mean number of Immunoglobulin G which was formed at week 1 (p = 0.87) and week 4 (p = 0.63) after implantation between CCBS and DCBS. Conclusion:The biocompatibility test shows that DCBS is compatible as a biomaterial.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%