Effect of low voltage electrical stimulation on protein and quality changes in bovine muscles during postmortem aging

Effects of high voltage electrical stimulation on the rate of pH decline, meat quality and color stability in chilled beef carcasses

“…Similarly, many studies report that electrical stimulation accelerates glycolysis and causes a rapid pH decrease [9][10][11] . Morton [12] .…”

Efficacy of Carcass Electrical Stimulation in Meat Quality Enhancement: A Review

“…Trial conducted by Solomon et al (1986) revealed that young bovine carcasses when subjected to ES at 1.5 A, 550 V and 20 pulses for 2 s produced more tender meat than non-stimulated carcasses as evaluated by sensory panels. In contrast, Kim et al (2013) reported that low voltage ES at 90 min after exsanguination had no effect on tenderness development and proteolysis in bovine gracilis M., Longissimus dorsi M. and semimembranosus M. Similarly, studies conducted by Botha et al (2009) inferred that low voltage ES offered no improvement in the quality attributes of ostrich. Using twenty-two ovine carcasses from castrated Doper weighing 40 to 50 kg, Davel et al (2003) investigated the influence of electrical stimulation at 20 V, 45 Hz for 45 s on sensory features of mutton.…”

“…There are discrepancies among various reports on the influence of ES on meat quality. While some authors reported positive effects (Mckenna et al, 2003; Cetin and Topcu, 2009; Nazli et al, 2010), others reported no effect (Wiklund et al, 2001; Botha et al, 2009; Kim et al, 2013) or in some cases deleterious effects (Hector et al, 1992; den Hertog-Meischke et al, 1997; Simmons et al, 2008) of ES on meat quality. These ostensible paradoxes have not been fully resolved in spite of partial insights given by recent reports.…”

Top-Down Proteomics and Farm Animal and Aquatic Sciences

“…In fact, in these fields of research, two-dimensional gel electrophoresis (2DE) has been termed “the workhorse” for proteomics research [2]. Numerous examples may be found in the literature on AASP research using 2DE, including single stain gels [15,16,17,18] and 2DE-DIGE (difference gel electrophoresis) [19]. Additional alternative gel techniques such as blue native polyacrylamide (PAGE) gels, particularly suitable for membrane proteomics, and the study of protein complexes and protein–protein interactions [20], such as those in the mitochondria [21], have also been used.…”