Objective: to find a modern technology to produce honey fortified naturally with lycopene extracted from tomatoes and to examine the bioactivity of the novel product. Materials and methods: Lycopene was extracted from tomatoes, tomatoes peels and tomatoes juice, the presence of lycopene and its concentrations was detected using UV-vis., the extracted lycopene was added to natural honey in different concentrations, the presence of lycopene in naturally fortified honey produced using HPLC technology was detected and active ingredients were detected using GC-MASS technology. Results: the tomatoes peels gave the highest concentration of lycopene compared to other parts, it gave a value of 0.52 relay factor (Rf) in addition to the appearance of a peak at a retention time (Rt) of 11.3 minutes corresponding to the standard lycopene top retention time, when performing a spectrophotometry between (300-600nm), pure lycopene gave three peaks at 440,460, 503nm wavelengths, and the retention coefficient (COD) is 0.98, 1.00, and 0.96 respectively. The effectiveness of enzymes (SOD) and (GPX) when treating with CCl4, it was found that the effectiveness of the two enzymes increased when using honey and lycopene in its various forms, as shown in results, a number of raw lycopene components appear in fortified honey such as; Ibuprofen, anti-inflammatory, sedative and substance, 1-Tetrazol-2-ylethanone, antimicrobial, anti-inflammatory, analgesic, anti-epileptic, anti-viral, antihypertensive, anti-malarial drug, anti- anxiety, depression, antihistamine, and 4H-Pyran-4 -one, 2,3-dihydro-3,5-dihydroxy-6-methyl which is an anti- cancer substance. Conclusion: the new honey fortified with lycopene shows a high activity against microbes, and also can be applied as anti-depression, anti-allergic and more.
