Effect of lyophilization on liposomal encapsulation of salmon calcitonin

Swaminathan, Janani; Ehrhardt, Carsten

doi:10.3109/08982104.2014.899366

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…This effect was not observed for large liposomes due to the small quantity of non-entrapped drug in the original samples. Similar results have been found for salmon calcitonin liposomes [ 40 ]. The increases in Dh and PDI values observed in rehydrated liposomes from samples containing L, S, or T do not affect their suitability for pulmonary drug delivery.…”

Section: Discussionsupporting

confidence: 90%

Lyoprotective Effects of Mannitol and Lactose Compared to Sucrose and Trehalose: Sildenafil Citrate Liposomes as a Case Study

et al. 2021

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The lyoprotective effects of mannitol and lactose have been evaluated in the production of sildenafil citrate liposomes. Liposomes were prepared by mixing the components under ultrasonic agitation, followed by a transmembrane pH gradient for remote drug loading. Mannitol and lactose, as compared to sucrose and trehalose, were used as the stabilizing agents, and different freeze-drying cycles were assayed. The remaining moisture and the thermal characteristics of the lyophilized samples were analyzed. Size, entrapment efficiency, biocompatibility, and cell internalization of original and rehydrated liposomes were compared. The type of additive did not affect the biocompatibility or cell internalization, but did influence other liposome attributes, including the thermal characteristics and the remaining moisture of the lyophilized samples. A cut-off of 5% (w/w) remaining moisture was an indicator of primary drying completion—information useful for scaling up and transfer from laboratory to large-scale production. Lactose increased the glass transition temperature to over 70 °C, producing lyoprotective effects similar to those obtained with sucrose. Based on these results, formulations containing liposomes lyophilized with lactose meet the FDA’s requirements and can be used as a biocompatible and biodegradable vehicle for the pulmonary delivery of therapeutic doses of sildenafil citrate.

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Section: Discussionsupporting

confidence: 90%

Lyoprotective Effects of Mannitol and Lactose Compared to Sucrose and Trehalose: Sildenafil Citrate Liposomes as a Case Study

et al. 2021

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“…It was shown that dehydration–rehydration of the vesicles have numerous advantages such as comparatively better encapsulation efficiency and lower probable destabilization to the encapsulated drug. Moreover, freeze-drying is usually utilized as a means to increase the physical stability of BBVs. , Use of DEAE-Dx as a steric stabilizer for CBBVs caused an increase in particle size and increase in ZP due to the effect of cationic polymer DEAE-Dx . The slightly higher EE% of DEAE-Dx-stabilized BBVs may be due to the larger vesicle particle size compared to CBBVs .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, freeze-drying is usually utilized as a means to increase the physical stability of BBVs. 45,46 Use of DEAE-Dx as a steric stabilizer for CBBVs caused an increase in particle size and increase in ZP due to the effect of cationic polymer DEAE-Dx. 22 The slightly higher EE% of DEAE-Dxstabilized BBVs may be due to the larger vesicle particle size compared to CBBVs.…”

Section: ■ Discussionmentioning

confidence: 99%

Superiority of DEAE-Dx-Stabilized Cationic Bile-Based Vesicles over Conventional Vesicles for Enhanced Hepatic Delivery of Daclatasvir

et al. 2019

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The purpose of our study was to improve the delivery of a direct-acting antiviral drug, daclatasvir, to the site of action, liver tissues, using physically and biologically stable cationic bile-based vesicles. Accordingly, cationic bile-based vesicles were prepared as pro-bile-based vesicles and diethylaminoethyl dextran (DEAE-Dx)-stabilized bile-based vesicles to increase their stability without negatively affecting their hepatic affinity. The prepared bile-based vesicles were characterized for particle size, polydispersity index, ζ-potential, in vitro daclatasvir release, and ex vivo permeation using non-everted gut sac intestine. The in vivo biodistribution was experimented after oral administration utilizing the radiolabeling assay, where the liver showed the highest accumulation of the DEAE-Dx-stabilized bile-based vesicles after 4 h, reaching a value of 4.6% ID/g of the total oral administered dose of the labeled drug compared to drug solution, pro-bile-based vesicles, and cationic bile-based vesicles where the accumulation was 0.19, 1.3, and 0.31% ID/g, respectively. DEAE-Dx-stabilized bile-based vesicles increased the drug deposition into the liver about 42-fold compared to oral solution. The high physical stability and the high resistance to opsonization and clearance show that DEAE-Dx-stabilized bile-based vesicles could be efficiently applied for enhancing daclatasvir delivery to the liver after oral administration.

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A comprehensive production method of self-cryoprotected nano-liposome powders

Gala

Khan

Elhissi

et al. 2015

International Journal of Pharmaceutics

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Effect of lyophilization on liposomal encapsulation of salmon calcitonin

Abstract: Lyophilization resulted in an increase in encapsulation efficiency of sCT in PEGylated liposomes, even in the absence of a cryoprotectant, due to presence of micellar vesicles.

Cited by 6 publications

References 28 publications

Lyoprotective Effects of Mannitol and Lactose Compared to Sucrose and Trehalose: Sildenafil Citrate Liposomes as a Case Study

Lyoprotective Effects of Mannitol and Lactose Compared to Sucrose and Trehalose: Sildenafil Citrate Liposomes as a Case Study

Superiority of DEAE-Dx-Stabilized Cationic Bile-Based Vesicles over Conventional Vesicles for Enhanced Hepatic Delivery of Daclatasvir

A comprehensive production method of self-cryoprotected nano-liposome powders

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