Effect of lyophilization on the bacterial cellulose produced by different Komagataeibacter strains to adsorb epicatechin

Chen, Siqian; Cao, Xiao; Li, Zhaofeng; Zhu, Jie; Li, Lin

doi:10.1016/j.carbpol.2020.116632

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…Generally, the yield of BC is affected by bacterial strain, medium composition and culture conditions (e.g., pH, agitation rate, oxygen availability) [12][13][14][15]. Additionally, high-yielding bacterial strains also contribute to a more homogenous BC fibril network structure and corresponding mechanical properties (i.e., extensibility, stiffness, viscoelasticity and poroelasticity) [14,16,17]. Previous reports have shown Komagataeibacter hansenii ATCC 53582 contains two additional operons involved in manipulating the Acs operons in cellulose synthesis [8,18], potentially producing a higher yield of BC [17,19,20].…”

Section: Introductionmentioning

confidence: 99%

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Effect of pH Buffer and Carbon Metabolism on the Yield and Mechanical Properties of Bacterial Cellulose Produced by Komagataeibacter hansenii ATCC 53582

Chen

Cao

et al. 2021

J. Microbiol. Biotechnol.

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Bacterial cellulose (BC) is widely used in the food industry for products such as nata de coco. The mechanical properties of BC hydrogels, including stiffness and viscoelasticity, are determined by the hydrated fibril network. Generally, Komagataeibacter bacteria produce gluconic acids in a glucose medium, which may affect the pH, structure and mechanical properties of BC. In this work, the effect of pH buffer on the yields of Komagataeibacter hansenii strain ATCC 53582 was studied. The bacterium in a phosphate and phthalate buffer with low ionic strength produced a good BC yield (5.16 and 4.63 g/l respectively), but there was a substantial reduction in pH due to the accumulation of gluconic acid. However, the addition of gluconic acid enhanced the polymer density and mechanical properties of BC hydrogels. The effect was similar to that of the bacteria using glycerol in another carbon metabolism circuit, which provided good pH stability and a higher conversion rate of carbon. This study may broaden the understanding of how carbon sources affect BC biosynthesis.

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Section: Introductionmentioning

confidence: 99%

“…, extensibility, stiffness, viscoelasticity and poroelasticity) [ 14 , 16 , 17 ]. Previous reports have shown Komagataeibacter hansenii ATCC 53582 contains two additional operons involved in manipulating the Acs operons in cellulose synthesis [ 8 , 18 ], potentially producing a higher yield of BC [ 17 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of pH Buffer and Carbon Metabolism on the Yield and Mechanical Properties of Bacterial Cellulose Produced by Komagataeibacter hansenii ATCC 53582

Chen

Cao

et al. 2021

J. Microbiol. Biotechnol.

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“…On the liquid surface, the bacteria form BC that protects them from dry-out, irradiation, lack of oxygen, and pathogens [ 6 ]. Many examples of scientific articles describing the production of BC using various bacterial species and subspecies, media composition (carbon and nitrogen source, pH), and reaction conditions leading to materials of various shapes and properties can be found in literature [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…After harvesting and washing the BC hydrogel, the excessive water can be removed by several drying methods: air drying, oven drying, draining with water-absorbing material, supercritical drying, or lyophilization. Generally, lyophilization and supercritical drying are milder drying methods; the material maintains its nanostructure, and therefore shows a higher water swelling ratio and porosity [ 6 , 8 , 9 , 46 , 47 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Drying Method and Argon Plasma Modification of Bacterial Nanocellulose on Keratinocyte Adhesion and Growth

et al. 2021

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Due to its nanostructure, bacterial nanocellulose (BC) has several advantages over plant cellulose, but it exhibits weak cell adhesion. To overcome this drawback, we studied the drying method of BC and subsequent argon plasma modification (PM). BC hydrogels were prepared using the Komagataeibacter sucrofermentans (ATCC 700178) bacteria strain. The hydrogels were transformed into solid samples via air-drying (BC-AD) or lyophilization (BC-L). The sample surfaces were then modified by argon plasma. SEM revealed that compared to BC-AD, the BC-L samples maintained their nanostructure and had higher porosity. After PM, the contact angle decreased while the porosity increased. XPS showed that the O/C ratio was higher after PM. The cell culture experiments revealed that the initial adhesion of human keratinocytes (HaCaT) was supported better on BC-L, while the subsequent growth of these cells and final cell population density were higher on BC-AD. The PM improved the final colonization of both BC-L and BC-AD with HaCaT, leading to formation of continuous cell layers. Our work indicates that the surface modification of BC renders this material highly promising for skin tissue engineering and wound healing.

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Effect of Ex Situ Modification of Bacterial Cellulose with Organosilane Coupling Agent on Drug Delivery Properties

Nascimento,

Silva,

de Lima

et al. 2024

J Polym Environ

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Effect of lyophilization on the bacterial cellulose produced by different Komagataeibacter strains to adsorb epicatechin

Cited by 7 publications

References 35 publications

Effect of pH Buffer and Carbon Metabolism on the Yield and Mechanical Properties of Bacterial Cellulose Produced by Komagataeibacter hansenii ATCC 53582

Effect of pH Buffer and Carbon Metabolism on the Yield and Mechanical Properties of Bacterial Cellulose Produced by Komagataeibacter hansenii ATCC 53582

Influence of Drying Method and Argon Plasma Modification of Bacterial Nanocellulose on Keratinocyte Adhesion and Growth

Effect of Ex Situ Modification of Bacterial Cellulose with Organosilane Coupling Agent on Drug Delivery Properties

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