Effect of lysozyme or modified lysozyme fragments on DNA and RNA synthesis and membrane permeability of Escherichia coli

Pellegrini, Antonio; Thomas, Ursula; Wild, P.; Schraner, Elisabeth M.; Fellenberg, R. von

doi:10.1016/s0944-5013(00)80040-3

Cited by 70 publications

(54 citation statements)

References 30 publications

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“…Lysozyme has also been shown to form pores in gram-negative bacteria through cationic and hydrophobic properties. This activity is independent of the muramidase activity (25,36).…”

Section: Discussionmentioning

confidence: 86%

Association of Salmonella enterica SerovarEnteritidis YafD with Resistance to Chicken EggAlbumen

Killoran

Riley

2003

Infect Immun

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Salmonella enterica serovar Enteritidis is a major cause of food-borne diseases in industrialized countries. The incidence of S. enterica serovar Enteritidis infections has increased substantially in recent decades, and S. enterica serovar Enteritidis is now one of the leading serovars of Salmonella in the United States. A unique epidemiological characteristic of S. enterica serovar Enteritidis is its association with chicken shell eggs, since approximately 80% of all human gastrointestinal diseases can be traced to contaminated egg products. Eggs are contaminated when bacteria from reproductive tissues of infected hens are packaged into the eggs and persist inside the hostile egg albumen environment. Therefore, resistance to egg albumen is an important aspect in the transmission of S. enterica serovar Enteritidis. We identified a gene, yafD from S. enterica serovar Enteritidis, whose overexpression conferred upon S. enterica serovar Typhimurium enhanced resistance to egg albumen, while disruption of this gene in S. enterica serovar Enteritidis rendered the organism more susceptible to egg albumen. YafD is homologous to members of an exonuclease-endonuclease-phosphatase family, including some enzymes involved in DNA repair. Furthermore, we discovered that egg albumen has nuclease activities and uses both circular and linear DNA as substrates. We propose that YafD provides a survival advantage to S. enterica serovar Enteritidis in eggs by repairing DNA damage caused by egg albumen and that it may be one of the biologic determinants that contribute to the epidemiological association of S. enterica serovar Enteritidis with egg products.

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“…Lysozyme has also been shown to form pores in gram-negative bacteria through cationic and hydrophobic properties. This activity is independent of the muramidase activity (25,36).…”

Section: Discussionmentioning

confidence: 86%

Association of Salmonella enterica SerovarEnteritidis YafD with Resistance to Chicken EggAlbumen

Killoran

Riley

2003

Infect Immun

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“…The principal antimicrobial component of the egg albumen is believed to be ovotransferrin that chelates iron that is necessary for bacteria to proliferate (1, 7, 58). More recently, both ovotransferrin and lysozyme were reported to form pores in gram-negative bacteria through cationic peptides (28,29,46). Moreover, we have discovered that egg albumen may also damage bacterial DNA.…”

Section: Discussionmentioning

confidence: 98%

“…Such cationic peptides are present in egg albumen. Both ovotansferrin and lysozyme have been demonstrated to have cationic activity and can form pores in the membranes of gram-negative bacteria (28,29,46). Any change in the charge or structure of LPS in Salmonella enterica serovar Enteritidis could alter its interaction with ovotransferrin, lysozyme, or other cationic peptides and cause Salmonella enterica serovar Enteritidis to become more susceptible to egg albumen.…”

Section: Discussionmentioning

confidence: 99%

Identification of Genes Associated with Survival ofSalmonella entericaSerovar Enteritidis in Chicken Egg Albumen

Clavijo

Loui

Andersen

et al. 2006

Appl Environ Microbiol

115

119

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Salmonella enterica consists of over 2,000 serovars that are major causes of morbidity and mortality associated with contaminated food. Despite similarities among serovars of Salmonella enterica, many demonstrate unique host specificities, epidemiological characteristics, and clinical manifestations. One of the unique epidemiological characteristics of the serovar Enteritidis is that it is the only bacterium routinely transmitted to humans through intact chicken eggs. Therefore, Salmonella enterica serovar Enteritidis must be able to persist inside chicken eggs to be transmitted to humans, and its survival in egg is important for its transmission to the human population. The ability of Salmonella enterica serovar Enteritidis to survive in and transmit through eggs may have contributed to its drastically increased prevalence in the 1980s and 1990s. In the present study, using transposon-mediated mutagenesis, we have identified genes important for the association of Salmonella enterica serovar Enteritidis with chicken eggs. Our results indicate that genes involved in cell wall structural and functional integrity, and nucleic acid and amino acid metabolism are important for Salmonella enterica serovar Enteritidis to persist in egg albumen. Two regions unique to Salmonella enterica serovar Enteritidis were also identified, one of which enhanced the survival of a Salmonella enterica serovar Typhimurium isolate in egg albumen. The implication of our results to the serovar specificity of Salmonella enterica is also explored in the present study.

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“…When the lysozyme is expressed in transgenic bacteria or supplied to cultured cells, the protein has been suggested to act via a membrane-disrupting activity (Düring et al, 1999). Although the muramidase activity of lysozyme damage to the peptidoglycan layer is well documented, the protein, variants or even fragments, may also interact non-enzymically with the bacterial membrane (Düring et al, 1999;Pellegrini et al, 2000). This additional bactericidal activity could be an important feature of the wEa1h lysozyme (H. Salm & K. Geider, unpublished).…”

Section: Discussionmentioning

confidence: 99%

Expression of bacteriophage ϕEa1h lysozyme in Escherichia coli and its activity in growth inhibition of Erwinia amylovora

2004

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A 3·3 kb fragment from Erwinia amylovora phage ϕEa1h in plasmid pJH94 was previously characterized and found to contain an exopolysaccharide depolymerase (dpo) gene and two additional ORFs encoding 178 and 119 amino acids. ORF178 (lyz) and ORF119 (hol) were found to overlap by 19 bp and they resembled genes encoding lysozymes and holins. In nucleotide sequence alignments, lyz had structurally conserved regions with residues important for lysozyme function. The lyz gene was cloned into an expression vector and expressed in Escherichia coli. Active lysozyme was detected only when E. coli cells with the lyz gene and a kanamycin-resistance cassette were grown in the presence of kanamycin. Growth of Erw. amylovora was inhibited after addition of enzyme exceeding a threshold for lysozyme to target cells. When immature pears were soaked in lysates of induced cells, symptoms such as ooze formation and necrosis were retarded or inhibited after inoculation with Erw. amylovora.

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Effect of lysozyme or modified lysozyme fragments on DNA and RNA synthesis and membrane permeability of Escherichia coli

Cited by 70 publications

References 30 publications

Association of Salmonella enterica SerovarEnteritidis YafD with Resistance to Chicken EggAlbumen

Association of Salmonella enterica SerovarEnteritidis YafD with Resistance to Chicken EggAlbumen

Identification of Genes Associated with Survival ofSalmonella entericaSerovar Enteritidis in Chicken Egg Albumen

Expression of bacteriophage ϕEa1h lysozyme in Escherichia coli and its activity in growth inhibition of Erwinia amylovora

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