Effect of magnevist conjugated dendrimer on Bax/Bcl2 gene expression levels in MCF-7 cancer cells

Abstract

KEYWORDS Magnevist conjugated dendrimerReal-time PCR MCF-7 ApoptosisCorona protein ABSTRACTIn recent years, investigating pharmacogenetics and effects of corona protein nanomaterial in biological applications has been seriously considered for the treatment of human diseases. In this study, the mechanism of apoptosis induced by Magnevist conjugated dendrimer was investigated to compare Magnevist as a contrasting agent on MCF-7 cells. Cytotoxicity effects of new Magnevist conjugated dendrimer was evaluated by using MTT assay. Also, In order to demonstrate the effect of drugs on the apoptosis, a quantitative method real-time PCR was used. The interaction of plasma proteins was investigated by Magnevist conjugated dendrimer compared with Magnevist using SDS-PAGE technique to determine the fate of nanoparticles. Result of study revealed that it quite significantly affects Bax and increasing apoptosis. Its effect on Bcl2 is more than net gadopentetic acid and almost equivalent to the effect of dendrimer alone. In fact, a significant increase was observed in Bax compared to the control group, and no significant effect was observed on BCL2 gene. Also, corona protein was investigated and the protein with weight less than 70 kDa was observed around a nanoparticle that seems albumin protein with a molecular weight of 67 kDa. Conducted study revealed the acceptable therapeutic effect of Magnevist conjugated dendrimer and it was comparable with in vitro Magnevist model of breast cancer. Therefore, the use of the denderimer can be effective on increasing expression of some proapoptoticgenes and provided a new perspective in clinical diagnostic.