Effect of Maleic Acid Root Conditioning on Release of Transforming Growth Factor Beta 1 from Infected Root Canal Dentin

Ballal, Vasudev; Narkedamalli, Raj Kumar; Ruparel, Nikita B.; Shenoy, Padmaja Ananth; Bhat, Vinutha; Belle, Vijetha Shenoy

doi:10.1016/j.joen.2022.02.007

Cited by 9 publications

(7 citation statements)

References 25 publications

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“…However, the presence of bio lm may alter the amount of GF released, which may affect regenerative potential. Studies have shown that the presence of bio lm on the dentin surface reduces GF release (29,38). Many studies evaluating the effect of different chelation agents and IATs have measured the release of GFs from healthy dentin to simulate clinical conditions (6,8,14,19,20).…”

Section: Discussionmentioning

confidence: 99%

Comparative Effects of 17% Ethylenediaminetetraacetic Acid and 9% Etidronic Acid Applied with Different Irrigant Activation Techniques on the Release of Growth Factors from Dentin: In Vitro Study

Mumcu,

Kurnaz,

Kiraz

et al. 2024

Preprint

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Background Growth factors embedded in the extracellular matrix of the dentin play an important role in the migration, proliferation, and differentiation of dental pulp stem cells in regenerative endodontics. In regenerative endodontic treatments, the type of irrigation solution used is crucial for the release of growth factors (GFs) from the dentin matrix. This study evaluated the effectiveness of different irrigant activation techniques (IAT) using two different chelating agents, 17% ethylenediaminetetraacetic acid (EDTA) and 9% etidronic acid (HEDP), in terms of their GF release.Methods Seventy-two mandibular premolar teeth were prepared to simulate an open apex. The root fragments were irrigated with 20 ml of 1.5% sodium hypochlorite and 20 ml of saline solution. Eight root fragments were randomly separated for the control group, and the remaining 64 fragments were randomly separated into eight groups based on two different chelating agents (17% EDTA and 9% HEDP) and four different IAT (conventional needle irrigation (CNI), passive ultrasonic irrigation (PUI), sonic activation with EDDY, and XP-endo Finisher (XPF)). TGF-β1, VEGF-A, BMP-7 and IGF-1 release levels were determined using an ELISA, and statistical analysis was performed using the Kolmogorov–Smirnov test, ANOVA, and the Tukey test (p < .05).Results Compared to the control group, the experimental groups showed significantly higher GF release when using EDTA or HEDP. Among the activation groups, the EDDY group triggered the highest GF release, and the CNI group triggered the lowest.Conclusions IAT with EDTA and HEDP can increase GF release, with EDDY being the most effective IAT method. Using chelating agents with IAT may be beneficial in regenerative endodontic treatments.

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Section: Discussionmentioning

confidence: 99%

Comparative Effects of 17% Ethylenediaminetetraacetic Acid and 9% Etidronic Acid Applied with Different Irrigant Activation Techniques on the Release of Growth Factors from Dentin: In Vitro Study

Mumcu,

Kurnaz,

Kiraz

et al. 2024

Preprint

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“…Such models are critical to be employed since it has been shown that the structure of dentin previously exposed to bacterial biofilms is altered which may negatively affect the migration, attachment, proliferation and differentiation of recruited stem and progenitor cells. Furthermore, infected dentin may sequester variable levels of growth factors as compared to natural healthy dentin [ 76 ]. This is in addition to the fact that it has been shown that the larger the periapical lesion and the longer the infection, the higher the virulence of microorganisms thereby creating a more challenging environment for tissue regeneration [ 77 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of extracellular matrix scaffolds on histological outcomes of regenerative endodontics in experimental animal models: a systematic review

Elnawam,

Abdallah,

Nouh

et al. 2024

BMC Oral Health

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Background Decellularized extracellular matrix (dECM) from several tissue sources has been proposed as a promising alternative to conventional scaffolds used in regenerative endodontic procedures (REPs). This systematic review aimed to evaluate the histological outcomes of studies utilizing dECM-derived scaffolds for REPs and to analyse the contributing factors that might influence the nature of regenerated tissues. Methods The PRISMA 2020 guidelines were used. A search of articles published until April 2024 was conducted in Google Scholar, Scopus, PubMed and Web of Science databases. Additional records were manually searched in major endodontic journals. Original articles including histological results of dECM in REPs and in-vivo studies were included while reviews, in-vitro studies and clinical trials were excluded. The quality assessment of the included studies was analysed using the ARRIVE guidelines. Risk of Bias assessment was done using the (SYRCLE) risk of bias tool. Results Out of the 387 studies obtained, 17 studies were included for analysis. In most studies, when used as scaffolds with or without exogenous cells, dECM showed the potential to enhance angiogenesis, dentinogenesis and to regenerate pulp-like and dentin-like tissues. However, the included studies showed heterogeneity of decellularization methods, animal models, scaffold source, form and delivery, as well as high risk of bias and average quality of evidence. Discussion Decellularized ECM-derived scaffolds could offer a potential off-the-shelf scaffold for dentin-pulp regeneration in REPs. However, due to the methodological heterogeneity and the average quality of the studies included in this review, the overall effectiveness of decellularized ECM-derived scaffolds is still unclear. More standardized preclinical research is needed as well as well-constructed clinical trials to prove the efficacy of these scaffolds for clinical translation. Other The protocol was registered in PROSPERO database #CRD42023433026. This review was funded by the Science, Technology and Innovation Funding Authority (STDF) under grant number (44426).

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“…The EDTA conditioning of root canal dentin supports the cell survival, attachment, and differentiation of DPSCs and GF release from the dentin matrix [ 26 , 28 ]. Various chemical substances, such as phosphoric acid, citric acid, phytic acid, and maleic acid, have been tested for their effectiveness in releasing GFs in regenerative endodontics [ 6 , 14 , 19 , 29 ]. Although HEDP has been recommended for clinical use due to its chelating properties, research on its use as a final irrigation agent to release GFs in regenerative endodontics is limited [ 13 , 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, the presence of biofilm may alter the amount of GF released, which may affect regenerative potential. Studies have shown that the presence of biofilm on the dentin surface reduces GF release [ 29 , 38 ]. Many studies evaluating the effect of different chelation agents and IATs have measured the release of GFs from healthy dentin to simulate clinical conditions [ 6 , 8 , 14 , 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative effects of 17% ethylenediaminetetraacetic acid and 9% etidronic acid applied with different irrigant activation techniques on the release of growth factors from dentin: in vitro study

Mumcu,

Kurnaz,

Kiraz

et al. 2024

BMC Oral Health

View full text Add to dashboard Cite

Background Growth factors embedded in the extracellular matrix of the dentin play an important role in the migration, proliferation, and differentiation of dental pulp stem cells in regenerative endodontics. In regenerative endodontic treatments, the type of irrigation solution used is crucial for the release of growth factors (GFs) from the dentin matrix. This study evaluated the effectiveness of different irrigant activation techniques (IAT) using two different chelating agents, 17% ethylenediaminetetraacetic acid (EDTA) and 9% etidronic acid (HEDP), in terms of their GF release. Methods Seventy-two mandibular premolar teeth were prepared to simulate an open apex. The root fragments were irrigated with 20 ml of 1.5% sodium hypochlorite and 20 ml of saline solution. Eight root fragments were randomly separated for the control group, and the remaining 64 fragments were randomly separated into eight groups based on two different chelating agents (17% EDTA and 9% HEDP) and four different IAT ((conventional needle irrigation (CNI), passive ultrasonic irrigation (PUI), sonic activation with EDDY, and XP-endo Finisher (XPF)). TGF-β1, VEGF-A, BMP-7 and IGF-1 release levels were determined using an ELISA, and statistical analysis was performed using the Kolmogorov–Smirnov test, ANOVA, and the Tukey test (p < .05). Results Compared to the control group, the experimental groups showed significantly higher GF release when using EDTA or HEDP. Among the activation groups, the EDDY group triggered the highest GF release, and the CNI group triggered the lowest. Conclusions IAT with EDTA and HEDP can increase GF release, with EDDY being the most effective IAT method. Using chelating agents with IAT may be beneficial in regenerative endodontic treatments.

show abstract

Effect of Maleic Acid Root Conditioning on Release of Transforming Growth Factor Beta 1 from Infected Root Canal Dentin

Cited by 9 publications

References 25 publications

Comparative Effects of 17% Ethylenediaminetetraacetic Acid and 9% Etidronic Acid Applied with Different Irrigant Activation Techniques on the Release of Growth Factors from Dentin: In Vitro Study

Comparative Effects of 17% Ethylenediaminetetraacetic Acid and 9% Etidronic Acid Applied with Different Irrigant Activation Techniques on the Release of Growth Factors from Dentin: In Vitro Study

Influence of extracellular matrix scaffolds on histological outcomes of regenerative endodontics in experimental animal models: a systematic review

Comparative effects of 17% ethylenediaminetetraacetic acid and 9% etidronic acid applied with different irrigant activation techniques on the release of growth factors from dentin: in vitro study

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