The objective of this study was to investigate the effects of different growing media on plant growth and nutrient contents of petunia (Petunia hybrida). The experiment was conducted in a chamber room under controlled conditions at the laboratory. Seven different media (soil, 3:1 soil: peat (P1), 2:1 soil: peat (P2), 3:1 soil: barnyard manure (BYM1), 2:1 soil: barnyard manure (BYM2), 3:1 soil: sugar beet pulp (SBP1), 2:1 soil: sugar beet pulp (SBP2)) were used as plant growing media. The experiment was ended after three months following transplanting of seedlings. Plant growth and flower parameters and macro-micro nutrient contents were determined in harvested plants. The highest stem diameter, branch number, flower diameter, flower stalk diameter, plant fresh weight and plant dry weight were in 2:1 ratio of soil: peat mixture as 7.00 mm, 6.33, 8.91 cm, 3.59 mm, 48.47 g and 4.52 g while the highest plant lenght, lateral branch number and flower number means were found as 27.43 cm, 24.67 and 24.67 in ratio of 3:1 soil: peat growing media respectively. The highest potassium, magnesium and zinc means of plants were obtained as 6.6%, 2.2% and 32.50 mg kg-1 in soil: barnyard manure (2:1) growing media while the highest phosphorus and calcium means of plants were found as 0.27% and 4.5% in soil and peat growing media respectively. The highest iron and copper means of plants were determined as 231 mg kg-1 and 32.50 mg kg-1 in ratios of 2:1 and 3:1 of soil: sugarbeet pulp growing media respectively.