Effect of Marine Basidiomycetes Fulvifomes sp.-Derived Ergosterol Peroxide on Cytotoxicity and Apoptosis Induction in MCF-7 Cell Line

Govindharaj, Mano; Arumugam, Sathishkumar; Nirmala, Grace; Bharadwaj, Mausumi; Murugiyan, Kalaiselvam

doi:10.3390/jof5010016

Cited by 11 publications

(5 citation statements)

References 23 publications

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“…Homolytic cleavage of the peroxide moiety in a reducing medium leads to the formation of reactive oxygen species (ROS), which are powerful internal stimuli for apoptosis. This has been confirmed, in particular, in experiments with MCF-7 cells [ 124 ]. Their treatment with 21a at concentrations of 40–80 μg/mL led to an increase in the production of ROS in a dose-dependent manner and to the induction of apoptosis.…”

Section: Endoperoxidessupporting

confidence: 57%

Structure and Biological Activity of Ergostane-Type Steroids from Fungi

2022

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Mushrooms are known not only for their taste but also for beneficial effects on health attributed to plethora of constituents. All mushrooms belong to the kingdom of fungi, which also includes yeasts and molds. Each year, hundreds of new metabolites of the main fungal sterol, ergosterol, are isolated from fungal sources. As a rule, further testing is carried out for their biological effects, and many of the isolated compounds exhibit one or another activity. This study aims to review recent literature (mainly over the past 10 years, selected older works are discussed for consistency purposes) on the structures and bioactivities of fungal metabolites of ergosterol. The review is not exhaustive in its coverage of structures found in fungi. Rather, it focuses solely on discussing compounds that have shown some biological activity with potential pharmacological utility.

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Section: Endoperoxidessupporting

confidence: 57%

Structure and Biological Activity of Ergostane-Type Steroids from Fungi

2022

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“…The cells were fixed for 30 minutes at room temperature in a 3:1 combination of methanol and glacial acetic acid, washed in PBS, and then stained with AO/ EtBr in a 1:1 ratio. The stained cells were rinsed in PBS and inspected at a magnification of 40x using a fluorescence microscope (16).…”

Section: Apoptosis Assaymentioning

confidence: 99%

In-vitro Antioxidant and Cytotoxic Effects of Physalis minima Linn. in HeLa Cell Lines Against Cervical Cancer

Velemurugan,

Sethuraman,

Kamaraj

2024

Curr. Trends Biotechnol. Pharm.

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In the present study, the shade-dried and powdered leaves of Physalis minima Linn. were subjected to Soxhlet extraction with methanol, concentrated, and further partitioned with n-hexane, chloroform, and ethanol. The in-vitro anti-oxidant activity was evaluated by DPPH radical scavenging assay and the cytotoxic potential by MTT assay in HeLa cell lines. The results of the study revealed that among the three fractions, the chloroform fraction of the methanolic extract showed better antioxidant activity (IC 50 = 38.6 µg/mL) compared to standard Ascorbic acid (IC 50 = 42.6 µg/mL). Moreover, the results of the MTT assay carried out in HeLa cell lines showed that the chloroform fraction exhibited a promising cytotoxic activity (IC 50 = 59.66 µg/mL). Further, the chloroform fraction when subjected to apoptosis assay using acridine orange -ethidium bromide dual staining displayed the morphological characteristics of apoptotic event including nuclear fragmentation and chromatin condensation with orange red and reddish nuclei. The results of our study support the protective effect of chloroform fraction of methanolic extract of leaves of Physalis minima Linn., in the treatment of cervical cancer.

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“…MTT assays that utilize the reduction of tetrazolium salt are now widely accepted as a reliable way to examine cell proliferation [32][33][34][35]. We used MTT assay in our experimental studies to evaluate the response of cells after treatment with external factors such as UV light (385 nm), and photochromic molecule of Azobenzene [36]. Yellow-colored MTT is reduced to purple formazan in the mitochondria of living cells.…”

Section: Mtt Cell Proliferation Assaymentioning

confidence: 99%

On-chip mixing of cancer cells and drug using LED enabled 2D opto-wetting droplet platforms

Thomas

Govindharaj

Unni

et al. 2023

Biomed. Phys. Eng. Express

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Droplets of microliter size serve as miniaturized reaction chambers for practical lab on a chip (LoC) applications. The transportation and coalescence of droplets are indispensable for realizing microfluidic mixing. Light can be used as an effective tool for droplet manipulation. We report a novel platform for LED-based transport and mixing of cell-encapsulated microdroplets for evaluating dose response of cancer drugs. Microcontroller enabled LEDs (Light-emitting diodes) were used to actuate droplet movement on Azobenzene coated planar silicon substrates. Droplet transport was initiated by the spatial gradient in solid-liquid interfacial tension developed through LED triggered photoisomerization of Azobenzene substrate. Detailed UV-Visible characterization of Azobenzene molecule was performed for different LED light intensity and wavelength. A complete standalone opto-wetting toolbox was developed by integrating various components such as a microcontroller, UV LED (385nm), blue LED (465nm), and Azobenzene coated photoresponsive substrate. 2D transport of DI water droplets (10 -30 μL) along simple trajectories was demonstrated using this device. Subsequently, the proposed opto-wetting platform was used for performing drug evaluation through on-chip mixing of droplets containing cancer cells (A549 – Lung cancer cells) and cancer drug (paclitaxel). Separate cell viability analysis was performed using MTT assays, where the cytotoxic effects of Azobenzene and UV light (385nm) on A549 cells were studied. Dosage response of paclitaxel drug was studied using both MTT (3-(4, 5-Dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) and live-dead cell assays. The results obtained indicate the potential use of our device as a cost-effective, reliable opto-wetting microfluidic platform for drug screening experiments.

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Effect of Marine Basidiomycetes Fulvifomes sp.-Derived Ergosterol Peroxide on Cytotoxicity and Apoptosis Induction in MCF-7 Cell Line

Cited by 11 publications

References 23 publications

Structure and Biological Activity of Ergostane-Type Steroids from Fungi

Structure and Biological Activity of Ergostane-Type Steroids from Fungi

In-vitro Antioxidant and Cytotoxic Effects of Physalis minima Linn. in HeLa Cell Lines Against Cervical Cancer

On-chip mixing of cancer cells and drug using LED enabled 2D opto-wetting droplet platforms

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