Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain

Wang, Qian; Lin, Jing; Yang, Peng; Liang, Yingye; Lu, Dongming; Wang, Kailong; Gan, Wei; Fu, Jianping; Gan, Zhenbao; Ma, Mingchen; Wu, Pingting; He, Fengshi; Pang, Jun; Tang, Hongliang

doi:10.1155/2020/8309745

Cited by 12 publications

(9 citation statements)

References 13 publications

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“…The high levels of inflammatory cytokines in elderly individuals [28] are considered to be caused by underlying inflammatory diseases. Recent findings suggest that a reduction in the release of inflammatory cytokines via the inhibition of the TLR4 signaling pathway may be a mechanism by which massage can relieve neuropathic pain [48]. Atanackovic et al [40] demonstrated a marked but short-term increase in IL-6 and TNFα levels in patients with malignant diseases during the first 5-24 h after whole-body hyperthermia.…”

Section: Discussionmentioning

confidence: 99%

Modulation of the human immune status by spinal thermal massage: a non-randomized controlled study

2021

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Thermal and massage therapies have long been used to control pain. Although spinal thermal massage (STM) has been used worldwide, its effectiveness has not been proven in a controlled clinical study. We here conducted a non-randomized controlled trial to assess the pain-relieving and immunomodulatory effects of STM in old-aged patients experiencing pain or disability. The experimental group was treated with STM five times a week for 8 weeks and rehabilitative regular care (RRC). The control group was treated with only RRC. Pain and immunological parameters were tested before treatment and after 4 and 8 weeks of treatment. The scores of three pain parameters were lowered by STM, and the differences between the groups were statistically significant at the two time points (p < 0.01). Quality of life determined using the 3-level EuroQol five-dimensional questionnaire scores was significantly higher in patients in the experimental group than those in the control group. Effect sizes (ES) were in the range of medium to large in the pain-related measures (0.54–1.22). The total leukocyte counts and the proportions of lymphocytes and subsets were not significantly different between the groups, whereas the proportions of monocytes and natural killer (NK) cells were higher in the experimental group than in the control group after 8 weeks (p < 0.05). The production of interleukin (IL)-4 and interferon γ in T cells was not significantly different between the groups, whereas the production of IL-2 was high in the control group. However, there was a significant increase in IFN-γ production by NK cells in the experimental group (at 4 weeks, p < 0.05). ES were medium in the immunological measures (0.53–0.68). No significant difference was observed in the production of proinflammatory cytokines, IL-1β, tumor necrosis factor α, or IL-6 between the groups. In conclusion, STM treatment has a positive effect on subjective pain and quality of life. It also enhanced NK cell proportion and activity, suggesting that STM may be beneficial in the prevention of viral diseases and cancer in old-aged people.

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Section: Discussionmentioning

confidence: 99%

Modulation of the human immune status by spinal thermal massage: a non-randomized controlled study

2021

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“…If a rat lifted or licked its paw three or more times under the filament stimulation, the result was regarded as positive; otherwise, it was deemed negative. The force that triggered a positive reaction was considered the PWMT (He et al., 2010; Wang et al., 2020). The test was finished when a positive reaction was observed.…”

Section: Methodsmentioning

confidence: 99%

Propofol alleviates neuropathic pain in chronic constriction injury rat models via the microRNA‐140‐3p/Jagged‐1 peptide/Notch signaling pathway

Fang

Yang

Yan

et al. 2021

Synapse

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Chronic constriction injury (CCI) of the sciatic nerve was used to establish neuropathic pain (NP) models in rats. CCI rats were then treated with propofol (Pro) and their paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured. In addition, the expression patterns of tumor necrosis factor‐α (TNF‐α), interleukin‐1β (IL‐1β), and IL‐10 were detected. CCI rats treated with propofol were further injected with antagomiR‐140‐3p to verify the role of miR‐140‐3p in propofol's analgesic actions. In addition to confirming the relationship between miR‐140‐3p and JAG1, the expression patterns of JAG1 itself were detected. Propofol‐treated CCI rats were also injected with Ad‐JAG1 (adenovirus‐packaged JAG1 overexpression vector and Ad‐NC) to test the role of JAG1 in propofol's analgesic mechanism of action. Finally, the levels of JAG1 and Notch pathway‐related proteins were detected Results Propofol was found to alleviate NP, including thermal hyperalgesia and mechanical pain threshold. Propofol could also ameliorate neuroinflammation by up‐regulating the expression of IL‐10 and inhibiting the release of TNF‐α and IL‐1β. Mechanically, propofol enhanced the amount of miR‐140‐3p in CCI rats via the regulation of JAG1. Down‐regulation of miR‐140‐3p, or up‐regulation of JAG1, could reduce the protective effect of propofol against NP. Propofol inhibited the activation of Notch signaling via miR‐140‐3p/JAG1 to realize its analgesic effect Conclusion Our findings indicated that propofol inhibits inflammatory responses and the Notch signaling pathway via miR‐140‐3p/JAG1 to alleviate NP. These data provide evidence to support a potential clinical therapy for NP.

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“…If a rat lifted or licked its paw 3 or more times under the lament stimulation, the result was regarded as positive; otherwise, it was negative. The force that triggered a positive reaction was called PWMT (He et al 2010;Wang et al 2020). The test was nished when a positive reaction was observed.…”

Section: Pwmtmentioning

confidence: 99%

Propofol Alleviates Neuropathic Pain in CCI Rat Model via the miR-140-3p/JAG1/Notch Signaling Pathway

Fang

Yang

Yan

et al. 2021

Preprint

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As a renowned anesthetic, propofol exerts excellent analgesic function in nerve injury. However, the underlying mechanism of propofol on neuropathic pain (NP) remains unknown. The research aims to analyze propofol’s analgesia mechanism to alleviate NP in CCI rats. The chronic constriction injury (CCI) of sciatic nerve was used to established NP rat models. CCI rats were treated with propofol and its paw withdrawal mechanical threshold (PMWT) and paw withdraw thermal latency (PWTL) were measured. The expressions of TNF-α, IL-1β and IL-10 were detected. CCI rats with propofol treatment were injected with antagomiR-140-3p. After the targeting relationship between miR-140-3p and JAG1 was checked, JAG1 expression was detected. Propofol-treated CCI rats were further injected with Ad-JAG1. Finally, the levels of JAG1 and Notch pathway-related proteins were detected. As a result, propofol could alleviate NP, including thermal hyperalgesia and mechanical pain threshold, and ameliorate neuroinflammation. Mechanically, propofol enhanced the level of miR-140-3p in CCI rats. JAG1 was a direct target of miR-140-3p. The downregulation of miR-140-3p or upregulation of JAG1 could reduce the protective effect of propofol against NP. Propofol inhibited activation of Notch signaling via miR-140-3p/JAG1. Overall, Propofol could inhibit the neuroinflammation and Notch signaling pathway via miR-140-3p/JAG1 to alleviate NP.

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Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain

Cited by 12 publications

References 13 publications

Modulation of the human immune status by spinal thermal massage: a non-randomized controlled study

Modulation of the human immune status by spinal thermal massage: a non-randomized controlled study

Propofol alleviates neuropathic pain in chronic constriction injury rat models via the microRNA‐140‐3p/Jagged‐1 peptide/Notch signaling pathway

Propofol Alleviates Neuropathic Pain in CCI Rat Model via the miR-140-3p/JAG1/Notch Signaling Pathway

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