Effect of Matrix Clean-Up for Aflatoxin Analysis in Corn and Dried Distillers Grains

Mcdaniel, Ah; Holmes, William E.; Armbrust, Kevin L.; Sparks, Darrell; Brown, A. C.

doi:10.4236/nr.2011.24032

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…These analytical methods are laborious, expensive with requirement of sophisticated equipment, skilled personnel and complicated sample preparation processes that limit their application to laboratories (Köppen et al, 2010; McDaniel et al, 2011). Molecular techniques like PCR, RT-PCR are available for the detection of aflatoxigenic fungi but matrix associated inhibitors in the samples may lead to inaccurate results (Levin, 2012).…”

Section: Introductionmentioning

confidence: 99%

Immuno Affinity SELEX for Simple, Rapid, and Cost-Effective Aptamer Enrichment and Identification against Aflatoxin B1

et al. 2016

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Aflatoxins are naturally occurring mycotoxins that contaminate food and agro commodities, leading to acute and chronic health conditions in human and animals. In the present work, an attempt was made to generate high-affinity single stranded DNA aptamers that specifically bind to Aflatoxin B1 (AFB1) by a modified Systemic Evolution of Ligands by Exponential Enrichment (SELEX) procedure with the aid of Immunoaffinity columns. Ten rounds of SELEX and alternating three counter SELEX rounds with a cocktail of related and other mycotoxins were performed to enhance the specificity. Resultant 105 aptamers were clustered into 12 groups according to their primary sequence homology. Candidates with lowest Gibbs free energy (dG value) and unique stem loop structures were selected for further characterization. Aptamers, AFLA5, AFLA53, and AFLA71 exhibiting lower Kd values (50.45 ± 11.06, 48.29 ± 9.45, and 85.02 ± 25.74 nM) were chosen for development of ELONA and determination of purification ability of toxin. The detection limit (LOD) of AFLA5 and AFLA71 was 20 and 40 ng/ml, respectively. HPLC analysis implied that selected aptamers were able to recover and quantify 82.2 to 96.21% (LOQ – 53.74 ng) and 78.3 to 94.22% (LOQ – 66.75 ng) of AFB1 from spiked corn samples, respectively. These findings indicate, immunoaffinity based SELEX can pave an alternative approach to screen aptamers against mycotoxin detection and purification.

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Section: Introductionmentioning

confidence: 99%

Immuno Affinity SELEX for Simple, Rapid, and Cost-Effective Aptamer Enrichment and Identification against Aflatoxin B1

et al. 2016

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“…Aflatoxin [13][14] B1 is the most toxic and frequently detected form. The level of aflatoxin increases as the colonies of these causative fungi also increase.…”

Section: Aflatoxin Levelsmentioning

confidence: 99%

Packaging and Shelf Life of Maize-Peanut Balls (Dakua)

JK¹

2017

MOJFPT

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Maize-peanut balls (also known as dakua, dzowe or donkwa) is a street snack made from roasted, dried and milled maize and groundnuts/peanuts. It is often sold unpackaged by hawkers/vendors, in a portable food booth, food cart or food truck. However, the shelf life is unknown which makes this nutritious snack also a potential risk to consumers' health. In this study, the packaging and shelf life of maize-peanut balls (lab/self-made and those purchased from the local markets) were investigated by packaging in aluminum foil, flexible plastic films and unpackaged and stored at ambient temperature for 5 weeks. The products were analysed weekly for moisture, peroxide value, aflatoxin level, yeast and moulds during storage. Results indicate that the samples had variable moisture content, which decreased from the start of the analysis to the third week and increased again. Over the 5 weeks storage period, peroxide values increased to ~0.4 mEq/ kg. The unpackaged samples had the highest aflatoxin level (~21.74 ppb), which is above the acceptable limit of 20 ppb. The samples packaged in flexible plastic film, however, had the lowest aflatoxin level (~13.15 ppb) whereas samples packaged in aluminum foil had values of ~16.75 ppb. The estimated shelf life is approximately 8 weeks with flexible plastic films as the most suitable packaging material.

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Effect of Solvent on Aflatoxin Content in Extracts of Virginia Type Peanut Skins

Sarnoski

2015

J Food Health Sci

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Abstract:Antimicrobial resistance of pathogenic bacteria is a growing public health problem. Methicillin-resistant Staphylococcus aureus (MRSA) represents a significant burden on healthcare services because it is involved in severe difficult to treat infections in humans. Several non-antibiotic approaches regarding the treatment of infections caused by MRSA as well as of other resistant bacteria have been studied. Since ancient times royal jelly and honey have been considered both as foods and medicines, and recently have been identified as potential alternative medicines to treat bacterial infections, especially of the skin and soft tissue. The aim of the present study was to investigate the antibacterial effect of honey, royal jelly and their mixtures against MRSA in vitro. At least 3 decimal reductions of MRSA count were observed in Tryptone Soy broth with concentrations of 40% rape honey (RP), 20% and 30% royal jelly (RJ), 30% RJ:RH (1:100). In general, honey and royal jelly, individually or in combination, appeared to have a potential as alternative therapeutic agents against MRSA infections, but clinical studies are needed for confirmation.

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Effect of Matrix Clean-Up for Aflatoxin Analysis in Corn and Dried Distillers Grains

Cited by 3 publications

References 28 publications

Immuno Affinity SELEX for Simple, Rapid, and Cost-Effective Aptamer Enrichment and Identification against Aflatoxin B1

Immuno Affinity SELEX for Simple, Rapid, and Cost-Effective Aptamer Enrichment and Identification against Aflatoxin B1

Packaging and Shelf Life of Maize-Peanut Balls (Dakua)

Effect of Solvent on Aflatoxin Content in Extracts of Virginia Type Peanut Skins

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