Effect of maturation media and oocytes derived from sows or gilts on the development of cloned pig embryos

Hyun, Sang Hwan; Lee, G.S; Kim, D.Y; Kim, H.S; Lee, S.H; Kim, S; Lee, E.S; Lim, Jeeyoung; Kang, Sa-Ouk; Lee, B.C; Hwang, Wontae

doi:10.1016/s0093-691x(02)01211-6

Cited by 56 publications

(32 citation statements)

References 24 publications

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“…In this present study we found that canine intergeneric nuclear transfer using pigs and cows oocytes as recipient oocytes have no different result in fusion condition and this result almost same with result used in porcine cloning around 68-75% (Hao et al, 2003;Hyun et al, 2003). A majority of the fused embryos in this study initiated cleavage and developed to the 16 cell stage of development.…”

Section: Discussionsupporting

confidence: 71%

Development of Intergeneric Canine Embryo Using Bovine and Porcine Oocyte as Cytoplasm Recipient

Fibrianto

2015

Indones. J. Biotechnol.

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This study was conducted to increase the efficiency of canine embryo production by intergeneric somatic cell nuclear transfer (SCNT) technology. The effect of oocyte recipient for development of intergeneric canine somatic cell cloning embryos were examined. Bovine and porcine cumulus oocyte complexes (COCs) were collected from slaughterhouse ovaries and matured in TCM-199 medium depend on species. Adult dog fibroblasts collected from 3.5 years old Afghanhound dog, and cell between passage 1 and passage 10 used for intergeneric somatic cell cloning using bovine and porcine oocytes as oocyte cytoplasm donor. The result showed that oocytes from bovine and porcine can de-differentiated canine nucleus and no different between bovine and porcine oocyte in fusion and embryo development in vitro. Canine intergeneric cloned embryos developed to morula stages in vitro.

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Section: Discussionsupporting

confidence: 71%

Development of Intergeneric Canine Embryo Using Bovine and Porcine Oocyte as Cytoplasm Recipient

Fibrianto

2015

Indones. J. Biotechnol.

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“…Previous studies in swine [34], caprine [35] and bovine [17] also found that BCB+ oocytes were larger than BCB− oocytes. Many authors studying the relationship between oocyte diameter and developmental potency have concluded that the developmental potential of oocytes increases with increasing oocyte diameters [12,[56][57][58][59][60]. Furthermore, it has been demonstrated that mouse oocytes with SN configurations were larger, more advanced toward ovulation and more competent in development than oocytes with NSN pattern [30,31,43,61].…”

Section: Discussionmentioning

confidence: 99%

Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: a study using the mouse model

Liu

Zhou

et al. 2007

Cell Res

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“…O'Brien et al [11] reported that the mitochondria and cortical granules in adult sheep oocytes were larger than those in prepubertal sheep oocytes, and the rate of developmental to the blastcyst stage was lower in prepubertal sheep oocytes than in adult sheep oocytes. Their findings were likely caused by the different characteristics in the cytoplasm of prepubertal and adult sheep oocytes [23]. Nevertheless, Asada et al [4] found no differences in the proportions of maturation, penetration and pronuclei formation between prepubertal and adult minke whale oocytes.…”

Section: Discussionmentioning

confidence: 92%

Comparative Studies on Lipid Analysis and Ultrastructure in Porcine and Southern Minke Whale (Balaenoptera Bonaerensis) Oocytes

Fujihira

Kinoshita

Sasaki

et al. 2004

Journal of Reproduction and Development

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Abstract. The present study was conducted to clarify the difference in the color of the cytoplasm in immature follicular oocytes from prepubertal and adult minke whales. The four lipid contents (triglyceride, total cholesterol, phospholipids and non-esterified fatty acids) in vitrified immature oocytes from prepubertal and adult minke whales, and also in fresh and vitrified immature porcine oocytes, were measured. The lipid contents in vitrified-warmed minke whale oocytes were similarly high compared with those in vitrified-warmed porcine oocytes. In particular, the total cholesterol and phospholipid contents in the vitrified immature oocytes from prepubertal and adult minke whales were significantly (P<0.05) higher than those from prepubertal pigs. Furthermore, the distribution of lipid droplets in fresh and vitrified immature oocytes was observed in transmission electron microscopy. Lipid droplets in the prepubertal minke whale oocytes were distributed throughout the cytoplasm. In contrast, adult minke whales had larger lipid droplets which were distributed mainly in the central portion of the cytoplasm. The lipid droplets of immature oocytes from prepubertal pigs were larger than those in minke whale oocytes. These results indicated that the difference in the distribution of the cytoplasmic lipid droplets may result in the difference in the color tone of both prepubertal and adult whale oocyte cytoplasm. Key words: Minke whale, Porcine, Oocytes, Lipid, Sexual maturity (J. Reprod. Dev. 50: [525][526][527][528][529][530][531][532] 2004) e have been studying in vitro maturation (IVM), fertilization (IVF) and culture (IVC) in the artificial production of minke whale embryos [1][2][3][4]. During the studies, we observed a difference in the color of the cytoplasm in immature follicular oocytes from prepubertal and adult minke whales. The cytoplasm of the oocytes collected from prepubertal whales was opaque and dark, whereas the oocytes from adult whales had bright and transparent cytoplasm (Fig. 1). It was considered that the difference in the color of the two oocyte types was related to the lipid content in the cytoplasm. Lipids in the oocytes are mainly involved in energy storage, constitute the c y t o p l a s m i c m e m b r a n e a n d c y t o p l a s m i c organelles, and adjust the physiological function. Lipid analyses in oocytes or embryos have been conducted in domestic animals [5][6][7][8], and it was reported that porcine oocytes had a dark color tone of the cytoplasm as well as minke whale oocytes, and porcine oocytes had higher lipid content than the oocytes of other species [7].Follicular oocytes of many species are very

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Effect of maturation media and oocytes derived from sows or gilts on the development of cloned pig embryos

Cited by 56 publications

References 24 publications

Development of Intergeneric Canine Embryo Using Bovine and Porcine Oocyte as Cytoplasm Recipient

Development of Intergeneric Canine Embryo Using Bovine and Porcine Oocyte as Cytoplasm Recipient

Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: a study using the mouse model

Comparative Studies on Lipid Analysis and Ultrastructure in Porcine and Southern Minke Whale (Balaenoptera Bonaerensis) Oocytes

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