Effect of Mechanical Stretching on Expressions of Muscle Specific Transcription Factors MyoD, Myf‐5, Myogenin and MRF4 in Proliferated Myoblasts

Abe, Shinichi; Rhee, Sunki; Iwanuma, Osamu; Hiroki, Emi; Yanagisawa, Nobuaki; Sakiyama, Kazuo; Ide, Yoshinobu

doi:10.1111/j.1439-0264.2009.00945.x

Cited by 24 publications

(18 citation statements)

References 21 publications

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“…Similarly, in the present study, we identified significant (P < 0.05) and positive correlation between the expression of DLK1 and IGF-I. Abe et al (2009) indicated that IGF-I expression showed the same trend as that of MyHC 2b from prenatal days 16-18. The increase in MyHC 2b expression enlarged the muscle fiber diameter; thus, IGF-I may be a regulatory factor associated with the growth of muscle and muscle fiber diameter enlargement.…”

Section: Discussionsupporting

confidence: 60%

Association between DLK1 and IGF-I gene expression and meat quality in sheep

Su¹,

Sun²,

Li³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The aim of the present study was to detect delta-like 1 homolog (DLK1) and insulin-like growth factor-I (IGF-I) gene expression in the longissimus dorsi of Hu sheep at different growth stages and study the association between these genes and meat quality. The diameter and density of muscle fibers and tenderness of the longissimus dorsi were measured. Growth stage, but not sex, significantly affected DLK1 and IGF-I expression. DLK1 and IGF-I expression in the sheep longissimus dorsi gradually increased with growth, but also decreased during some periods. These results suggest that different growth stages significantly affect DLK1 and IGF-I gene expression in sheep muscle tissue. The expression of DLK1 and IGF-I genes were positively and significantly (P < 0.01) correlated with muscle fiber diameter and muscle fiber shear stress, and negatively and significantly (P < 0.01) correlated with muscle fiber density. Muscle fiber diameter was positively and significantly (P < 0.01) correlated with muscle fiber shear stress, and negatively and significantly (P < 0.01) correlated with muscle fiber density. In addition,

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Section: Discussionsupporting

confidence: 60%

Association between DLK1 and IGF-I gene expression and meat quality in sheep

Su¹,

Sun²,

Li³

et al. 2014

Genet. Mol. Res.

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“…22,23 In addition, they are able to rearrange themselves on the culture substrate, 24,25 which is consistent to the principle of actin fiber reorganization observed in many cell types. [26][27][28][29][30] Strikingly, however, neither the application of a uniform strain field in all directions, the equibiaxial CTS, [31][32][33][34][35][36] nor uniaxial CTS applied parallel to the long axis of cells 37 seem to support myogenesis. This experimental evidence has fueled the currently established concept of impairment of differentiation by mechanical stimulation of mammalian myoblastic precursors.…”

Section: Introductionmentioning

confidence: 99%

Uniaxial Cyclic Strain Drives Assembly and Differentiation of Skeletal Myocytes

Pennisi

Olesen

Zee

et al. 2011

Tissue Engineering Part A

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Ex vivo engineering of skeletal muscle represents an exciting new area of biotechnology. Although the ability of skeletal muscle cells to sense and respond to mechanical forces is well known, strategies based on the use of mechanical stimuli to optimize myogenesis in vitro remain limited. In this work, we describe a simple but powerful method based on uniaxial cyclic tensile strain (CTS) to induce assembly and differentiation of skeletal myocytes in vitro. Confluent mouse myoblastic precursors cultured on flexible-bottomed culture plates were subjected to either uniaxial or equibiaxial CTS. The uniaxial CTS protocol resulted in a highly aligned array of cross-striated fibers, with the major axis of most cells aligned perpendicularly to the axis of strain. In addition, a short period of myogenin activation and significant increase in the myotube/myoblast ratio and percentage of myosin-positive myotubes was found, indicating an enhanced cell differentiation. In contrast, cells under equibiaxial strain regimen had no clear orientation and displayed signs of membrane damage and impaired differentiation. These results, thus, demonstrate that the selection of a proper paradigm is a key element when discussing the relevance of mechanical stimulation for myogenesis in vitro. This study provides a rational framework to optimize engineering of functional skeletal muscle.

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“…In vitro experiments have given comparable results: the genes MyoD and myf-5 were up-regulated early in the stretching process (first 12 h), whereas myogenin and MRF-4 were up regulated after 24 h of stretching before returning to control levels thereafter (Abe et al 2009). Boonen et al (2010), however, have presented seemingly contradictory results for the effects of stretching on muscle maturation and regeneration.…”

Section: Molecular Regulation Of Satellite Cellsmentioning

confidence: 78%

Stretching skeletal muscle in vitro: does it replicate in vivo physiology?

et al. 2011

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Skeletal muscle is highly adaptable and responds to changes in loading through exercise or resistance training through a number of mechanisms resulting in increased muscle mass and changes in contractile phenotype. To further understand and study the molecular mechanisms underlying the adaptive response of muscle, a number of in vitro culture systems have been developed that utilise mechanical loading or stretching of the cultured muscle to recapitulate the adaptations observed in vivo. Here we review the use of such stretching regimes for engineered muscle constructs and assess how well these in vitro systems mimic in vivo muscle physiology and adaptation.

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Effect of Mechanical Stretching on Expressions of Muscle Specific Transcription Factors MyoD, Myf‐5, Myogenin and MRF4 in Proliferated Myoblasts

Cited by 24 publications

References 21 publications

Association between DLK1 and IGF-I gene expression and meat quality in sheep

Association between DLK1 and IGF-I gene expression and meat quality in sheep

Uniaxial Cyclic Strain Drives Assembly and Differentiation of Skeletal Myocytes

Stretching skeletal muscle in vitro: does it replicate in vivo physiology?

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