Effect of Media Additives on the Production of Beauveria brongniartii, an Entomopathogenic Fungus of Holotrichia serrata

Srikanth, J.; Santhalakshmi, G.

doi:10.1007/s12355-012-0152-2

Cited by 9 publications

(8 citation statements)

References 13 publications

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“…The viability and virulence of selected B. brongniartii formulations with various carrier materials were evaluated against H. serrata [338]. Supplements such as calcium chloride, chitin, lactic acid and polyethylene glycol 6000 used with molasses-based liquid media selectively enhanced spore production of three EPF [339,340]. Compatibility of pesticides with three species of EPF was examined and strategies for the integration of the two tools were suggested [341].…”

Section: Othersmentioning

confidence: 99%

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A 100 years of biological control of sugarcane pests in India: review and perspective.

Srikanth¹

2016

CABI Reviews

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Insect pests constitute a major biotic stress in sugarcane in India as they attack the crop from the time of planting until almost harvest, inflicting yield and sugar losses. Biological control has always received a prominent position among the pest management tools, facilitated by the unique semi-perennial crop habitat and low pesticide usage. Biocontrol research of the early 1930s and 40s was characterized by surveys focusing on identification and studies on the basic biology of natural enemies. Conservation and redistribution , and introduction and colonization of predominant parasitoids was practiced very early, and even in the recent past, with remarkable success. Mass multiplication and field evaluation that began in the early decades continue today, as is demonstrated by the use of the most exploited parasitoid Trichogramma chilonis. Several parasitoids and predators of borers and sucking pests were investigated systematically when the need arose. Among entomopathogens, granulosis viruses and fungi received considerable attention; a simple formulation of Beauveria brongniartii reached commercial production for the control of the white grub Holotrichia serrata. In recent years, isolates of Bacillus thuringiensis from sugarcane soil have been examined and a scarabaeid-specific cry gene has been identified. Preliminary studies of kairomonal principles from borers as attractants to the larval parasitoid Cotesia flavipes have been carried out. Organizational support to the cause of biological control includes coordinated research efforts from government agencies, production of biocontrol agents by commercial insectaries and promotion of technologies by the sugar industry. In this review, we chronicle the major research findings over the past eight decades, portray an overview of their significance and project the prospects and priorities for biological control research and promotion in the country.

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Section: Othersmentioning

confidence: 99%

“…Following preliminary studies on B. brongniartii against white grubs [209][210][211], mass culture and formulation techniques were developed for the fungus using sugar industry by-products [336][337][338]. Subsequently, the mass culture methods were further improved by supplementing the culture media with additives [339,340].…”

Section: Research Progressmentioning

confidence: 99%

A 100 years of biological control of sugarcane pests in India: review and perspective.

Srikanth¹

2016

CABI Reviews

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“…연구되었으며 (Srikanth and Santhalakshmi, 2012; 곤충병원성곰팡이의 천연제오라이트 세라믹볼 고체배지 에서 대량 생산된 포자의 분리 포자 대량생산을 위해 천연제오라이트 세라믹볼 2 kg을 이용하여 최적화된 조건인 미강추출물 3 mL, 전분 33 g, 포 자현탁액 7 mL, NaCl 5%를 세라믹볼 용량비율에 맞춰 증 가시킨 후 pH 3으로 조정하여 온도 28℃, 습도 40%로 배양 후 (Fig. 6) …”

Section: 종래의 경우 곰팡이의 포자 생산방법으로 고체 배양이 많이unclassified

Culture Method of Spore for Entomopathogenic Fungus Using Natural Zeolite Ceramic Ball

Lee¹,

Kim²,

Joo³

et al. 2016

Korean Journal of Environmental Agriculture

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“…Media components may influence the spore yield, spore virulence, the activity of virulence-related enzymes such as Pr1, desiccation tolerance, and other relevant attributes of spores [ 17 ]. The addition of glucose, lecithin, collagen, or calcium chloride to liquid culture medium has been found to affect the production of entomopathogenic fungal spores [ 18 , 19 , 20 ]. For example, additives such as lecithin, collagen, or lactic acid into culture broth increased the production of Metarhizium anisopliae blastospores, but the virulence of the spores was weaker than that of spores produced on media without additive [ 19 ].…”

mentioning

confidence: 99%

“…Addition of yeast extract and peptone into liquid media improved both the production of Beauveria brongniartii spores and their virulence against T. vaporariorum [ 22 ]. The yield of B. brongniartii spores was found to vary depending on the combination and doses of additives used for their production, with the additives investigated including calcium chloride, chitin, polyethylene glycol, lactic acid, lecithin, molasses, and nutrient media [ 20 ]. Conidia of B. bassiana and M. anisopliae produced using different medium compositions or osmotic stress medium showed differences in their germination rate, Pr1 activity, and virulence [ 23 , 24 ].…”

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confidence: 99%

Influence of Additives on the Yield and Pathogenicity of Conidia Produced by Solid State Cultivation of an Isaria javanica Isolate

et al. 2014

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Recently, the Q biotype of tobacco whitefly has been recognized as the most hazardous strain of Bemisia tabaci worldwide, because of its increased resistance to some insecticide groups. As an alternative control agent, we selected an Isaria javanica isolate as a candidate for the development of a mycopesticide against the Q biotype of sweet potato whitefly. To select optimal mass production media for solid-state fermentation, we compared the production yield and virulence of conidia between 2 substrates (barley and brown rice), and we also compared the effects of various additives on conidia production and virulence. Barley was a better substrate for conidia production, producing 3.43 × 1010 conidia/g, compared with 3.05 × 1010 conidia/g for brown rice. The addition of 2% CaCO3 + 2% CaSO4 to barley significantly increased conidia production. Addition of yeast extract, casein, or gluten also improved conidia production on barley. Gluten addition (3% and 1.32%) to brown rice improved conidia production by 14 and 6 times, respectively, relative to brown rice without additives. Conidia cultivated on barley produced a mortality rate of 62% in the sweet potato whitefly after 4-day treatment, compared with 53% for conidia cultivated on brown rice. The amendment of solid substrate cultivation with additives changed the virulence of the conidia produced; the median lethal time (LT50) was shorter for conidia produced on barley and brown rice with added yeast extract (1.32% and 3%, respectively), KNO3 (0.6% and 1%), or gluten (1.32% and 3%) compared with conidia produced on substrates without additives.

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Effect of Media Additives on the Production of Beauveria brongniartii, an Entomopathogenic Fungus of Holotrichia serrata

Cited by 9 publications

References 13 publications

A 100 years of biological control of sugarcane pests in India: review and perspective.

A 100 years of biological control of sugarcane pests in India: review and perspective.

Culture Method of Spore for Entomopathogenic Fungus Using Natural Zeolite Ceramic Ball

Influence of Additives on the Yield and Pathogenicity of Conidia Produced by Solid State Cultivation of an Isaria javanica Isolate

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