Effect of meiotic status, cumulus cells and cytoskeleton stabilizer on the developmental competence of ovine oocytes following vitrification

Mo, Xianhong; Fu, Xiangwei; Yuan, Dao; Wu, Guoquan; Jia, Baoyu; Cheng, Keren; Du, Min; Zhou, Yanhua; Yue, Mingxing; Hou, Yunpeng; Li, J.J.; Zhu, Shengming

doi:10.1016/j.smallrumres.2014.01.001

Cited by 12 publications

(9 citation statements)

References 39 publications

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“…Mo et al (2014) also reported similar results for survival, cleavage and blastocyst formation rates for ovine matured oocytes with or without cumulus cells after warming. However, Shirazi et al (2012) reported that when vitrification solution contained 20% fetal bovine serum, higher rate of cleavage was observed for sheep COC (53%) than for denuded oocytes (41%).…”

Section: Cumulus Cells: Coc Vs Denuded Oocytessupporting

confidence: 57%

“…However, when oocytes were pre-treated with taxol for 30 min before vitrification, the survival rate after vitrification and the developmental competence were improved. This study showed that the cryosurvival and cleavage rates of taxol treated oocytes were higher than for those treated with and control oocytes (Mo et al, 2014).…”

Section: Stabilizers Of Cytoskeletonmentioning

confidence: 56%

“…Shirazi et al (2012) found that despite the lack of difference in survival rate of ovine oocytes vitrified at MII or GV, matured oocytes showed a higher resistance to vitrification with cleavage rate of 53%, higher than the rate obtained for immature oocytes (37%). Mo et al (2014) also demonstrated that the meiotic status determines the ability of sheep oocytes to survive vitrification while MII oocytes showed the highest survival rates and developmental competence after vitrification. Purohit et al (2012) reported that immature cumulus compact goat oocytes survived better to vitrification than immature ones.…”

Section: Meiotic Stage: Matured Vs Immaturementioning

confidence: 89%

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Factors that affect oocyte vitrification in small ruminants

Chaves

Souza-Fabjan

Mermillod

et al. 2014

RBCV

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At the end of its growth, the mammalian oocyte, include in preovulatory follicle, is the largest cell of the organism, with about 120 µm in diameter. The size of oocyte, together with the specific arrangement of its organels and cytoskeleton, makes a real challenge for cryopreservation techniques. For such large cell, methods that do not require equilibrium to cryopreservation, such as vitrification, are more promising. It is important to highlight that the cryopreservation of oocytes is more difficult than zygotes or later stage embryos and this technique is a challenging task because of oocyte sensitive nature to chilling and toxic effects of cryoprotectants. However, the development of a reliable method for oocyte cryopreservation would be an important advance in the field of reproductive biology for the preservation of genetic resources. The vitrification of mammalian oocytes is influenced by many variables, such as different cryoprotectants, vitrification techniques, presence or absence of cumulus cells, the oocyte structure, metabolism (such as level of lipid storage) and meiotic stage. Thus, all these factors should be considered to optimize the techniques and adapt them to oocytes from each species. Therefore, the present review aims to describe the main factors that affect oocyte vitrification in sheep and goats, reporting the main findings in both species, as well as perspectives of future improvements.Keywords: COC, cryopreservation, gamete, goat, sheep. ResumoNo fim do seu crescimento, o oócito mamífero é a maior célula do organismo, com cerca de 120 µm de diâmetro. O tamanho do oócito em conjunto com a disposição específica das organelas e do citoesqueleto faz com que ele represente um verdadeiro desafio para as técnicas de criopreservação. Para grandes células, métodos que não exigem equilíbrio para criopreservação, como a vitrificação, são mais promissores. É importante ressaltar que a criopreservação de oócitos é mais difícil que de zigotos ou embriões em estádios mais tardios e esta técnica representa um desafio devido à natureza sensível do oócito ao resfriamento e aos efeitos tóxicos de crioprotetores. Entretanto, o desenvolvimento de uma técnica confiável para a criopreservação oocitária representaria um avanço importante para a preservação de recursos genéticos. A vitrificação de oócitos em mamíferos é influenciada por muitas variáveis , tais como diferentes crioprotetores, técnicas de vitrificação, presença ou ausência de células do cumulus, estrutura do oócito, metabolismo (como o nível de armazenamento de lipídios) e estágio meiótico. Assim, todos esses fatores devem ser considerados quando o objetivo é otimizar as técnicas e adaptá-las para oócitos de cada espécie. Assim, a presente revisão tem como objetivo descrever os principais fatores que afetam a vitrificação de oócitos em ovinos e caprinos, relatando os principais resultados em ambas as espécies, bem como as perspectivas de melhorias futuras.

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Section: Cumulus Cells: Coc Vs Denuded Oocytessupporting

confidence: 57%

Section: Stabilizers Of Cytoskeletonmentioning

confidence: 56%

Section: Meiotic Stage: Matured Vs Immaturementioning

confidence: 89%

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Factors that affect oocyte vitrification in small ruminants

Chaves

Souza-Fabjan

Mermillod

et al. 2014

RBCV

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“…Shirazi et al (2012) found that despite the lack of difference in survival rate of ovine oocytes vitrified at MII or GV, matured oocytes showed a higher resistance to vitrification with cleavage rate of 53%, higher than the rate obtained for immature oocytes (37%). Mo et al (2014) also demonstrated that the meiotic status determines the ability of sheep oocytes to survive vitrification while MII oocytes showed the highest survival rates and developmental competence after vitrification. Quan et al (2014) compared the vitrification of immature with MII goat oocytes and described that the rate of vitrified/thawed MII oocytes with normal morphology and cleavage rate after parthenogenetic activation were significantly higher than 431vitrified/thawed GV oocytes.…”

Section: Effect Of Developmental Stagementioning

confidence: 89%

In Vitro Fertilization of Vitrified Immature or Mature Oocytes as Affected by Mechanical or Natural Denudation

Gabr

Nowier

2016

Egyptian Journal of Nutrition and Feeds

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he current study aimed to evaluate the role of cumulus-corona radiate complex of immature or in vitro matured bovine oocytes on their vitrification, in vitro fertilization and embryo development. Bovine ovaries were collected from an abattoir and follicular oocytes (3-8 mm in diameter) were aspirated. Oocytes were examined and classified into cumulus oocytes-complexes (COCs), natural denuded oocytes (NDOs) and mechanically denuded oocytes (MDOs). Natural COCs were mechanically denuded by repeated pipetting in phosphate buffer solution (PBS). All types of oocytes were cryopreserved by vitrification as immature or in vitro matured for 24 h prior to vitrification by open-pulled straw cryodevice. After at least 2 weeks of vitrification, all types of vitrified oocytes were evaluated for survival and viability (Normal and abnormal oocytes). Morphologically normal immature oocytes were matured and fertilized in vitro, while morphologically normal mature oocytes were directly fertilized in vitro for determination of cleavage rate (CR). After co-culture of cleaved oocytes for 7 days, rate of morula (MPR) and/or blastocysts (BPR) production was recorded. Results show that survival (SR) and normality (NR) rates were the highest for CCOs (84.47 and 81.73%), followed by MDOs (73.28 and 62.0%), and the lowest for NDOs (60.71 and 54.39%), respectively (P<0.05). SR was higher (P<0.05) for mature than immature oocytes (78.03 vs. 67.60%), while NR was nearly similar for both. CR, MPR and BPR were higher (P<0.05) for CCOs (45.28, 18.05 and 15.28%) than those of MDOs (29.72, 6.06 and 3.03%) and NDOs (25.24, 7.69 and 7.69%), respectively. CR was higher (P<0.05) in mature than in immature oocytes (41.93 vs. 29.36%), while MPR and BPR were nearly similar in both. Based on the obtained results, cumulus cell surrounding cumulus oocytes-complexes play a vital role for survival of bovine oocytes during vitrification and successful in vitro maturation and fertilization as well as embryonic development to morula and blastocyst stages.

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“…Therefore, efforts should be made to further improve the efficiency of mammalian oocyte cryopreservation, especially in small ruminant species including sheep and goats. Given these facts, several investigations have used different strategies to enhance the developmental ability of vitrified‐warmed ovine oocytes, such as utilization of different cryo‐devices (Fernández‐Reyez et al, ; Quan et al, ; Succu et al, ) and various combinations of cryoprotectants (Kumar, Gautam, & Gahlawat, ), pretreatment of oocyte with cytoskeletal stabilizers like cytochalasin B (Shirazi et al, ; Silvestre, Yaniz, Salvador, Santolaria, & Lopez‐Gatius, ; Zhang, Nedambale, Yang, & Li, ), introduction of trehalose into cytoplasm (Berlinguer et al, ), vitrification at various developmental stages (Mo et al, ) and addition of nonpenetrating CPAs (Chen et al, ; Eroglu, ; Eroglu, Bailey, Toner, & Toth, ; Wright, Eroglu, Toner, & Toth, ). Nonpenetrating CPAs such as sucrose and trehalose are known to reduce possible toxic effects of penetrating CPAs and avoid cell swelling during vitrification and warming procedure.…”

Section: Introductionmentioning

confidence: 99%

Developmental competence of in vitro matured ovine oocytes vitrified in solutions with different concentrations of trehalose

Sanaei

Movaghar

Valojerdi

et al. 2018

Reprod Domestic Animals

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This study aimed to determine the optimum concentration of trehalose in solutions used for vitrification of in vitro matured (IVM) ovine oocytes. IVM oocytes were randomly divided into four experimental (vitrified) and one control (fresh) groups. Experimental groups were treated with different concentrations (0.0, 0.25, 0.5 and 1.0 M) of trehalose. After warming, some viable oocytes were exposed to 0.25% pronase to test zona pellucida hardening, whereas the others were fertilized and cultured in vitro for 8 days to evaluate their developmental competence. Blastocysts quality was assessed by differential staining and TUNEL test. Survival and developmental rates of oocytes vitrified in the presence of 0.5 M trehalose were significantly higher than those of the other vitrified groups. Furthermore, there was a significant difference between fresh and vitrified groups in total blastocyst rate. Analysis of blastocysts quality also revealed a significant difference between the group treated with 0.5 M trehalose and other groups in terms of apoptotic index. Furthermore,zona pellucida digestion time period was longer in trehalose-free (0.0 M) group compared to other groups. In conclusion, we found that IVM ovine oocytes vitrified in solutions containing 0.5 M trehalose are fertilization-competent and are able to produce good-quality blastocysts with an apoptotic index comparable to that of the fresh oocytes. Therefore, 0.5 M may be considered the optimum concentration of trehalose to be used in solutions prepared for vitrification of oocytes.

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Effect of meiotic status, cumulus cells and cytoskeleton stabilizer on the developmental competence of ovine oocytes following vitrification

Cited by 12 publications

References 39 publications

Factors that affect oocyte vitrification in small ruminants

Factors that affect oocyte vitrification in small ruminants

In Vitro Fertilization of Vitrified Immature or Mature Oocytes as Affected by Mechanical or Natural Denudation

Developmental competence of in vitro matured ovine oocytes vitrified in solutions with different concentrations of trehalose

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