Abstract:The objectives of this study are to find out the effect of melatonin treatments (43µM & 430µM) on mRNA and protein expression of casein, and melatonin receptors in buffalo mammary epithelial cells (BuMEC) using reverse transcriptional polymerase chain reaction (qRT-PCR) and Western blotting. Furthermore, by using specific inhibitors against mTOR (Rapamycin) and Jak2-Stat5 pathway (AG490), effect of melatonin treatment on translational level of casein was measured. The results showed that melatonin treatment (4… Show more
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