Effect of melatonin on epididymal sperm quality after testicular ischemia/reperfusion in rats

Kurçer, Zehra; Hekimoğlu, Aşkın; Aral, Faruk; Baba, Füsun; Şahna, Engin

doi:10.1016/j.fertnstert.2009.01.146

Cited by 41 publications

(36 citation statements)

References 31 publications

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“…Our findings are in accordance with previously described detrimental effects of I/R on sperm characteristics in the treated rats [17,20,32]. In our study, ghrelin injection did not prevent I/Rinduced reduction in membrane integrity, but it clearly caused statistically significant increases in sperm motility, movement and concentration in the testes of rats compare to the I/R group ( Table 2).…”

Section: Discussionsupporting

confidence: 93%

“…In our study, ghrelin injection did not prevent I/Rinduced reduction in membrane integrity, but it clearly caused statistically significant increases in sperm motility, movement and concentration in the testes of rats compare to the I/R group ( Table 2). The decrease in sperm quality may be due to IR-induced oxidative stress in testicular tissue [17]. The relationship between oxidant/antioxidant status of testicular tissue and sperm quality has been well-documented [4,31].…”

Section: Discussionmentioning

confidence: 99%

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The effect of ghrelin pretreatment on epididymal sperm quality and tissue antioxidant enzyme activities after testicular ischemia/reperfusion in rats

et al. 2011

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Ghrelin, the endogenous ligand for growth hormone secretagogue receptor, has been reported to prevent ischemia/reperfusion (I/R) injury in various tissues by its antioxidant activity. Therefore, this study was aimed to investigate the effect of ghrelin on sperm quality and antioxidant enzyme activity in a rat testicular ischemia/reperfusion injury model. Forty-two male Wistar rats were divided into groups control, I/R, and I/R plus ghrelin. The right testes were rotated 720° for 1 h and were allowed to reperfuse for 4 h and 30 days thereafter. Ghrelin (40 nmol/kg IP) or vehicle (physiological saline) was administrated 15 min before reperfusion. After 4 h of reperfusion, a right orchiectomy was performed to measure the biochemical parameters. In addition, the sperm was collected from the epididymis after 30 days of reperfusion, and sperm characteristics were examined. The malondialdehyde levels of the testis tissues were significantly increased, but a statistically significant decrease was found in the superoxide dismutase, glutathione peroxidase, and catalase activities in the I/R group as compared with the control, indicating I/R injury. The sperm evaluation showed a significant reduction in all characteristics resulted from I/R compared with the control. In the ghrelin-treated group, the malondialdehyde values were significantly lowered, and only enzyme activity of glutathione peroxidase showed significant increases compared with the I/R group. Ghrelin significantly enhanced sperm motility, movement, and concentration but did not prevent I/R-induced reduction in membrane integrity in the testes of rats compared to the I/R group. Our results suggest that ghrelin treatment has a protective role on IR-induced testicular injury, and this effect may be due to its antioxidant properties.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

The effect of ghrelin pretreatment on epididymal sperm quality and tissue antioxidant enzyme activities after testicular ischemia/reperfusion in rats

et al. 2011

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“…Some authors have reported that melatonin treatment induced a marked reduction in sperm quality and did not prevent the reduction in sperm concentration under an ischemia/reperfusion condition (Oosthuizen et al 1986;Kurcer et al, 2010), which concurs with the results of the present work. Luboshitzky et al (2002) reported that melatonin administration (3mg) is associated with decreased semen quality in healthy men, probably through the inhibition of aromatase at the testicular level.…”

Section: Discussionsupporting

confidence: 92%

Melatonin Protects the Heart, Lungs and Kidneys from Oxidative Stress under Intermittent Hypobaric Hypoxia in Rats

2012

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Melatonin (N-acetyl-5-methoxytryptamine) is the main secretory product of the pineal gland in all mammals including humans, but it is also produced in other organs. It has been previously demonstrated to be a powerful organ-protective substance under oxidative stress conditions. The aim of this study was to evaluate the protective effect of melatonin in several organs such as heart, lung, kidney, and of the reproductive system, such as testis and epididymis in animals exposed to intermittent hypobaric hypoxia and therefore exposed to oxidative stress and analyzed by lipid peroxidation. Ten-week-old male Wistar rats were divided into 6 groups for 96 hours during 32 days under: 1) Normobaric conditions, 2) plus physiologic solution, 3) plus melatonin, 4) intermittent hypobaric hypoxia, 5 plus physiologic solution and 6) plus melatonin. The animals were injected with melatonin (10 mg/kg body weight) at an interval of 96 hours during 32 days. Results indicated that melatonin decreased lipid peroxidation in heart, kidneys and lung under intermittent hypobaric hypoxia conditions. However, it did not exhibit any protective effect in liver, testis, epididymis and sperm count.

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“…In fact, melatonin decreased the rate of abnormal sperm caused by ischemia-reperfusion in rats (Kurcer et al, 2010).…”

Section: Discussionmentioning

confidence: 88%

Melatonin Protective Role in Mouse Cauda Epipidymal Spermatozoa Damage Induced by Sodium Arsenite

et al. 2013

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SUMMARY:We evaluated the sperm parameters such as cauda epididymis weight, sperm count, sperm morphology and sperm DNA stability of adult CF-1 male mice treated daily (oral exposure) with the toxic sodium arsenite (As, 7.0 mg/kg/body weight); Melatonin (Me, 10.0 mg/kg/bw), Me (10.0 mg/kg/bw) plus As (7.0 mg/kg/bw) and Negative Control (NaCl 0.9%) to assess acute (8.3 days), chronic (33.2 days) and recovery of testicular damage (66.4 days). Arsenic decreases the number of sperm from chronic treatment (33.2 days) and this effect continued until 66.4 days of treatment. The toxic effect of As also altered the morphology of spermatozoa in all treatment periods when compared to the negative control group. However, Metalonin induced protective effects in periods of 33.2 and 66.4 days of treatment. Additionally, the stability of DNA was significantly affected by arsenic in all periods, but the chronic treatment (33.2 days) in the AsMe revealed increased stability compared to the group treated with arsenic only. Melatonin partially protects sperm toxicity caused by Arsenic, especially during periods of 33.2 and 66.4 days. 1252In this work, we aim to determine the effects of acute, chronic and recovery of testicular exposure to sodium arsenite in epididymal sperm and assess the use of melatonin as a protective agent and antioxidant. MATERIAL AND METHODThree-month-old male mice (Mus musculus) strain CF-1, healthy and sexually mature, were obtained from the Faculty of Medicine, University of Chile. The animals were maintained in a room under controlled conditions of temperature (22±2°C), and a 12 h light/dark cycle, with ad libitum access to commercial pellets and tap water.Reproductive changes were studied as acute (8.3 days), chronic (33.2 days) and recovery of testicular damage (66.4 days) in 88 adult mice exposed every day to oral doses of Sodium Arsenite (As; 7.0 mg/kg/bw, Sigma Chemical Co., St. Louis, MO); Melatonin (Me; 10.0 mg/kg/bw, Arama Laboratorios SA, Santiago, Chile) (Gutelkin et al., 2001); Me (10.0 mg/kg/bw) plus As (7.0 mg/kg/bw), and Negative Control (NaCl 0.9%) delivered orally in a volume not greater than 0.05 mL.Before the beginning and after the end of treatment, all animals were weighted. Euthanasia was performed by cervical dislocation after anesthesia with ketamine (Laboratory Biosano SA, Santiago, Chile) according to the protocol of animal handling of the Bioethics Committee of the Faculty of Medicine, University of Chile. Sperm Parameters.Extraction, weighing and sperm count in epididymalcauda. After obtaining the cauda epididymis they were weighed. Then sperm suspension was obtained using the protocol by Fornés & Bustos-Obregón (1994). Sperm were counted at 200X in a microscope, equipped to use Makler® chamber, adding a drop of sperm suspension by capillary and counting in duplicate the sperm present in the 100 squares of the camera, expressing this result as a million sperm/mg epididymis. Sperm morphology analysis.From the sperm suspension obtained above it was obtained 200 µL of sample. Addit...

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Effect of melatonin on epididymal sperm quality after testicular ischemia/reperfusion in rats

Cited by 41 publications

References 31 publications

The effect of ghrelin pretreatment on epididymal sperm quality and tissue antioxidant enzyme activities after testicular ischemia/reperfusion in rats

The effect of ghrelin pretreatment on epididymal sperm quality and tissue antioxidant enzyme activities after testicular ischemia/reperfusion in rats

Melatonin Protects the Heart, Lungs and Kidneys from Oxidative Stress under Intermittent Hypobaric Hypoxia in Rats

Melatonin Protective Role in Mouse Cauda Epipidymal Spermatozoa Damage Induced by Sodium Arsenite

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