Effect of Melatonin on the Spermatogenesis of Rana Hexadactyla(Lesson).

Kasinathan, Sivakanthan; Gregalatchoumi, S

doi:10.1507/endocrj1954.35.255

Cited by 9 publications

(10 citation statements)

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“…The results of the present study revealed that a 50 mg/kg exposure to cadmium induces significant apoptosis of spermatogonia and spermatocytes in the testes of adult Iberian ribbed newts, indicating that germ cells in these differentiation stages are highly sensitive to cadmium. The histopathologic findings of the present study are similar to those reported for cadmium-exposed frogs, such as B. melanostictus and R. hexadactyla Lesson , and fish, such as O. latipes 24 , 25 , 26 . To our knowledge, this is the first report of the histomorphologic characteristics of cadmium-induced testicular dysfunction in newts.…”

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confidence: 88%

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Effects of cadmium exposure on Iberian ribbed newt (<i>Pleurodeles waltl</i>) testes

et al. 2017

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To characterize the histomorphologic effects of cadmium on adult newt testes, male Iberian ribbed newts (6 months post-hatching) were intraperitoneally exposed to a single dose of 50 mg/kg of cadmium, with histologic analysis of the testes at 24, 48, 72, and 96 h. Beginning 24 h after cadmium exposure, apoptosis of spermatogonia and spermatocytes was observed, and congestion was observed in the interstitial vessels of the testes. Throughout the experimental period, the rates of pyknotic cells and TUNEL and cleaved caspase-3 positivity were significantly higher in the spermatogonia and spermatocytes of cadmium-treated newts compared with control newts. There were no significant differences between cadmium-treated and control newts in phospho-histone H3 positivity in the spermatogonia and spermatocytes. These results suggest that spermatogonia and spermatocytes in adult Iberian ribbed newts are highly sensitive to cadmium. This is the first report of the histomorphologic characteristics of cadmium-induced testicular dysfunction in newts.

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supporting

confidence: 88%

“…injection of 50 mg/kg of cadmium induced apoptosis of the spermatogonia and spermatocytes in Iberian ribbed newts. The observed histopathologic findings in the testes of newts exposed to cadmium were similar to those reported for testes of several frogs, such as Bufo melanostictus and Rana hexadactyla Lesson 24 , 25 , and fish, such as Oryzias latipes 26 .…”

supporting

confidence: 84%

Effects of cadmium exposure on Iberian ribbed newt (<i>Pleurodeles waltl</i>) testes

et al. 2017

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“…Another study carried out by Kasinathan et al [29] showed that Cd significantly reduces primary and secondary spermatocyte in seminiferous tubules.…”

Section: Groups Mean Johnsen Scorementioning

confidence: 95%

Cadmium Toxicity in Spermatogenesis and Protective Effects of l-Carnitine in Adult Male Rats

Yari

Asadi

Bahadoran

et al. 2009

Biol Trace Elem Res

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In this study, the effects of cadmium toxicity and the protective effects of L-carnitine on spermatogenesis in Sprague-Dawley rat were evaluated. Animals were subdivided into five groups. Cadmium chloride (1-mg/kg body weight) was injected intraperitoneally during 16 days at intervals of 48 h between subsequent treatments. L-carnitine (500 mg/kg b.w., IP) was pretreated in both of control and cadmium-injected rats. Animals were killed on day 17 after the first treatment. The left cauda epididymis was removed and immediately immersed into Hank's balanced salt solution for evaluation of sperm count and viability. Following contamination with cadmium, a decrease in the number and viability of cauda epididymis sperm, the number of cell proliferation, and Johnsen Scores in the seminiferous tubules was observed. Consequently, L-carnitine treatment caused an increase in the number and viability of cauda epididymis sperm, the number of cell proliferation, and Johnsen Scores in the cadmium-induced group.

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“…Exposure to cadmium can induce germ cell apoptosis, which may account for the current decline in male fertility ( Akinloye et al, 2006 ). Another study, carried out by Kasinathan et al . (1987) showed that cadmium significantly decreased primary and secondary spermatocytes in the seminiferous tubules.…”

Section: Discussionmentioning

confidence: 99%

Effects of the methanol root extract of Carpolobia lutea on sperm indices, acrosome reaction, and sperm DNA integrity in cadmiuminduced reproductive toxicity in male Wistar rats

Akinola

Oyeyemi

Daramola

et al. 2020

JBRA Assisted Reproduction

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Objective: Oxidative stress is a mechanism of cadmium-induced reproductive dysfunction. Carpolobia lutea is a free radical scavenger. Our study investigated the potential protective effects of Carpolobia lutea root methanol extract against cadmium-induced reproductive toxicity. Methods: We obtained the Carpolobia lutea root in Akure, and it was authenticated at the Forestry Research Institute of Nigeria (FRIN) herbarium, Ibadan, Nigeria, with FHI number 109784. We used Soxhlet extraction to obtain its methanol extract. We used thirty male Wistar rats (150-170g) in this study, (n=5 per group), and treated them as follows: Control (1 ml/kg normal saline), Cd (2 mg/kg), Cd+MCL (2 mg/kg+100 mg/kg), Cd+MCL (2 mg/kg+200 mg/kg), MCL (100 mg/kg), MCL (200 mg/kg). We administered Carpolobia lutea orally for 8 weeks. We administered a single dose of 2 mg/kg of cadmium intraperitoneally. We assessed the sperm profile using a computer-aided sperm analyzer. Under microscopy, we determined the sperm acrosome reaction and the DNA damage. We measured the seminal fructose level using spectrophotometry, and the data were analyzed using ANOVA at p <0.05. Results: Cd+MCL (2mg/kg+200 mg/kg) significantly increased sperm count (339.0±25.0 vs. 29.0±4.5 million/mL), motility (80.0±0.2 vs. 55.0±4.9%), viability (68.7±2.7 vs. 31.3±2.9%) and decreased abnormal sperm (28.3±1.7 vs. 43.3±2.5%), relative to the cadmium group. Cd+MCL (2mg/kg+200 mg/kg) significantly increased acrosome reaction (68.0±7.5 vs. 15.2±2.4%) and seminal fructose level (0.49±0.06 vs. 0.28±0.06 mmol/L) relative to the cadmium group. Cd+MCL (2mg/kg+200 mg/kg) significantly decreased sperm DNA damage (14.1±1.6 vs. 35.9±5.3%) in relation to the cadmium group. Conclusions: Carpolobia lutea root extract improves the sperm variables of rats exposed to cadmium.

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Effect of Melatonin on the Spermatogenesis of Rana Hexadactyla(Lesson).

Cited by 9 publications

References 1 publication

Effects of cadmium exposure on Iberian ribbed newt (<i>Pleurodeles waltl</i>) testes

Effects of cadmium exposure on Iberian ribbed newt (<i>Pleurodeles waltl</i>) testes

Cadmium Toxicity in Spermatogenesis and Protective Effects of l-Carnitine in Adult Male Rats

Effects of the methanol root extract of Carpolobia lutea on sperm indices, acrosome reaction, and sperm DNA integrity in cadmiuminduced reproductive toxicity in male Wistar rats

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