Effect of Melatonin on the Extracellular-Regulated Kinase Signal Pathway Activation and Human Osteoblastic Cell Line hFOB 1.19 Proliferation

Xiong, Xiaolu; Zhu, Yue; Ge, Rui; Liu, Lifeng; Yuan, Wei

doi:10.3390/ijms160510337

Cited by 21 publications

(11 citation statements)

References 56 publications

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“…It mainly depends on the following mechanisms based on the G-protein-coupled receptor (MT2): enhancing osteoblast activity, inhibiting osteoclast differentiation and scavenging oxygen free radicals. We also found that melatonin promotes osteoblast proliferation in a concentration-dependent manner (Liu et al, 2011;Liu et al, 2012;Xiong et al, 2015;Tao and Zhu, 2018). Notably, melatonin significantly induces osteoblast differentiation and matrix mineralization, but the underlying mechanism remains unclear (Roth et al, 1999;Park et al, 2011;Maria et al, 2018).…”

Section: Introductionsupporting

confidence: 56%

Protective Role of Melatonin Against Postmenopausal Bone Loss via Enhancement of Citrate Secretion From Osteoblasts

Lin

Wen

et al. 2020

Front. Pharmacol.

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A negative correlation exists between the severity of osteoporosis and citrate levels in bone. Our previous research found that melatonin can significantly improve bone mass in mice with osteoporosis, but the underlying mechanism involving citrate remains unknown. Herein, we demonstrated that melatonin increased bone volume and citrate levels in ovariectomized osteoporosis mice. Melatonin increased citrate and mineralized nodules in osteoblasts induced from primary mouse bone marrow mesenchymal stem cells in vitro. ZIP-1 knockdown and overexpression confirmed that melatonin specifically upregulated ZIP-1 to rescue citrate levels and bone mass. In general, we verified that melatonin can improve bone mass by enhancing matrix mineralization, which is highly related to increased citrate secretion from osteoblasts, and that ZIP-1 is the target of melatonin. These findings reveal another role of melatonin in regulating bone remodeling and provide a research base for its possible application in the treatment of clinical osteoporosis in the future.

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Section: Introductionsupporting

confidence: 56%

Protective Role of Melatonin Against Postmenopausal Bone Loss via Enhancement of Citrate Secretion From Osteoblasts

Lin

Wen

et al. 2020

Front. Pharmacol.

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“…However, some studies have noted that melatonin has a negative effect on bone [32]. Based on the existing literature, we chose four melatonin concentrations that respectively have had positive and negative effects in vivo and in vitro:100 mg/kg and 50 mg/kg melatonin for in vivo tests and 10μM and 1 mM melatonin for in vitro tests [7, 8, 33, 34]. Our experiments demonstrated for the first time that melatonin suppresses osteoporosis in diabetes mellitus.…”

Section: Discussionmentioning

confidence: 99%

Melatonin suppresses autophagy in type 2 diabetic osteoporosis

et al. 2016

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Type 2 diabetes mellitus is often complicated by osteoporosis, a process which may involve osteoblast autophagy. As melatonin suppresses autophagy under certain conditions, we its investigated the effects on bone autophagy during diabetes. We first assessed different body parameters in a diabetic rat model treated with various concentrations of melatonin. Dynamic biomechanicalmeasurements, bone organization hard slice dyeing and micro-CT were used to observe the rat bone microstructure, and immunohistochemistry was used to determine levels of autophagy biomarkers. We also performed in vitro experiments on human fetal osteoblastic (hFOB1.19) cells cultured with high glucose, different concentrations of melatonin, and ERK pathway inhibitors. And we used Western blotting and immunofluorescence to measure the extent of osteogenesis and autophagy. We found that melatonin improved the bone microstructure in our rat diabetes model and reduced the level of autophagy(50 mg/kg was better than 100 mg/kg). Melatonin also enhanced osteogenesis and suppressed autophagy in osteoblasts cultured at high glucose levels (10 μM was better than 1 mM). This suggests melatonin may reduce the level of autophagy in osteoblasts and delay diabetes-induced osteoporosis by inhibiting the ERK signaling pathway.

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“…Melatonin inhibits fatty acid-induced triglyceride accumulation in ROS17/2.8 cells, which may promote osteoblast differentiation and inhibit bone loss. 80 Moreover, melatonin enhances osteoblastic differentiation in vitro [81][82][83][84][85][86][87][88] and promotes mouse cortical bone formation in vivo. 81…”

Section: Melatonin Promotes Osteogenesis In Osteoblastsmentioning

confidence: 99%

Melatonin: Another avenue for treating osteoporosis?

Tian

Jiang

et al. 2019

Journal of Pineal Research

111

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Melatonin is a signal molecule that modulates the biological circadian rhythms of vertebrates. Melatonin deficiency is thought to be associated with several disorders, including insomnia, cancer, and cardiovascular and neurodegenerative diseases. Accumulating evidence has also indicated that melatonin may be involved in the homeostasis of bone metabolism. Age‐related reductions in melatonin are considered to be critical factors in bone loss and osteoporosis with aging. Thus, serum melatonin levels might serve as a biomarker for the early detection and prevention of osteoporosis. Compared to conventional antiosteoporosis medicines, which primarily inhibit bone loss, melatonin both suppresses bone loss and promotes new bone formation. Mechanistically, by activating melatonin receptor 2 (MT2), melatonin upregulates the gene expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP2), BMP6, osteocalcin, and osteoprotegerin to promote osteogenesis while inhibiting the receptor activator of NF‐kB ligand (RANKL) pathway to suppress osteolysis. In view of the distinct actions of melatonin on bone metabolism, we hypothesize that melatonin may be a novel remedy for the prevention and clinical treatment of osteoporosis.

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Effect of Melatonin on the Extracellular-Regulated Kinase Signal Pathway Activation and Human Osteoblastic Cell Line hFOB 1.19 Proliferation

Cited by 21 publications

References 56 publications

Protective Role of Melatonin Against Postmenopausal Bone Loss via Enhancement of Citrate Secretion From Osteoblasts

Protective Role of Melatonin Against Postmenopausal Bone Loss via Enhancement of Citrate Secretion From Osteoblasts

Melatonin suppresses autophagy in type 2 diabetic osteoporosis

Melatonin: Another avenue for treating osteoporosis?

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