Effect of membrane parameters and filter structure on the efficiency of leukocyte removal by electrospun poly(ethylene-co-vinyl alcohol) membranes

Serological assay for coronavirus 2019 (COV-ID-19) patients including asymptomatic cases can inform on disease progression and prognosis. A detection method taking into account multiplex, high sensitivity, and a wider detection range will help to identify and treat COVID-19. Here we integrated color-size dualencoded beads and rolling circle amplification (RCA) into a bead-based fluorescence immunoassay implemented in a size sorting chip to achieve high-throughput and sensitive detection. We used the assay for quantifying COVID-19 antibodies against spike S1, nucleocapsid, the receptor binding domain antigens. It also detected inflammatory biomarkers including interleukin-6, interleukin-1β, procalcitonin, C-reactive protein whose concentrations range from pg mL À 1 to μg mL À 1 . Use of different size beads integrating with RCA results in a tunable detection range. The assay can be readily modified to simultaneously measure more COVID-19 serological molecules differing by orders of magnitude.Coronavirus 2019 (COVID-19) caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an unprecedented worldwide pandemic. Serological immunoglobulins and pro-inflammatory cytokines induced by SARS-CoV-2 were observed both in symptomatic patients and some asymptomatic cases. [1] Circulating antibodies specific to SARS-COV-2 and inflammatory biomarkers not only provide evidence for diagnosing a SARS-COV-2 infection and predicting protective immunity but

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A Dual‐Encoded Bead‐Based Immunoassay with Tunable Detection Range for COVID‐19 Serum Evaluation

Lin

Zhang

Zou

et al. 2022

Angewandte Chemie

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A Dual‐Encoded Bead‐Based Immunoassay with Tunable Detection Range for COVID‐19 Serum Evaluation

et al. 2022

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Serological assay for coronavirus disease 2019 (COVID‐19) patients including asymptomatic cases can inform disease progression and prognosis. A detection method taking into account multiplex, high sensitivity, and a wider detection range will help to identify and treat COVID‐19. Here we integrated color‐size dual‐encoded beads and rolling circle amplification (RCA) into bead‐based fluorescence immunoassay implemented in a size sorting chip to achieve high‐throughput and sensitive detection. We used the assay for quantifying COVID‐19 antibodies against spike S1, nucleocapsid, the receptor binding domain antigens, and detecting inflammatory biomarkers including interleukin‐6, interleukin‐1β, procalcitonin, C‐reactive protein whose concentrations range from pg mL ‐1 to μg mL ‐1 as different size beads integrating with RCA result in tunable detection range. The assay can be readily modified to simultaneously measure more COVID‐19 serological molecules differing by orders of magnitude.

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Surface zwitterionization of polypropylene nonwoven fabric by dielectric barrier discharge plasma for efficient leukodepletion

Venault

Chen

Tang

et al. 2023

Journal of Membrane Science

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Effect of membrane parameters and filter structure on the efficiency of leukocyte removal by electrospun poly(ethylene-co-vinyl alcohol) membranes

Cited by 5 publications

References 27 publications

A Dual‐Encoded Bead‐Based Immunoassay with Tunable Detection Range for COVID‐19 Serum Evaluation

A Dual‐Encoded Bead‐Based Immunoassay with Tunable Detection Range for COVID‐19 Serum Evaluation

A Dual‐Encoded Bead‐Based Immunoassay with Tunable Detection Range for COVID‐19 Serum Evaluation

Surface zwitterionization of polypropylene nonwoven fabric by dielectric barrier discharge plasma for efficient leukodepletion

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