Effect of menthol cigarette on rats for metabonomics by liquid chromatography-mass spectrometry

Abstract

A liquid chromatography-mass spectrometry (LC-MS) method was developed for the metabonomics study of menthol cigarette effect on rats. Urines from three groups of rats were analyzed, including control rats, rats treated with menthol cigarette and rats treated with normal cigarette, and the data were processed by the method of principal component analysis (PCA). The PCA score plot showed that the metabolic difference between the rats treated with menthol cigarette and the control rats was smaller than that between the rats treated with normal cigarette and the control rats. Based on the PCA score plot, eight important metabolites, for example, kynurenic acid, were found and identified. Their relative concentration changes among the three groups further indicated that menthol cigarette maybe decrease the metabolic effect on rats.

Characterization and identification of the metabolites of Menthae Haplocalycis Herba water extracts in rat plasma, urine, and feces by ultra‐high performance liquid chromatography with linear ion trap‐Orbitrap mass spectrometry

“…It was detected in urine and feces as well. M6 (t R = 9.47 min) gave [M+Na] + ion at m/z 371.16608 (C 16…”

“…However, current research on the chemical composition of M. Haplocalycis mainly focuses on the volatile components , and lacks studies on the systematic characterization of the chemical components in MHWE. To date, few studies have assessed the metabolism of MHWE in vivo . Metabolite analysis of MHWE and the process of metabolism in MHWE are still unclear till now , which restricts the research regarding its pharmacologic activities.…”

“…To date, few studies have assessed the metabolism of MHWE in vivo . Metabolite analysis of MHWE and the process of metabolism in MHWE are still unclear till now , which restricts the research regarding its pharmacologic activities. There is thus a need for the more comprehensive characterization of the metabolism of MHWE.…”

“…By comparing with the literature[8], M12 was tentatively identified as luteolin-7-Oglucoside. M15, with [M+Na] + ion at m/z 355.17154 (calcd for C16 H 28 O 7 Na) were eluted at 17.73 min. Following CID cleavage, m/z 337, 193, 203 and 185 were produced.…”