Effect of Mercaptoethanol on the Wet Spinning of Wheat Gliadin Fiber

Enhancing gluten detection assay development through optimization of gliadin extraction conditions

“…One of the main components of gluten, gliadin, which is used as a target analyte in the majority of gluten detection assays contain a number of intramolecular disulfide bonds within its structures. These disulfide bonds should be cleaved for effective extraction of gliadin from a sample and as a reductant 2-mercaptoethanol could serve this purpose [ 21 ]. 2-mercaptoethanol has formerly and extensively been used as a component of gliadin extraction buffers within a variety of gluten detection assays [ [22] , [23] , [24] ].…”