Effect of mesenchymal stem cells and platelet-derived growth factor on the healing of radiation induced ulcer in rats

Abstract

Radiation-induced skin ulceration is a frequent complication of radiation therapy. This study investigated the effects of rat mesenchymal stem cells (rMSCs) and platelet-derived growth factor (PDGF) on the healing of radiation-induced soft tissue injury. Sprague-Dawley rats (n=17) were irradiated on the right and left buttocks with a single dose of 50 Gy. The right buttocks were administered with phosphatebuffered solution as a control. The left buttocks were administered with either rMSCs (2×10 6 cells), PDGF (8 µg), or PDGF combined with rMSCs. Administration was done at three weeks after irradiation. Wound healing was analyzed by calculating the percentage of residual ulcerated skin area compared to the total irradiated area during the five week healing period after administration. Modified skin scores were also assessed. Finally, skin lesions were histologically evaluated. More than 40% of the irradiated skin area within the irradiated zone underwent ulceration within 16 days postirradiation, with peak ulceration exceeding 50% around three weeks post-irradiation. Administration of rMSCs or PDGF alone did not confer any significant healing effect. The combined rMSCs+PDGF treatment significantly reduced the wound size compared with the nontreated control up to two weeks postinjection. Regarding the histological examination, lesions administered with PDGF (either alone or mixed with rMSCs) resulted in a greater deposition of highly organized collagen fibers throughout the dermis layer, compared with the control. In conclusion, the combined administration of rMSCs and PDGF efficiently enhanced the healing of radiation-induced skin ulceration.

Wound‐healing effect of adipose stem cell‐derived extracellular matrix sheet on full‐thickness skin defect rat model: Histological and immunohistochemical study

“…Adipose tissue, which is a major endocrine and secretory organ, can be relatively easily collected through the liposuction procedure . It is rich in ECM components such as collagen, elastin, and fibronectin.…”

“…Adipose tissue, which is a major endocrine and secretory organ, can be relatively easily collected through the liposuction procedure. [1][2][3][4][5][6] It is rich in ECM components such as collagen, elastin, and fibronectin. Recently, there has been considerable interest in the tissue engineering of scaffolds fabricated from various living tissues.…”

“…In addition to adipocytes, adipose tissue also contains fibroblasts, smooth muscle cells, endothelial cells, immune cells, and adipose‐derived stem cells. Because of the ease of collection and potential to serve as a source of stem cells, stromal vascular fraction cells have been widely used in clinical applications . However, adipose tissue also contains various extracellular matrix (ECM) components, which might be attractive candidates in the manufacturing of wound‐healing materials .…”

“…Because of the ease of collection and potential to serve as a source of stem cells, stromal vascular fraction cells have been widely used in clinical applications. [4][5][6] However, adipose tissue also contains various extracellular matrix (ECM) components, which might be attractive candidates in the manufacturing of wound-healing materials. 7,8 The ECM is a natural ideal biological scaffold material, which helps retain cells together in tissues and protects and supports the cellular environment.…”

Dual growth factor‐immobilized asymmetrically porous membrane for bone‐to‐tendon interface regeneration on rat patellar tendon avulsion model

“…From these observations, we recognized that the asymmetrically porous PCL/F127 membranes can act as a physical barrier to prevent fibrous tissues invasion and as a scaffold to guide tissue regeneration as well as a carrier for prolonged growth factor release for the regeneration of BTI injury. Sustained release of the single PDGF‐BB and BMP‐2 from the membrane showed the intrinsic efficacy of each growth factor (i.e., promotion of tendon cell proliferation and collagen synthesis by PDGF‐BB, and induction of cartilage and bone formation by BMP‐2); however, the single growth factor system may be insufficient to reconstruct the BTI with a multiphasic structure in our system. On the other hand, the Dual GF system may provide an appropriate environment to regenerate the BTI injury by the GTR/scaffold effect of the asymmetrically porous PCL/F127 membrane as well as the synergistic (complementary) effect of continuously released PDGF‐BB and BMP‐2 to create a multiphasic structure like the native structure.…”