Effect of Meta-topolin on in vitro propagation of Pelargonium × hortorum and Pelargonium × hederaefolium cultivars

Wojtania, Agnieszka

doi:10.5586/asbp.2010.013

Cited by 45 publications

(27 citation statements)

References 24 publications

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“…Susceptibility to early senescence greatly depends on plant species. Our previous [2,3] and this study showed significant differences among Pelargonium genotypes in their susceptibility to leaf yellowing. Early senescence is widely influenced by stress conditions [18].…”

Section: Discussionmentioning

confidence: 99%

“…The concentration of cytokinins was determined on the basis of previous experiments [3]. Explants incubated on a medium without growth regulators served as a control.…”

Section: Growth Regulator Treatmentsmentioning

confidence: 99%

“…It was found that mT enhanced the regeneration capacity of recalcitrant cultivars and stimulated cyclic shoot formation. The observations of different geranium genotypes showed that they differed in their susceptibility to leaf yellowing [2,3].…”

Section: Introductionmentioning

confidence: 99%

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Effects of cytokinins on antioxidant enzymes in in vitro grown shoots of Pelargonium hortorum L. H. Bayley

Wojtania¹,

Skrzypek²

2014

Acta Agrobot

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A b s t r a c tThe aim of this study was to determine the influence of meta-topolin (mT) and 6-benzyl-aminopurine (BAP) on the hydrogen peroxide (H 2 O 2 ) level and antioxidant enzymes activities in relation to the shoot formation and senescence process in Pelargonium hortorum cultivars, which differ in their susceptibility to leaf yellowing under in vitro conditions.In an early senescing cultivar 'Grand Prix', the addition of an aromatic cytokinin mT to abscisic acid (ABA)-enriched Murashige and Skoog (MS) basal medium more efficiently inhibited leaf yellowing than BAP. In both genotypes, meta-topolin was also the most effective in shoot formation. It was found that Pelargonium species varying in their susceptibility to senescence differ in H 2 O 2 production and antioxidant enzymes activities. Generally, meta-topolin more effectively enhanced H 2 O 2 production and POD activity than BAP and control medium, but its effect depended on genotype. The highest H 2 O 2 production stimulated by mT was observed on day 5 of subculture in late senescing cv. 'Bergpalais'. In both geranium genotypes, superoxide dismutase (SOD) and catalase (CAT) levels were highest at the beginning of the subculture period, during the initiation of shoot formation. SOD showed the highest activity on day 5 of subculture on the medium without cytokinin and generally being higher in cv. 'Bergpalais' than in cv. 'Grand Prix'. CAT activity was positively regulated by both cytokinins. POD activity was most effectively enhanced by mT, but on different days of subculture -on the 2 nd day of subculture in cv. 'Bergpalais' and on the 22 nd day of subculture in cv. 'Grand Prix'. The enhanced activity of POD in the presence of mT, 4-fold higher than on control medium, at the end of subculture in P. hortorum 'Grand Prix' coincided with the inhibition of leaf senescence.

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Section: Discussionmentioning

confidence: 99%

“…The concentration of cytokinins was determined on the basis of previous experiments [3]. Explants incubated on a medium without growth regulators served as a control.…”

Section: Growth Regulator Treatmentsmentioning

confidence: 99%

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Effects of cytokinins on antioxidant enzymes in in vitro grown shoots of Pelargonium hortorum L. H. Bayley

Wojtania¹,

Skrzypek²

2014

Acta Agrobot

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“…The shoots were subcultured at three week intervals into a fresh medium in an Erlenmeyer flask and kept at a temperature of 23°C, under a 16 h photoperiod provided by cool-white fluorescent lamps at 40 μmol m ) without growth regulators (control) or supplemented with meta-topolin (mT, 2.07 μM) alone or together with a precursor of ethylene biosynthesis -1 aminocyclopropane-1-carboxylic acid (ACC, 9.9 μM), an inhibitor of ethylene synthesis -aminoethoxyvinylglycine (AVG, 5.1 μM), or an inhibitor of ethylene action -AgNO 3 (5.9 μM). The concentration of growth regulators was determined on the basis of previous experiments (Wojtania, 2010;Wojtania and Węgrzynowicz-Lesiak, 2012).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Wojtania¹,

Wegrzynowicz-Lesiak²,

Dziurka³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of Pelargonium × hortorum 'Bergpalais' in vitro. Increasing the concentration of sucrose from 15 to 40 g L -1 in medium containing meta-topolin (mT) resulted in a two-fold decrease in the number of shoots and leaves as well as a reduction in ethylene production. The addition of ethylene synthesis inhibitor (AVG) to mT-medium significantly reduced the ethylene production and the shoot growth, but it had no significant influence on the shoot formation. The mT-induced shoot formation was, however, significantly reduced in the presence of ethylene action inhibitor (AgNO 3 ), in a manner dependent on sucrose levels. At the end of the subculture period, increased sucrose concentrations (15-40 g L -1) in the presence of mT and AgNO 3 resulted in a 3.7-fold increase in ethylene emission. At the same time, the supply of sucrose caused a 2.8-fold increase in the level of endogenous abscisic acid (ABA). Our results may suggest that the inhibitory effect of high sucrose concentration (30 and 40 g L -1 ) may depend on its influence on ethylene sensitivity. It also suggests that sucrose-regulation of the shoot formation of Pelargonium in vitro is mediated by ABA.K Ke ey y w wo or rd ds s: : Ethylene inhibitors, endogenous ABA, geranium, meta-topolin, sucrose concentration, shoot multiplication.

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“…Articles published so far have enumerated the following cultivars of Pelargonium × hortorum : "Bargpalais", "Holywood Red", "Grand Prix", "Jazz Rocky Mountain", "Orbit White", "Scarlet Orbit", "Red Elite", "Ringo Rose", "Ringo Salmon", "White Rocky Mountain", and Pelargonium × hederaefolium ! "Beach", "Bonete", "Luna", "Sofie Cascade", and such like (Desiltes et al, 1993;Murthy et al, 1996;Mithila et al, 2001;Wojtania and Gabryszewska, 2001;Wojtania, 2010;Wojtania et al, 2004;Madden et al, 2005), with petals having many colors, different foliage types and growth habits. In our experiments, we focused on the elaboration of a convenient propagation medium for Pelargonium × domesticum.…”

Section: Resultsmentioning

confidence: 99%

Establishment of an in vitro culture of Pelargonium × domesticum cultivars characterized by different growth requirements

Hanus-Fajerska¹,

Mrzygłód²,

Wiszniewska³

et al. 2015

bta

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Experiments were carried out to develop micropropagation protocols for cultivars of hybrid origin which have been not cultivated via tissue culture. Proliferating cultures of Pelargonium × domesticum "Tip Top Duet" and "Black Knight" were obtained even if initially the number of excised aseptic nodal pieces was considerably reduced due to contamination with endogenic bacteria. The supplementation of a maintenance medium with either 100 or 200 mg/l peptone proved beneficial. The best propagation coefficient, exceeding 82 adventitious shoots from one initial microcutting, was obtained on a modified MS medium with an addition of 200 mg/l peptone and 0.5 mg/l adenine. The regenerated shoots readily developed new, anatomically properly formed leaves. In the plant material studied, the leaf epidermis produced glandular trichomata which were similar in structure to those observed in a standard cultivar.

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Effect of Meta-topolin on in vitro propagation of Pelargonium × hortorum and Pelargonium × hederaefolium cultivars

Cited by 45 publications

References 24 publications

Effects of cytokinins on antioxidant enzymes in in vitro grown shoots of Pelargonium hortorum L. H. Bayley

Effects of cytokinins on antioxidant enzymes in in vitro grown shoots of Pelargonium hortorum L. H. Bayley

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Establishment of an in vitro culture of Pelargonium × domesticum cultivars characterized by different growth requirements

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