Effect of Metal Cofactors of Key Enzymes on Biohydrogen Production by Nitrogen Fixing Cyanobacterium Anabaena siamensis TISIR 8012

Taikhao, Samart; Phunpruch, Saranya

doi:10.1016/j.egypro.2017.10.166

Cited by 18 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that enzyme activity is enhanced with the addition of metals in the dyebath. 33,34 Because of this, indigo baths were formulated with alkaline catalase and iron (II) sulfate as the reducing agent. Cotton was dyed in this bath following the same procedure that was used in the hydrosulfite system.…”

Section: Resultsmentioning

confidence: 99%

Indigo Dyeing of Cotton using Alkaline Catalase and Additives

Chakraborty¹,

Mazumdar²

2020

AATCC Journal of Research

View full text Add to dashboard Cite

Sodium hydrosulfite is universally applied as a reducing agent for the reduction of indigo, but also produces hazardous by-products viz. sulfate, sulfite, and thiosulfate ions with harmful effects on the environment due to their toxicity, as well as corrosive effects on the waste lines. To overcome these problems, use of an ecofriendly reducing agent, viz. alkaline catalase, along with iron (II) sulfate, was used in place of sodium hydrosulfite for dyeing of cotton with indigo. Dyed samples were characterized by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy and scanning electron microscopy (SEM). The alkaline catalase with iron (II) sulfate reducing system produced the same reduction potential in dyebaths, reduction bath stability, surface color strength, and color fastness properties of dyed cotton compared to sodium hydrosulfite use.

show abstract

Section: Resultsmentioning

confidence: 99%

Indigo Dyeing of Cotton using Alkaline Catalase and Additives

Chakraborty¹,

Mazumdar²

2020

AATCC Journal of Research

View full text Add to dashboard Cite

show abstract

“…CU2551 (Pewnual et al, 2022). With nitrogen deprivation, H 2 ase activity increased in Anabaena siamensis TISTR8012, resulting in enhanced H 2 production (Taikhao and Phunpruch 2017). On the other hand, phosphorus and sulfur deprivation did not promote H 2 production by A. halophytica.…”

Section: Type Of Mediamentioning

confidence: 98%

“…Under nitrogen deprivation, A. halophytica shows high H 2 production in dark anaerobic conditions (Taikhao et al, 2013;Taikhao et al, 2015). Moreover, nitrogen deprivation also induces H 2 evolution in Oscillatoria brevis, Calothrix membranacea (Lambert and Smith 1977), and Anabaena siamensis TISIR 8012 (Khetkorn et al, 2010;Taikhao and Phunpruch 2017). Under nitrogen deprivation, the accumulation of glycogen is carried out via photoautotrophic processes.…”

Section: Introductionmentioning

confidence: 99%

Dark fermentative hydrogen production and transcriptional analysis of genes involved in the unicellular halotolerant cyanobacterium Aphanothece halophytica under nitrogen and potassium deprivation

Chinchusak

Incharoensakdi²,

Phunpruch³

2023

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

The unicellular halotolerant cyanobacterium Aphanothece halophytica is known as a potential hydrogen (H2) producer. This study aimed to investigate the enhancement of H2 production under nutrient deprivation. The results showed that nitrogen and potassium deprivation induced dark fermentative H2 production by A. halophytica, while no differences in H2 production were found under sulfur and phosphorus deprivation. In addition, deprivation of nitrogen and potassium resulted in the highest H2 production in A. halophytica due to the stimulation of hydrogenase activity. The effect of adaptation time under nitrogen and potassium deprivation on H2 production was investigated. The results showed that the highest H2 accumulation of 1,261.96 ± 96.99 µmol H2 g dry wt−1 and maximum hydrogenase activity of 179.39 ± 8.18 µmol H2 g dry wt−1 min−1 were obtained from A. halophytica cells adapted in the nitrogen- and potassium-deprived BG11 medium supplemented with Turk Island salt solution (BG110-K) for 48 h. An increase in hydrogenase activity was attributed to the decreased O2 concentration in the system, due to a reduction of photosynthetic O2 evolution rate and a promotion of dark respiration rate. Moreover, nitrogen and potassium deprivation stimulated glycogen accumulation and decreased specific activity of pyruvate kinase. Transcriptional analysis of genes involved in H2 metabolism using RNA-seq confirmed the above results. Several genes involved in glycogen biosynthesis (glgA, glgB, and glgP) were upregulated under both nitrogen and potassium deprivation, but genes regulating enzymes in the glycolytic pathway were downregulated, especially pyk encoding pyruvate kinase. Interestingly, genes involved in the oxidative pentose phosphate pathway (OPP) were upregulated. Thus, OPP became the favored pathway for glycogen catabolism and the generation of reduced nicotinamide adenine dinucleotide phosphate (NADPH), which resulted in an increase in H2 production under dark anaerobic condition in both nitrogen- and potassium-deprived cells.

show abstract

“…In contrast, indigo requires a reduction potential of around -650 mV and more for reduction. It was determined that enzyme activity is enhanced by the addition of metals to the bath [25,26]. For this reason, indigo reduction baths were formulated with alkaline protease, together with iron (II) sulphate.…”

Section: Dyeing Of Cotton With Alkaline Proteasementioning

confidence: 99%

Dyeing of Cotton with Indigo Using Alkaline Protease and Additives

Mazumdar¹,

Chakraborty²

2019

TEK

View full text Add to dashboard Cite

Indigo is invariably applied on cotton to produce an attractive blue shade, together with the desired wash-down eff ects. Because it is water insoluble, indigo is reduced and solubilised with sodium dithionite and NaOH to develop affi nity for cotton. Sodium dithionite dissociates into hazardous by-products viz. sulphate, sulphite and thiosulphate with a harmful eff ect on the environment due to their toxicity, as well as a corrosive eff ect on waste lines. To overcome these problems, the eco-friendliness of alkaline protease, together with iron (II) sulphate (FeSO 4) as a reducing agent, was studied as a replacement for sodium dithionite. Dyed samples were characterised by attenuated total refl ection, using Fourier transformation infrared spectroscopy, scanning electron microscope and X-ray diff raction (XRD). It was observed that alkaline protease, together with iron (II) sulphate, is also capable of producing a comparable reduction potential in dye baths, reduction bath stability, and the surface colour strength and colour fastness properties of dyed cotton compared to those obtained using sodium dithionite.

show abstract

Effect of Metal Cofactors of Key Enzymes on Biohydrogen Production by Nitrogen Fixing Cyanobacterium Anabaena siamensis TISIR 8012

Cited by 18 publications

References 22 publications

Indigo Dyeing of Cotton using Alkaline Catalase and Additives

Indigo Dyeing of Cotton using Alkaline Catalase and Additives

Dark fermentative hydrogen production and transcriptional analysis of genes involved in the unicellular halotolerant cyanobacterium Aphanothece halophytica under nitrogen and potassium deprivation

Dyeing of Cotton with Indigo Using Alkaline Protease and Additives

Contact Info

Product

Resources

About