Effect of Metal Contamination on the Genetic Diversity of Deschampsia cespitosa Populations from Northern Ontario: An Application of ISSR and Microsatellite Markers

Gervais, Sophie; Nkongolo, K. K.

doi:10.5772/31164

Cited by 3 publications

(14 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They found a low level of polymorphism in D. cespitosa samples collected in 1999 from cobalt compared to samples from Sudbury. The highest level of polymorphic loci was found in samples from metal uncontaminated sites (Gervais and Nkongolo, 2011).…”

Section: Introductionmentioning

confidence: 98%

“…The five primers that were used in the analysis of the 1999 samples were selected for this ISSR analysis (Gervais and Nkongolo, 2011). They included ISSR 17898B, UBC818, UBC 827, UBC 835 and UBC 841 primers.…”

Section: Issr Analysismentioning

confidence: 99%

“…Amplified DNA products were separated on a 2% agarose gel in 0.5 xTBE containing ethidium bromide as described in Mehes et al (2007) and Gervais and Nkongolo (2011). The gel was documented with a BioRad ChemiDoc XRS system and the recorded data were analyzed using Image Lab (ver, 4.1) SoftwareTM.…”

Section: Issr Analysismentioning

confidence: 99%

“…Over time, a population can inadvertently eliminate other alleles from its gene pool. This loss of diversity can reduce its adaptation potential in the future (Mejnartowicz, 1983;Mengoni et al, 2001;Deng et al, 2007;Gervais and Nkongolo, 2011). It is therefore important to monitor the dynamics of genetic variability within and among plant populations.…”

Section: Introductionmentioning

confidence: 99%

“…Nkongolo et al (2001) and Gervais and Nkongolo (2011) assessed genetic variation in D. cespitosa populations from Northern Ontario. They found a low level of polymorphism in D. cespitosa samples collected in 1999 from cobalt compared to samples from Sudbury.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Reassessment of Molecular Variation in Isolated Populations of Deschampsia cespitosa from Metal Contaminated Regions in Northern Ontario (Canada) after 17 Years of Potential Genetic Recombination

Rainville¹,

Beckett²,

Nkongolo³

2017

American Journal of Environmental Sciences

Self Cite

View full text Add to dashboard Cite

Abstract:The effects of ore extraction and processing procedures in the Greater Sudbury and Cobalt regions have been long-lasting. The objective of this study is to determine the current level of genetic variation in Deschampsia cespitosa populations from metal contaminated and uncontaminated sites in samples collected in Northern Ontario in 2016 after 17 years of potential genetic recombination since the last study in 1999. D. cespitosa leaf samples collected from the City of Greater Sudbury (CGS), Cobalt and Little Current were analyzed using ISSR primers. The levels of genetic variation were moderate to high within targeted populations. There was no significant difference (p≤0.05) in the overall percent of polymorphic loci in metal-uncontaminated site of Little Current (from 70% in 1999 to 77% in 2016) and in a Cobalt Cart Lake site (from 48% in 1999 to 55% in 2016). But a significant decrease in genetic variation was observed in CGS Wahnapitae site (from 72% in 1999 to 54% in 2016). On the other hand, a significant increase was observed in Cobalt Nipissing (from 46% in 1999 to 64% in 2016). The Kelly Lake site in the CGS with the lowest level of polymorphic loci (42.5%) in 2016 was not surveyed in 1999. The degree of genetic relatedness among sites has increased since the populations are more genetically closely related than 17 years ago. No population-specific ISSR marker was identified. The clustering of Cobalt and Sudbury populations strengthens the earlier theory that Sudbury populations of D. cespitosa might be from the Cobalt region.

show abstract

Section: Introductionmentioning

confidence: 98%

Section: Issr Analysismentioning

confidence: 99%

Section: Issr Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Reassessment of Molecular Variation in Isolated Populations of Deschampsia cespitosa from Metal Contaminated Regions in Northern Ontario (Canada) after 17 Years of Potential Genetic Recombination

Rainville¹,

Beckett²,

Nkongolo³

2017

American Journal of Environmental Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

Genetic variation of the mangrove species Avicennia marina in heavy metal polluted estuaries of Cilegon Industrial Area, Indonesia

Manurung¹,

Siregar²,

Kusmana³

et al. 2017

Biodiversitas

View full text Add to dashboard Cite

Manurung J, Siregar IZ, Kusmana C, Dwiyanti FG. 2017. Genetic variation of the mangrove species Avicennia marina in heavy metal polluted estuaries of Cilegon Industrial Area, Indonesia. Biodiversitas 18: 1109-1115. Mangrove forests are often facing various anthropogenic disturbances, particularly in the industrial area. However, information on the status of industrial heavy metal pollution and its impact on the genetic variation of mangrove species, such as Avicennia marina, particularly in Indonesian industrial area has not been assessed. In this study, the classification of heavy metals in two estuaries of Cilegon Industrial Area (Pelabuhan Warna Sari and Krakatau) and one estuary outside Cilegon Industrial Area (Pulau Sangiang) were determined by Sediment Quality Guidelines-States Environment Protection Agency (SQGs-USEPA). Furthermore, the genetic diversity of 72 individuals of A. marina in the three estuaries were examined using four microsatellite loci (M3, M64, M81 and M98). The results showed that Krakatau area was the heavily polluted estuary based on SQGs-USEPA and have a lower genetic variation (He= 0.54) than Pelabuhan Warna Sari (moderately polluted, He= 0.56) and Pulau Sangiang (non-polluted, He= 0.60) suggesting a notable of deficiencies of genetic variation of A. marina in heavy polluted estuary compared to moderately and non-polluted estuary. Considering the low level of genetic variation in heavily polluted area, transplantation among estuaries and introduction of propagules from non-polluted estuary or other regions are needed as one of the conservation efforts.

show abstract

COMPARATIVE ANALYSIS OF INTER SIMPLE SEQUENCE REPEATS AND SIMPLE SEQUENCE REPEATS MARKERS: GENETIC ANALYSIS OF DESCHAMPSIA CESPITOSA POPULATIONS GROWING IN METAL CONTAMINATED REGIONS IN CANADA

Nkongolo¹

2014

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Comparative studies conducted on the genetic variation of metal-tolerant populations and their non-metaltolerant counterparts have been performed on numerous species using isozyme markers. Analysis of genetic differences among plant populations growing in heavy metal-contaminated and uncontaminated regions are limited. The main objectives of the present study were to compare ISSR and microsatellite markers in assessing genetic variation in D. cespitosa populations that colonized metal-contaminated and uncontaminated regions in Northern Ontario, Canada. Total genomic DNA from D. cespitosa samples were amplified with ISSR and SSR primers using optimized PCR conditions. The level of polymorphic loci varies from 46 to 74% for ISSR analysis. The level of observed heterozygosity was moderate to high ranging from 0.44 to 0.68 for the SSR primers used. But no significant difference in genetic variation levels was detected between metal contaminated and uncontaminated sites with SSR markers. There was a significant reduction of polymorphic loci in samples from highly metal-contaminated areas of the Cobalt region compared to the reference sites based on ISSR analysis. Use of a combination of different marker systems is recommended to analyse genetic variation in plant populations.

show abstract

Effect of Metal Contamination on the Genetic Diversity of Deschampsia cespitosa Populations from Northern Ontario: An Application of ISSR and Microsatellite Markers

Cited by 3 publications

References 57 publications

Reassessment of Molecular Variation in Isolated Populations of <i>Deschampsia cespitosa</i> from Metal Contaminated Regions in Northern Ontario (Canada) after 17 Years of Potential Genetic Recombination

Reassessment of Molecular Variation in Isolated Populations of <i>Deschampsia cespitosa</i> from Metal Contaminated Regions in Northern Ontario (Canada) after 17 Years of Potential Genetic Recombination

Genetic variation of the mangrove species Avicennia marina in heavy metal polluted estuaries of Cilegon Industrial Area, Indonesia

COMPARATIVE ANALYSIS OF INTER SIMPLE SEQUENCE REPEATS AND SIMPLE SEQUENCE REPEATS MARKERS: GENETIC ANALYSIS OF <i>DESCHAMPSIA CESPITOSA</i> POPULATIONS GROWING IN METAL CONTAMINATED REGIONS IN CANADA

Contact Info

Product

Resources

About