Effect of metal ions on the activity of green crab (Scylla serrata) alkaline phosphatase

Chen, Qing‐Xi; Zheng, Wen-Zhu; Lin, Jingdong; Shi, Yan; Xie, Wen-Zhang; Zhou, Hai‐Meng

doi:10.1016/s1357-2725(00)00026-1

Cited by 55 publications

(24 citation statements)

References 14 publications

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“…According with PATNI and AARONSON (1974), the estimated concentration of Hg that inhibits 50% of algal acid phosphatase activity is close to that determined in the present work. This inhibition is of similar order of magnitude to that reported for the enzyme extracted from crabs (CHEN et al, 2000).…”

Section: Figure 2 -Lineweaver-burk Plot For Hg Non-competitive Inhibisupporting

confidence: 84%

Algae Acid Phosphatase Affected by Ten Metals and Enzymatic Parameters Determination in Presence of Mercury and Copper

Jonsson

Paraíba²,

Aoyama³

2009

PES

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Acid phosphatases play important roles in algae metabolism such availability/recycling of inorganic phosphate and autophagic digestive processes. Chemicals released into the environment from agricultural activities and through industrial and urban wastes, may impair algae enzyme activity. The aim of this work was to evaluate the in vitro activation/ inhibition effect of ten metals, commonly present as contaminants in soil and water, on the acid phosphatase extracted from the green algae Pseudokirchneriella subcapitata. Results demonstrated that Hg, Al, Mo, Pb, Se and Cd inhibited the enzyme activity in 56.3, 54.5, 30.6, 25.5, 23.1 and 11.5%, respectively. This corresponds to the maximum percentage of effect attained at the metal concentrations tested (0.02-2.0 mM). On the other hand, Cu, Zn, Ni and Cr exhibited an increment on phosphatase activity equal to 95.5, 87.6, 77.6 and 42.8%, respectively. Kinetic parameters values were calculated for the metals that showed highest effects. Thus, Ki (inhibition constant) and Kd (dissociation constant) values equal to 0.0400 and 0.0016 mM were determined for Hg and Cu, respectively. A noncompetitive inhibition mechanism was attributed to the former. Results improved the understanding of the basic events of the impact of metals at biochemical levels in primary producers organisms.

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Section: Figure 2 -Lineweaver-burk Plot For Hg Non-competitive Inhibisupporting

confidence: 84%

Algae Acid Phosphatase Affected by Ten Metals and Enzymatic Parameters Determination in Presence of Mercury and Copper

Jonsson

Paraíba²,

Aoyama³

2009

PES

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“…However, when compared with our results, this parameter was about 14 times lower for the metal inhibition of the enzyme extracted from gill mollusks (Mazorra et al 2002). IC 50 values of 0.5 and 0.025 mM were determined for fish liver (El Demerdash and Elagamy 1999) and crab (Chen et al 2000) respectively.…”

Section: Resultssupporting

confidence: 54%

Metals and linear alkylbenzene sulphonate as inhibitors of the algae Pseudokirchneriella subcapitata acid phosphatase activity

Jonsson¹,

Paraíba²,

Aoyama³

2009

Ecotoxicology

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Sewage sludge applied to soils as a fertilizer often contains metals and linear alkylbenzene sulphonate (LAS) as contaminants. These pollutants can be transported to the aquatic environment where they can alter the phosphatase activity in living organisms. The acid phosphatase of algae plays important roles in metabolism such as decomposing organic phosphate into free phosphate and autophagic digestive processes. The order of in vitro inhibition of Pseudokirchneriella subcapitata acid phosphatase at the highest concentration tested was LAS > Hg2+ = Al3+ > Se4+ = Pb2+ > Cd2+. A non-competitive inhibition mechanism was obtained for Hg2+ (Ki = 0.040 mM) and a competitive inhibition for LAS (Ki = 0.007 mM). In vivo studies with treated algae cultures showed that the inhibition of specific activity was observed in algae exposed during 7 days, in contrast to short term (24 h) treatments with both these chemicals. Our results suggest that the inhibition parameters in vitro did not markedly differ between the two chemicals. On the other hand, in vivo evaluations showed strong differences between both pollutants regarding the concentration values and the degree of response.

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“…However, divalent ions as Mg, Ca, Ba, Mn, Co, Ni, Cd are able to activate this enzyme (Chen et al, 2000). AP in the cellular external membrane plays a main role in the phosphates metabolism, without this enzyme the external membrane is damaged by heavy metals (Durrieu & Tran-Minh).…”

Section: Discussionmentioning

confidence: 99%

Biochemical and Mitochondrial Changes Induced by Cd, Fe and Zn in Limnodrillus hoffmeisteri

Ortega¹,

Ordoñez²,

Favari

et al. 2011

Int. J. Morphol.

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SUMMARY:The effects of sublethal concentrations of cadmium (0.64 µg/L), iron (0.043 mg/L) and zinc (0.31 mg/L) and a mixture of these metals on succinate dehydrogenase (SD) and alkaline phosphatase (AP) activity and on structural changes in the mitochondria of epithelium cells of the digestive tract were examined in the oligochaete Limnodrillus hoffmeisteri after 96 h of exposure in artificial sediments. SD activity was significantly inhibited, particularly in treatments with Cd alone (92.57%), while AP increased its activity with Cd alone (73.23%). However, when this metal was mixed with Fe and Zn, the inhibition of SD activity was lower (67.82%) than with Cd alone, showing an antagonistic effect and AP increased its activity (73.26%). Mitochondria were structurally damaged by exposure to Cd alone. However, in the metal mixtures, the toxic effects may exert interactive effects eliciting a less structural damage in the mitochondria of epithelium cells of the digestive tract than when Cd is alone.

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Effect of metal ions on the activity of green crab (Scylla serrata) alkaline phosphatase

Cited by 55 publications

References 14 publications

Algae Acid Phosphatase Affected by Ten Metals and Enzymatic Parameters Determination in Presence of Mercury and Copper

Algae Acid Phosphatase Affected by Ten Metals and Enzymatic Parameters Determination in Presence of Mercury and Copper

Metals and linear alkylbenzene sulphonate as inhibitors of the algae Pseudokirchneriella subcapitata acid phosphatase activity

Biochemical and Mitochondrial Changes Induced by Cd, Fe and Zn in Limnodrillus hoffmeisteri

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