Effect of metal support and different carbon sources on CLA production using Lactobacillus plantarum

Razmjooei, Maryam; Shad, Ehsan; Nejadmansouri, Maryam; Safdarianghomsheh, Reza; Delvigne, Frank; Khalesi, Mohammadreza

doi:10.1016/j.bej.2020.107715

Cited by 10 publications

(3 citation statements)

References 47 publications

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“…Nitrogen expedites growth and the metabolic rate of lactobacilli, resulting in the production of lactic acid, amino acids, and protein fragments (peptides), which are considered key growth factors for lactobacilli strains [ 41 ]. Moreover, stallic acid, a type of oligosaccharide that attaches itself to cellular proteins; lactoferrin, an iron-binding glycoprotein; and calcium in calcium phosphate are enhancing tools that lactobacilli cells can use to maximize their growth rate [ 42 ]. These unique components of WBM promote the growth and health of the five tested lactobacilli strains [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Mapping a sustainable approach: biosynthesis of lactobacilli-silver nanocomposites using whey-based medium for antimicrobial and bioactivity applications

El.Fadly,

Salah,

Abdella

et al. 2024

Microb Cell Fact

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This study explores a sustainable approach for synthesizing silver nanocomposites (AgNCs) with enhanced antimicrobial and bioactivity using safe Lactobacillus strains and a whey-based medium (WBM). WBM effectively supported the growth of Lactobacillus delbrueckii and Lactobacillus acidophilus, triggering a stress response that led to AgNCs formation. The synthesized AgNCs were characterized using advanced spectroscopic and imaging techniques such as UV‒visible, Fourier transform infrared (FT-IR) spectroscopy, transmission electron (TEM), and scanning electron microscopy with energy dispersive X-ray analysis (SEM–Edx). Lb acidophilus-synthesized AgNCs in WBM (had DLS size average 817.2–974.3 ± PDI = 0.441 nm with an average of metal core size 13.32 ± 3.55 nm) exhibited significant antimicrobial activity against a broad spectrum of pathogens, including bacteria such as Escherichia coli (16.47 ± 2.19 nm), Bacillus cereus (15.31 ± 0.43 nm), Clostridium perfringens (25.95 ± 0.03 mm), Enterococcus faecalis (32.34 ± 0.07 mm), Listeria monocytogenes (23.33 ± 0.05 mm), methicillin-resistant Staphylococcus aureus (MRSA) (13.20 ± 1.76 mm), and filamentous fungi such as Aspergillus brasiliensis (33.46 ± 0.01 mm). In addition, Lb acidophilus-synthesized AgNCs in WBM exhibit remarkable free radical scavenging abilities, suggesting their potential as bioavailable antioxidants. These findings highlight the dual functionality of these biogenic AgNCs, making them promising candidates for applications in both medicine and nutrition. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Mapping a sustainable approach: biosynthesis of lactobacilli-silver nanocomposites using whey-based medium for antimicrobial and bioactivity applications

El.Fadly,

Salah,

Abdella

et al. 2024

Microb Cell Fact

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“…The source of sugar is also essential for LAB species to produce energy and cell biomass 65 . In this regard, glucose is considered the most attractive carbohydrate commonly used to enhance bacterial cell growth and lactic acid production 66 , 67 . Moreover, glucose catabolism produces severe acidification of the medium that could trigger the activation of the GAD pathway and thus, the stimulation of GABA synthesis 68 .…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of gamma-aminobutyric acid by Lactiplantibacillus plantarum K16 as an alternative to revalue agri-food by-products

Diez-Gutiérrez

Vicente

Sáenz

et al. 2022

Sci Rep

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Probiotic metabolites, known as postbiotics, have received attention due to their wide variety of promoting health effects. One of the most exciting postbiotic is gamma-aminobutyric acid (GABA), widely produced by lactic acid bacteria, due to its benefits in health. In addition, the performance of the biosynthesis of GABA by Lactiplantibacillus plantarum could be modulated through the modification of fermentation parameters. Due to their high nutritional value, agri-food by-products could be considered a useful fermentation source for microorganisms. Therefore, these by-products were proposed as fermentation substrates to produce GABA in this study. Previously, several experiments in Man Rogosa Sharpe (MRS) broth were performed to identify the most critical parameters to produce GABA using the strain Lactiplantibacillus plantarum K16. The percentage of inoculum, the initial pH, and the concentration of nutrients, such as monosodium glutamate or glucose, significantly affected the biosynthetic pathway of GABA. The highest GABA yield was obtained with 500 mM of monosodium glutamate and 25 g/L of glucose, and an initial pH of 5.5 and 1.2% inoculum. Furthermore, these investigated parameters were used to evaluate the possibility of using tomato, green pepper, apple, or orange by-products to get GABA-enriched fermented media, which is an excellent way to revalorise them.

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“…The highest concentration of conjugated linoleic acid can be obtained. This indirectly explains the promoting effect of carbon sources on the growth of lactic acid bacteria (Maryam et al., 2020). The objectives of this study were to: (1) analyze the effect of lactose on the growth of L. helveticus ; (2) explore the mechanism of the effect of lactose on the growth and metabolism of L. helveticus ; (3) establish a high‐density culture system of L. helveticus .…”

Section: Introductionmentioning

confidence: 99%

Increasing lactose concentration is a strategy to improve the proliferation of Lactobacillushelveticus in milk

Zang

Wang

Dziugan

et al. 2020

Food Science & Nutrition

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The aim of the research was to explore the effect of carbon sources on the proliferation of Lactobacillus helveticus. In this study, lactose was added to skim milk medium and the effects of carbon sources on the growth and proliferation of Lactobacillus helveticus in low‐ and high‐sugar media were compared from the aspects of metabolism‐related enzyme activity, proteomics, and transcriptomics. The results showed that under high‐sugar conditions, the rate of substance transport to cells and the Embden–Meyerhof–Parnas (EMP) pathway were significantly accelerated. The synthesis and metabolism of cells were significantly enhanced, which was beneficial to the rapid proliferation of cells. By increasing the lactose concentration in the medium and optimizing the culture method, the cell density of Lactobacillus helveticus reached 3.98 × 109 CFU/ml; a good proliferation effect was obtained.

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Effect of metal support and different carbon sources on CLA production using Lactobacillus plantarum

Cited by 10 publications

References 47 publications

Mapping a sustainable approach: biosynthesis of lactobacilli-silver nanocomposites using whey-based medium for antimicrobial and bioactivity applications

Mapping a sustainable approach: biosynthesis of lactobacilli-silver nanocomposites using whey-based medium for antimicrobial and bioactivity applications

Biosynthesis of gamma-aminobutyric acid by Lactiplantibacillus plantarum K16 as an alternative to revalue agri-food by-products

Increasing lactose concentration is a strategy to improve the proliferation of Lactobacillushelveticus in milk

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