2012
DOI: 10.3389/fmicb.2012.00155
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Effect of Metals on the Lytic Cycle of the Coccolithovirus, EhV86

Abstract: In this study we show that metals, and in particular copper (Cu), can disrupt the lytic cycle in the Emiliania huxleyi – EhV86 host–virus system. E. huxleyi lysis rates were reduced at high total Cu concentrations (> approximately 500 nM) in the presence and absence of EDTA (ethylenediaminetetraacetic acid) in acute short term exposure experiments. Zinc (Zn), cadmium (Cd), and cobalt (Co) were not observed to affect the lysis rate of EhV86 in these experiments. The cellular glutathione (GSH) content increased … Show more

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Cited by 10 publications
(14 citation statements)
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“…Using this biochemical assay, which is based on the GSH reductase-dependent reduction of 5,5′-dithiobis(2-nitro-benzoic acid) (Ellman's reagent; Tietze, 1969;Queval and Noctor, 2007) (Figures 3b-e), we detected an increase in total GSH (GSH+GSSG, Figure 3b) in cell extracts derived from lytic phase of infection. This result was supported by previous research detecting total GSH increase during infection by means of high-performance liquid chromatography (Gledhill et al, 2012). In agreement with the in vivo GSH assessment using mBCl fluorescence stain (Figure 3a), at 72 hpi levels of GSH were significantly higher in infected cells in comparison with uninfected cells (Figure 3c, Student's t-test, P-value = 0.02).…”
Section: Expression Profile Of Ros Metabolism Genes During Ehv Infectionsupporting
confidence: 79%
See 1 more Smart Citation
“…Using this biochemical assay, which is based on the GSH reductase-dependent reduction of 5,5′-dithiobis(2-nitro-benzoic acid) (Ellman's reagent; Tietze, 1969;Queval and Noctor, 2007) (Figures 3b-e), we detected an increase in total GSH (GSH+GSSG, Figure 3b) in cell extracts derived from lytic phase of infection. This result was supported by previous research detecting total GSH increase during infection by means of high-performance liquid chromatography (Gledhill et al, 2012). In agreement with the in vivo GSH assessment using mBCl fluorescence stain (Figure 3a), at 72 hpi levels of GSH were significantly higher in infected cells in comparison with uninfected cells (Figure 3c, Student's t-test, P-value = 0.02).…”
Section: Expression Profile Of Ros Metabolism Genes During Ehv Infectionsupporting
confidence: 79%
“…It was recently shown that a decrease in cellular GSH concentrations can serve as an early event in the apoptotic cascade, and that low antioxidant capacity can lead to initiation of a programmed death cascade (Circu and Aw, 2008;Franco and Cidlowski, 2009). Thus, viral replication might benefit from high intracellular GSH content (Gledhill et al, 2012), prolonging cell viability and circumventing premature cell death that might be induced as a defense strategy of the host.…”
Section: Modulation Of Host Ros Metabolismmentioning
confidence: 99%
“…Model outputs will only be of any use when rigorously tested by comparing model results against adequate data sets. Since this task relies on data availability, experimental constraints may compromise the whole modeling endeavor, such as the extreme difficulty in the quantification of virus particles in the field, for instance (Wommack et al, 1996;Gledhill et al, 2012;Heider and Metzner, 2014). Also, the difficulties related to assessing the viability of viral particles needs to be considered (Noble and Fuhrman, 2000;Bongiorni et al, 2005), given the significant number of processes involved, such as turbidity that not only affects light penetration but also passively adsorbs viruses, as recently reviewed by Mojica and Brussaard (2014).…”
Section: Final Remarksmentioning
confidence: 99%
“…A research topic exploring current research themes in environmental aquatic bioinorganic chemistry should thus incorporate articles on a diverse range of subjects. We have been honored to include both reviews and original research articles that taken together, reflect the interdisciplinary nature of the subject area and the diversity of geomes and biomes in which inorganic elements, particularly metals, play a fundamental role.We have thus been able to include articles on metals, their speciation, and interactions with phytoplankton (Cuss and Gueguen, 2012;Hassler et al, 2012; Shaked and Lis, 2012; Sunda, 2012), on metal acquisition and use by microbes (Barnett et al, 2012;Desai et al, 2012;Glass and Orphan, 2012;Morrissey and Bowler, 2012;Nuester et al, 2012;Scheidegger et al, 2012) and on the effect of inorganic ions in the environment on organism interactions and community structure (Boyd et al, 2012;Gledhill et al, 2012). Barnett, J. P., Millard, A., Ksibe, A., Scanlan, D. J., Schmid, R., and Blindauer, C. A.…”
mentioning
confidence: 99%
“…We have thus been able to include articles on metals, their speciation, and interactions with phytoplankton (Cuss and Gueguen, 2012;Hassler et al, 2012;Shaked and Lis, 2012;Sunda, 2012), on metal acquisition and use by microbes (Barnett et al, 2012;Desai et al, 2012;Glass and Orphan, 2012;Morrissey and Bowler, 2012;Nuester et al, 2012;Scheidegger et al, 2012) and on the effect of inorganic ions in the environment on organism interactions and community structure (Boyd et al, 2012;Gledhill et al, 2012).…”
mentioning
confidence: 99%