Effect of Methanol Extract of Mangifera indica on Mitochondrial Membrane Permeability Transition Pore in Normal Rat Liver and Monosodium Glutamate-induced Liver and Uterine Damage

Olowofolahan, Adeola Oluwakemi; Adeosun, Opeyemi A.; Afolabi, Olabisi; Olorunsogo, O. O.

doi:10.9734/jocamr/2018/40587

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…[14] Our previous study has shown that methanol extract of M. indica (MEMI) induces mitochondrial-mediated apoptosis (MMA) through induction of mPT pore opening and also ameliorates monosodium glutamate (MSG)-induced rat liver and uterine damage. [17] Bioactivity-guided assay in our study also revealed that the methanol fraction of M. indica (MFMI) is the most potent with respect to MMA through induction of mPT pore opening. [18] This study, therefore, investigated the effect of MFMI on oestradiol valerate (OV)-induced endometrial hyperplasia (EH) using female Wistar rats, looking at the oestrogen receptor signalling pathway.…”

Section: Introductionsupporting

confidence: 56%

Amelioration of oestradiol valerate-induced endometrial hyperplasia in female rats by methanol fraction ofMangifera indicaLinn. through modulation of oestrogen receptor signalling pathway

Olowofolahan

Tobih

Olorunsogo

2021

IJPP

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Objectives: Mangifera indica is a medicinal plant that is folklorically used in the treatment of certain disorders connected with women reproductive organs, especially, uterine fibroids. This study investigated the effect of methanol fraction of M. indica (MFMI) extract on oestradiol valerate (OV)-induced endometrial hyperplasia (EH). Materials and Methods: The animals were randomly divided into four groups of seven rats each. These include a control group, an MFMI-alone group, a model (OV-alone) group and MFMI treatment (OV+MFMI) group. The EH was induced by intraperitoneal injection of OV. The levels of oestrogen (E2), progesterone (PG) and total cholesterol (TC) were determined using ELISA technique. The uterine histological and immunohistochemical assessments of oestrogen receptor, β-catenin and Ki-67 were carried out. Fibroblast cell count/μm2 using histomorphometry as well as gas chromatography–mass spectrometry (GCMS) analysis of MFMI was carried out. Results: Severe EH was induced on oestradiol valerate administration. The MFMI was able to improve the pathological features of the animal model. Furthermore, the levels of oestrogen, PG and TC were reduced by MFMI. The immune reactive expression of oestrogen receptor alpha, β-catenin and Ki-67 was downregulated by MFMI coadministration. The histomorphometric analysis of the fibroblast cell count/μm2 showed increased cell count density in the OV-treated group which was significantly ameliorated by MFMI coadministration. The GC– MS analysis revealed the presence of some pharmacologically relevant phytochemicals. Conclusion: This study suggests that MFMI contains phytochemicals that can ameliorate OV-induced EH in female Wistar and the possible mechanism of action involves modulation of oestrogen signalling pathway.

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Section: Introductionsupporting

confidence: 56%

Amelioration of oestradiol valerate-induced endometrial hyperplasia in female rats by methanol fraction ofMangifera indicaLinn. through modulation of oestrogen receptor signalling pathway

Olowofolahan

Tobih

Olorunsogo

2021

IJPP

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Monosodium Glutamate Induces Cytotoxicity in Rat Liver via Mitochondrial Permeability Transition Pore Opening

Olowofolahan

Adeosun

Olorunsogo

2020

Cell Biochem Biophys

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Alteraciones histológicas hepáticas provocadas por la ingesta crónica de glutamato monosódico

et al. 2022

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Introducción. El glutamato es un aminoácido que está implicado en numerosas reacciones relacionadas con el metabolismo hepático, por lo que la sobreactivación de los receptores de glutamato por acción de la ingesta de glutamato monosódico (GMS) proveniente de la dieta, podría llevar a daño del tejido hepático. Este estudio se realizó con el objetivo de evaluar los cambios histológicos producidos en el hígado de ratas sometidas a la administración crónica de GMS. Metodología. Se trabajó con dos lotes de animales, uno experimental y otro control, cada uno de ellos constituido por seis ratas machos cepa Wistar de cinco semanas de edad. Al grupo experimental se le administró diariamente 0,1 g de queso de bajas calorías que contenía GMS monohidrato de 99% de pureza (grado alimentario puro), diluido en 50 μL de agua desionizada (0,3 g/100 g de peso corporal). Al grupo control se le administró la misma cantidad de sodio que el que contenía el GMS del grupo tratado, pero bajo la forma de NaCl. Al concluir el tratamiento, las ratas pertenecientes a ambos grupos se pesaron y sacrificaron, y se les extrajo el hígado para el estudio histológico. Se obtuvieron cortes histológicos que fueron coloreados con hematoxilina-eosina, PAS y coloración con tricrómico de Masson. El análisis de los cortes histológicos se llevó a cabo por observación directa en microscopio óptico con objetivo de 40x. Resultados. Se observó en general, conservación y apariencia normal de las características histológicas de los acinos hepáticos en el grupo control, en tanto que el hígado de las ratas tratadas con GMS presentó diferentes grados de degeneración hidrópica, cantidades variables de cuerpos hialinos eosinófilos, infiltración inflamatoria de células mononucleares y necrosis focal, principalmente en la zona 1 del acino hepático. Conclusión. Los resultados encontrados permiten aportar evidencias en torno a las alteraciones histopatológicas que la ingesta crónica de GMS provoca sobre el tejido hepático. Se recomienda alertar a la población para reducir la ingesta de alimentos que poseen GMS como saborizante.

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Effect of Methanol Extract of Mangifera indica on Mitochondrial Membrane Permeability Transition Pore in Normal Rat Liver and Monosodium Glutamate-induced Liver and Uterine Damage

Cited by 3 publications

References 14 publications

Amelioration of oestradiol valerate-induced endometrial hyperplasia in female rats by methanol fraction ofMangifera indicaLinn. through modulation of oestrogen receptor signalling pathway

Amelioration of oestradiol valerate-induced endometrial hyperplasia in female rats by methanol fraction ofMangifera indicaLinn. through modulation of oestrogen receptor signalling pathway

Monosodium Glutamate Induces Cytotoxicity in Rat Liver via Mitochondrial Permeability Transition Pore Opening

Alteraciones histológicas hepáticas provocadas por la ingesta crónica de glutamato monosódico

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