Effect of methanolic extract of sphaeranthus amaranthoides (MESA) on nicotine-induced Serum testosterone levels in adult Wistar albino rats

Abstract

Background: Infertility is one of the most common health issues. Infertility in men occurs when sperms cannot fertilize eggs. Infertility can be caused by a variety of factors, medical conditions, and medications. Testicular insufficiency results when a normal individual is exposed to some toxic substances and lifestyle disorders. Smoking cigarette is one such condition that affects male reproductive system. Nicotine is the main metabolite of cigarette smoke. Testicular damages are always related with hormonal changes. Objectives: The aim of this study is to evaluate the effects of Methanolic extract of sphaeranthus amaranthoides with regard to Changes in serum testosterone levels caused as a result of nicotine -induced testicular damage. Methods: Four equal groups( 24nos) of male wistar albino rats were randomly assigned to control, nicotine and methanol extract of sphaeranthus amaranthoides with doses1(250 mg/kg BW) and 2 (500 mg/kg BW), respectively. The animals were treated for forty-eight days. The blood samples were collected at the interval of 20, 40, and 48 days, to evaluate the serum testosterone levels. Results: Nicotine reduced Serum testosterone level (p < 0.001).When treated along with Methanolic extract of sphaeranthus amaranthoides treatment significantly increased blood testosterone level (p<0.001).