Effect of Methionine Analogues on Growth Performance, Serum Biochemical Parameters, Serum Free Amino Acids and Rumen Fermentation of Yaks

Zhang, Xirui; Zuo, Zizhen; Liu, Yao; Wang, Chenxi; Peng, Zhongli; Zhong, Jincheng; Zhang, Ming; Wang, Haibo

doi:10.3390/ani12223175

Cited by 8 publications

(5 citation statements)

References 65 publications

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“…In this study, RPML supplementation increased MDA concentrations when yaks were fed the low-protein diet, while MDA concentrations were not affected when yaks were fed the high-protein diet. These results may indicate that excessive Met may cause lipid peroxidation and production of MDA [ 45 ]. In this study, RPML supplementation increased the activities of T-AOC, and tended to increase the activities of CAT.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Protein Level and Rumen-Protected Methionine and Lysine on Growth Performance, Rumen Fermentation and Serum Indexes for Yaks

Wang,

Fu,

et al. 2024

Animals

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This study investigated the effects of the dietary protein level and rumen-protected methionine and lysine (RPML) on the growth performance, rumen fermentation, and serum indexes of yaks. Thirty-six male yaks were randomly assigned to a two by three factorial experiment with two protein levels, 15.05% and 16.51%, and three RPML levels: 0% RPML; 0.05% RPMet and 0.15% RPLys; and 0.1% RPMet and 0.3% RPLys. The trial lasted for sixty days. The results showed that the low-protein diet increased the DMI and feed conversion ratio of yaks. The diet supplemented with RPML increased the activities of IGF1 and INS and nutrient digestibility. The high-protein diet decreased the rumen butyrate concentration and increased the rumen isovalerate concentration. The low-protein diet supplemented with RPML increased the rumen pH and the concentrations of total volatile fatty acids, butyrate and NH3-N; the high-protein diet supplemented with a high level of RPML decreased the rumen pH and the concentrations of isobutyrate, isovalerate, propionate and NH3-N. The low-protein diet supplemented with RPML increased the total antioxidant capacity and glutathione peroxidase activity, along with the concentrations of malondialdehyde and amino acids such as aspartic acid, lysine, cysteine, etc. In conclusion, a low-protein diet supplemented with RPML is beneficial for rumen and body health, physiological response, and metabolic status in yaks.

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Section: Discussionmentioning

confidence: 99%

Effects of Dietary Protein Level and Rumen-Protected Methionine and Lysine on Growth Performance, Rumen Fermentation and Serum Indexes for Yaks

Wang,

Fu,

et al. 2024

Animals

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“…But had minimal impact on other biochemical indicators, which were consistent with previous studies ( 4 , 57 ). BUN, an amino acid and protein metabolite, can serve as an indicator of the equilibrium of protein and amino acid metabolism within an animal ( 58 ). BUN of ruminants is mainly derived from ammonia produced by rumen degradable protein (RDP) and the metabolites of rumen undegradable protein (UDP) that entering the stomach and small intestine.…”

Section: Discussionmentioning

confidence: 99%

Effects of nanoselenium on the performance, blood indices, and milk metabolites of dairy cows during the peak lactation period

Xiao,

Wang,

Wei

et al. 2024

Front. Vet. Sci.

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To compare the impact of nanoselenium and sodium selenite on the performance, blood indices, and milk metabolites of dairy cows during the peak lactation period, two groups of dairy cows under the same conditions were selected as the control group (CON group) and treatment group (NSe group) for a 38-day (10 days for adaptation and 28 days for sampling) experiment. The control group (CON) was provided a basal diet +3.3 g/d of sodium selenite (purity1%), whereas the nanoselenium group (NSe) was offered the same diet +10 mL/d of nanoselenium (selenium concentration 1,500 mg/L). The results showed that NSe significantly increased the milk yield, milk selenium content, and feed efficiency (p < 0.05), but had no significant effect on other milk components (p > 0.05). NSe significantly increased blood urea nitrogen (BUN) and alkaline phosphatase (ALP) (p < 0.05), but had no significant effects on malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), blood total antioxidant capacity (T-AOC), or blood selenium (p > 0.05). In addition, the nontargeted metabolomics of the milk was determined by LC–MS technology, and the differentially abundant metabolites and their enrichment pathways were screened. According to these findings, NSe considerably increased the contents of cetylmannoside, undecylenoic acid, 3-hydroxypentadecanoic acid, 16-hydroxypentadecanoic acid, threonic acid, etc., but decreased the contents of galactaric acid, mesaconic acid, CDP-glucose etc. Furthermore, the enriched metabolic pathways that were screened with an impact value greater than 0.1 included metabolism of niacin and niacinamide, pyruvate, citrate cycle, riboflavin, glycerophospholipid, butanoate and tyrosine. Pearson correlation analysis also revealed a relationship between different milk metabolites and blood selenium, as well as between milk selenium and blood biochemical indices. In conclusion, compared with sodium selenite, nanoselenium improves the milk yield, feed efficiency, and milk selenium content of dairy cows and regulates milk metabolites and related metabolic pathways in Holstein dairy cows during the peak lactation period, which has certain application prospects in dairy production.

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“…Rutin, or rutoside, quercetin-3-O-rutinoside, or vitamin P, is a natural flavonoid glycoside that is a secondary metabolite in plants, which has antioxidant, anti-hypertension, anti-diabetes, anti-inflammation, and cardioprotective functions. Previously, we reported that rutin promoted the growth performance of sheep by enhancing antioxidant capacity and rumen fermentation and regulating hormone secretion, and improved meat quality of goats [4][5][6]. These studies indicate that rutin might be used in ruminant production as a feed additive.…”

Section: Introductionmentioning

confidence: 93%

“…Cell suspensions (2 mL) containing 1×10 5 GRECs/mL were inoculated into 12-well plates. The basal medium was replaced by the treatment medium after cell attachment.…”

Section: Anti-oxidative Parametersmentioning

confidence: 99%

Rutin alleviated lipopolysaccharide-induced damage in goat rumen epithelial cells

Zhan,

Gu,

Wang

et al. 2024

Anim Biosci

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Objective: Rutin, also called vitamin P, is a flavonoids from plants. Previous studies have indicated that rutin can alleviate the injury of tissues and cells by inhibiting oxidative stress and ameliorating inflammation. There is no report on the protective effects of rutin on goat rumen epithelial cells (GRECs) at present. Hence, we investigated whether rutin can alleviate lipopolysaccharide (LPS)-induced damage in GRECs.Methods: GRECs were cultured in basal medium or basal medium containing 1 μg/mL LPS, or 1 μg/mL LPS and 20 μg/mL rutin. Six replicates were performed for each group. After 3-h culture, the GRECs were harvested to detect the relevant parameters.Results: Rutin significantly enhanced the cell activity (p<0.05) and transepithelial electrical resistance (TEER) (p<0.01) and significantly reduced the apoptosis rate (p<0.05) of LPSinduced GRECs. Rutin significantly increased superoxide dismutase, glutathione peroxidase, and catalase activity (p<0.01) and significantly decreased lactate dehydrogenase activity and reactive oxygen species and malondialdehyde (MDA) levels in LPS-induced GRECs (p<0.01). The mRNA and protein levels of interleukin 6 (IL-6), IL-1β, and C-X-C motif chemokine ligand 8 (CXCL8) and the mRNA level of tumor necrosis factor-α (TNF-α) and chemokine C-C motif ligand 5 (CCL5) were significantly increased in LPS-induced GRECs (p<0.05 or p<0.01), while rutin supplementation significantly decreased the mRNA and protein levels of IL-6, TNF-α, and CXCL8 in LPS-induced GRECs (p<0.05 or p<0.01). The mRNA level of toll-like receptor 2 (TLR2), and the mRNA and protein levels of TLR4 and nuclear factor κB (NF-κB) was significantly improved in LPS-induced GRECs (p<0.05 or p<0.01), whereas rutin supplementation could significantly reduce the mRNA and protein levels of TLR4 (p<0.05 or p<0.01). In addition, rutin had a tendency of decreasing the protein levels of CXCL6, NF-κB, and inhibitor of nuclear factor kappa-B alpha (0.05< p<0.10). Rutin could significantly decreased interferon regulatory factor 3 mRNA expression in LPS-induced GRECs (p<0.05), whereas interferon induced protein with tetratricopeptide repeats 3 (IFIT3) and toll-interacting protein (TOLLIP) mRNA expression was not significantly different between the groups. LPS reduced the tight junction protein zonula occludin 1 (ZO-1) level in GRECs whereas rutin enhanced it. Rutin significantly improved tight junction protein <i>Claudin-1</i> mRNA expression in LPS-induced GRECs (p<0.01), but could not affect tight junction protein <i>Occludin</i> mRNA expression.Conclusion: Rutin alleviated LPS-induced barrier damage in GRECs by improving oxidation resistance and anti-inflammatory activity, which may be related to TLR/NF-κB signaling pathway inhibition.

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Effect of Methionine Analogues on Growth Performance, Serum Biochemical Parameters, Serum Free Amino Acids and Rumen Fermentation of Yaks

Cited by 8 publications

References 65 publications

Effects of Dietary Protein Level and Rumen-Protected Methionine and Lysine on Growth Performance, Rumen Fermentation and Serum Indexes for Yaks

Effects of Dietary Protein Level and Rumen-Protected Methionine and Lysine on Growth Performance, Rumen Fermentation and Serum Indexes for Yaks

Effects of nanoselenium on the performance, blood indices, and milk metabolites of dairy cows during the peak lactation period

Rutin alleviated lipopolysaccharide-induced damage in goat rumen epithelial cells

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