Effect of Methyl Jasmonate on antioxidative enzyme activities and on the contents of ROS and H2O2 in Ricinus communis leaves

Abstract

Jasmonates are a class of plant hormones that mediate various aspects in gene and metabolic regulation, defense, stress responses, reproduction and, possibly, communication. Oxidative stress stimulates synthesis of antioxidant metabolites and enhances antioxidant enzyme activities that could protect plant tissues. The aim of this study was to verify the effects of methyl jasmonate (JAME) treatment on the reactive oxygen species (ROS) and on the activities of H 2 O 2 scavenging enzymes, such as superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX EC; 1.11.1.1), and guaiacol peroxidase (GPX; EC 1.11.1.7) in Ricinus communis leaves. The activity of CAT and GPX was transient while SOD activity decreased and APX increased after treatment with JAME. In addition, JAME exposure induced ROS accumulation.

Exogenous application of methyl jasmonate lowers the effect of cadmium-induced oxidative injury in rice seedlings

“…Jasmonates are known to mediate and regulate major stress responses. The role of jasmonates and its derivatives in plants has received considerable attention and various modes of action have been discussed (Farmer and Ryan, 1990;Farmer, 2007;Cao et al, 2009;Soares et al, 2010;Guo et al, 2013;Zhou et al, 2013;Gill et al, 2013). Oxidative stress stimulates synthesis of antioxidant metabolites and enhances antioxidant enzyme activities that could protect plant tissues.…”

“…The variations in antioxidant activity within the cell can modify ROS content leading into death or acclimatization response. Soares et al (2010) reported a 33% increase in ROS 1 h after treatment with MeJA in Ricinus communis with decreased activities of CAT and POD in these plants. Accumulation and synthesis of stress-specific defense related protein in Cd-stressed plants are well established (Shah and Dubey, 1998).…”

“…Jasmonates (JA) are a class of plant hormones that mediate various aspects of gene and metabolic regulation, stress responses, reproduction, defense and cell communication (Soares et al, 2010). Jasmonic acid and its volatile methyl ester, methyl jasmonate (MeJA), are a class of cyclopentanone compounds, regarded as endogenous regulators that play important roles for regulating the stress response, plant growth and development (Creelman and Mullet, 1997).…”

“…In recent research, MeJA has been reported to reduce the development of chilling injury symptoms in a number of horticultural crops, including mango, sweet pepper and tomato fruit. These phytohormones induce the production of a wide array of direct and indirect chemicals such as pathogenesis-related and cellular protection molecules, including proteins involved in detoxification and redox balance, proteinase inhibitors, antimicrobial secondary metabolites, antioxidants and toxins (Farmer and Ryan 1990;Farmer, 2007;Cao et al, 2009;Soares et al, 2010;Guo et al, 2013;Zhou et al, 2013;Gill et al, 2013).…”

“…Plants continuously sense and assess the level of ROS and reprogram their gene expression to respond to the changing conditions in the environment (Xiang and Oliver, 1998;Soares et al, 2010). The variations in antioxidant activity within the cell can modify ROS content leading into death or acclimatization response.…”