Effect of Methyl Parathion on the Population Growth of the Rotifer Brachionus patulus (O. F. Müller) under Different Algal Food (Chlorella vulgaris) Densities

Sarma, S. S. S.; Gama-Flores, José Luis

doi:10.1006/eesa.2000.2014

Cited by 31 publications

(19 citation statements)

References 25 publications

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“…Thus the safe concentration derived from the frequently used application factor (0.01 · LC 50 ) is still toxic to B. patulus regardless of the turbidity levels (Mangas-Ramírez et al, 2004). Many studies have emphasized the need to derive safe concentrations based on sub-lethal testing rather than acute toxicity tests (Gama-Flores et al, 1999;Sarma et al, 2001). Our results support this approach.…”

Section: Discussionsupporting

confidence: 86%

Combined effects of sediment and lead (PbCl2) on the demography of Brachionus patulus (Rotifera: Brachionidae)

García-García¹,

Picazo-Paez

Nandini

2007

Hydrobiologia

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We studied the response of Brachionus patulus to different concentrations of the heavy metal Pb in the presence and absence of sediments. We conducted acute (LC 50 ) and chronic (life table demography and population growth) toxicity tests using sediment levels of 0, 30 and 280 mg l À1 (=0, 17 and 170 NTU) and Pb at 0, 0.06 and 0.6 mg l À1 . Experiments were conducted at 20 ± 1°C on a horizontal shaker and algal food (Chlorella vulgaris) was added at a density of 1.0 · 10 6 cells ml À1 . The median lethal concentration (LC 50 ± 95% Confidence intervals) of PbCl 2 for B. patulus was 6.15 ± 1.08 mg l À1 . Age-specific survivorship and fecundity curves showed increase in turbidity level resulted in decreased survival and offspring production of the rotifers. Increase in Pb concentration too had a negative effect on the survival and reproductive output of B. patulus. Statistically, average lifespan, life expectancy at birth, gross and net reproductive rates and the rate of population increase were all significantly influenced by the concentration of Pb, turbidity level as well as the interaction of Pb concentration · turbidity level. Rotifers exposed to 170 NTU did not grow regardless of the heavy metal concentration in the medium. Similarly, B. patulus exposed to 0.6 mg l À1 Pb did not survive beyond 10 days regardless of the turbidity level in the medium. The rate of population increase of B. patulus derived from the growth experiments was negative in all treatments containing Pb as low as 0.06 mg l À1 or turbidity level as low as 17 NTU. In treatments containing Pb or sediments, there existed no relation between the egg ratio and the population density.

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Section: Discussionsupporting

confidence: 86%

Combined effects of sediment and lead (PbCl2) on the demography of Brachionus patulus (Rotifera: Brachionidae)

García-García¹,

Picazo-Paez

Nandini

2007

Hydrobiologia

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“…Our observations of high densities of small (<300 µm) zooplankton, principally rotifers and a paucity of large (>1000 µm) zooplankton such as cladocerans in lake Xochimilco (Nandini et al, 2005) are in accordance with the published literature on fish predation effects on plankton communities (Gliwicz, 2003). While rotifer populations are more resistant to these changes (Sarma et al, 2001;RamirezPerez et al, 2004), they are not the preferred prey of axolotls (Chaparro-Herrera et al, 2011). The above is just a small part of all the problems that exist for future reintroduction in lake Xochimilco.…”

Section: Heterocypris Incongruensmentioning

confidence: 99%

Effect of water quality on the feeding ecology of axolotl Ambystoma mexicanum

Chaparro-Herrera¹,

Nandini²,

Sarma³

2013

J Limnol

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“…In the cladocerans Daphnia pulex (Dodson et al, 1995;Hanazato and Hirokawa, 2004) and D. magna (Hanazato and Yasuno, 1987;Fernández-Casalderrey et al, 1995), organophosphate and carbamate pesticides alter their population dynamics, disrupting structure and function of the aquatic community. The same effect can be observed in the rotifers Brachionus calyciflorus, B. angularis, and B. patulus exposed to methyl-parathion (Fernández-Casalderrey et al, 1993;Flores et al, 1999;Sarma et al, 2001;Flores et al, 2004) and the rotifer Lecane quadridentata exposed to carbaryl and methyl-parathion (Pérez-Legaspi et al, 2012). Thus, since rotifers are aquatic invertebrates with cholinergic (Nogrady and Alai, 1983;Raineri, 1984;Pineda-Rosas et al, 2005), catecholaminergic (Kotikova 1995), and serotoninergic (Kotikova et al, 2005) nervous system, there are susceptible to toxic effects of these pesticides in the aquatic environment.…”

Section: Introductionmentioning

confidence: 58%

Reduced expression of exocytotic proteins caused by anti-cholinesterase pesticides in Brachionus calyciflorus (Rotifera: Monogononta)

2015

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The organophosphate and carbamate pesticides methyl-parathion and carbaryl have a common action mechanism: they inhibit acetylcholinesterase enzyme by blocking the transmission of nerve impulses. However, they can alter the expression of exocytotic membrane proteins (SNARE), by modifying release of neurotransmitters and other substances. This study evaluated the adverse effects of the pesticides methyl-parathion and carbaryl on expression of SNARE proteins: Syntaxin-1, Syntaxin-4 and SNAP-23 in freshwater rotifer Brachionus calyciflorus. Protein expression of these three proteins was analyzed before and after exposure to these two pesticides by Western Blot. The expression of Syntaxin-1, Syntaxin-4 and SNAP-23 proteins in B. calyciflorus significantly decreases with increasing concentration of either pesticides. This suggests that organophosphates and carbamates have adverse effects on expression of membrane proteins of exocytosis by altering the recognition, docking and fusion of presynaptic and vesicular membranes involved in exocytosis of neurotransmitters. Our results demonstrate that the neurotoxic effect of anticholinesterase pesticides influences the interaction of syntaxins and SNAP-25 and the proper assembly of the SNARE complex.Keywords: SNARE, pesticide, anticholinesterase, neuroexocytosis, rotifer. Redução da expressão de proteínas de exocitose causados por pesticidas anti-colinesterase em Brachionus calyciflorus (Rotifera: Monogononta) ResumoOs pesticidas organofosforados e carbamatos metil-paration e carbaril tem um mecanismo de ação comum: eles inibem a enzima acetilcolinesterase, bloqueando a transmissão dos impulsos nervosos. No entanto, eles podem alterar a expressão de proteínas de membrana de exocitose (SNARE), através da modificação da libertação de neurotransmissores e outras substâncias. Este estudo avaliou os efeitos adversos dos pesticidas metil-paration e carbaril sobre a expressão de proteínas SNARE: Sintaxina -1, Sintaxina-4 e SNAP-23 em rotíferos de água doce Brachionus calyciflorus. A expressão destas três proteínas foi analisada antes e depois da exposição a estes dois pesticidas por Western Blot. A expressão das proteínas Sintaxina-1, Sintaxina-4 e SNAP-23 em B. calyciflorus diminui significativamente com o aumento da concentração de ambos os pesticidas. Isto sugere que os organofosfatos e carbamatos têm efeitos adversos sobre a expressão de proteínas de membrana de exocitose, alterando o reconhecimento, de encaixe e fusão de membranas pré-sinápticas e vesiculares envolvidas na exocitose de neurotransmissores. Nossos resultados demonstram que o efeito neurotóxico de pesticidas anticolinesterásicos influencia a interação de sintaxinas e SNAP-25 e a montagem correta do complexo SNARE.

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Effect of Methyl Parathion on the Population Growth of the Rotifer Brachionus patulus (O. F. Müller) under Different Algal Food (Chlorella vulgaris) Densities

Cited by 31 publications

References 25 publications

Combined effects of sediment and lead (PbCl2) on the demography of Brachionus patulus (Rotifera: Brachionidae)

Combined effects of sediment and lead (PbCl2) on the demography of Brachionus patulus (Rotifera: Brachionidae)

Effect of water quality on the feeding ecology of axolotl Ambystoma mexicanum

Reduced expression of exocytotic proteins caused by anti-cholinesterase pesticides in Brachionus calyciflorus (Rotifera: Monogononta)

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