Effect of Metronidazole on the Intestinal Microflora of the American Cockroach, Periplaneta americana L

Abstract

The effect of feeding to cockroaches the antimicrobial agent metronidazole, which acts specifically against anaerobes, was assessed by light and scanning electron microscopy, bacteriological examination, and methane formation. Types of organisms and total numbers were greatly reduced from controls. The health of mature adults was unaffected, but stunting occurred in subadult animals maintained on the antibiotic from hatching. The intracellular bacteria of the fat body were not affected by the drug. The results are discussed with respect to a proposed microbe-host extracellular symbiosis.Studies of the alimentary canal of the cockroach indicate the presence of an elaborate hindgut microflora (7). Many of the attached microbes and unusual morphological forms have been refractory to isolation. As an alternative to the isolation and characterization of all the anaerobes possible, we have approached the problem of interactions of the hindgut flora with the animal via the possibilities of transport of microbial products across the hindgut wall (J. W. Bracke and A.

Isolation and characterization of a Chryseobacterium strain from the gut of the American cockroach, Periplaneta americana

“…The gut of P. americana contains a dense and morphologically and functionally diverse microbial population contributing to the host development (Bracke et al 1978;Bracke and Markowetz 1980;Gijzen and Barughare 1992;Zurek andKeddie 1996, 1998;Zurek 1998).…”

The Core Gut Microbiome of the American Cockroach, Periplaneta americana, Is Stable and Resilient to Dietary Shifts

“…This hindgut microbial community breaks down recalcitrant dietary components from food that has passed through the fore-and midgut, supplying the cockroach with volatile fatty acids such as acetate (7). While this is not thought to be an obligate symbiosis, reducing the gut microbiota in Periplaneta americana slows development and results in lowered body weight and metabolic activity, suggesting that the gut microbiota plays an important role in the health and fitness of cockroaches (7)(8)(9). Recent work also suggested that the hindgut microbiota is responsible for producing pheromones, including volatile fatty acids, which promote social behavior among cockroaches (10).…”

Characteristics of Enterobacteria from Harmonia axyridis and Effects of Staphylococcus spp. on Development of H. axyridis

“…는데, 진딧물 (Buchner, 1965;Baumann and Baumann, 1994;Munson et al, 1991a), 바퀴벌레 (Bracke et al, 1979), 노린재 (Prado et al, 2006(Prado et al, , 2009 (Munson et al, 1991b;Moran et al, 1993). 따라서 진딧물의 먹이에 tetracycline을 처 리하여 Buchnera균의 생장을 억제하고 제거하였을 때 기주의 생장이 느리고 자손을 생산하지 못하는 것을 보여 주었다 (Mittler, 1971).…”

“…진행되고 있다 (Werren, 1997;Douglas, 1998;Dillon and Dillon, 2004). (Mittler, 1971;Wilkinson, 1998;Koga et al, 2003;Jim and Peter, 2007), 바퀴 (Bracke et al, 1979) Literature Cited…”